Quantitative analysis of the distribution of serotonin-immunoreactive cell bodies in the mouse brain
Abstract
The distribution of serotonin neurons in the mouse brain was studied using a peroxidase-antiperoxidase (PAP) immunohistochemical method to obtain quantitative data on serotonin-containing cell bodies. The average total number of perikarya of serotonin neurons was about 26,000, of which about 17,000 cells (66.9%) were situated in the raphe system. The nucleus raphe dorsalis was the main region where a large number of serotonin neurons were present, and it contained about 9000 cells. Other than the raphe system, serotonin-immunoreactive perikarya were seen in the following regions: the reticular formation (18.9%), the lemniscus medialis (8.5%), the nucleus interpeduncularis (1.5%), the subpyramidal region (4.0%) and the periaqueductal gray (0.2%).
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The DR contains about 35% of the approximately 26,000 serotonin-producing neurons in the mouse brain and is the predominant source of serotonergic innervation of the forebrain.
“The dorsal raphe nucleus (DR) contains ~35% of ~26,000 serotonin-producing neurons in the mouse brain and is the predominant source of serotonergic innervation of the forebrain (Ishimura et al., 1988).”
From Anatomically defined and functionally distinct dorsal raphe serotonin sub-systems (2018) · cites this paper as Ishimura et al., 1988
Collectively, ~12,000 serotonin neurons from the DR and MR in mice (~0.015% of all CNS neurons) innervate the entire forebrain (Ishimura et al., 1988) and modulate diverse physiological and behavioral functions.
“Collectively,~12,000 serotonin neurons from the DR and MR in mice (~0.015% of all CNS neurons) innervate the entire forebrain (Ishimura et al., 1988) and modulate diverse physiological and behavioral functions.”
From Single-cell transcriptomes and whole-brain projections of serotonin neurons in the mouse dorsal and median raphe nuclei (2019) · cites this paper as Ishimura et al., 1988
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- Authors
- Ishimura, K., Takeuchi, Y., Fujiwara, K., Tominaga, M., Yoshioka, H., & Sawada, T.
- Affiliations
- Kyoto Prefectural University of Medicine (JP)
- Corresponding author
- Takeuchi, Yoshihiro
- ORCID
- Yoshioka, Hiroshi
- Venue
- Neuroscience Letters
- Publisher
- Elsevier BV
- Published
- 1988-09
- Volume
- 91
- Issue
- 3
- Pages
- 265-270
- ISSN
- 0304-3940 (ISSN-L)
- OpenAlex
- W2080040046
- Cited by
- 142
- References
- 19
- Citations by year
- 2026: 5 · 2025: 9 · 2024: 7 · 2023: 18 · 2022: 9 · 2021: 14 · 2020: 13 · 2019: 6 · 2018: 9 · 2017: 4 · 2016: 3 · 2015: 5 · 2014: 1 · 2013: 2 · 2012: 2
- FWCI
- 0.96
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Neuroscience Letters (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurotransmitter Receptor Influence on Behavior
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
- Keywords
- serotonergic neurons, raphe nuclei, dorsal raphe nucleus, reticular formation, periaqueductal gray, mouse brain, neuronal distribution
- MeSH terms
- Animals, Brain, Cell Count, Immunohistochemistry, Male, Mice, Inbred ICR, Neurons, Serotonin, Mice
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