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Identification of some bacterial cell components by FT-IR spectroscopy
Open accessAbstract
Some particular cell components can be detected and identified by Fourier Transform infrared spectroscopy (FT-IR) of intact bacteria. Typical marker bands were used to identify these bacterial cell components. Polypeptide capsules were detected in several Bacillus species by a band typical for α-helical structures and by strong carboxylate stretching vibrations. Formation of endospores in clostridia and bacilli was discovered using marker bands for dipicolinic acid. Spectra of some Bacillus strains showed expression of poly-β-hydroxybutyric acid granules, capsules and endospores simultaneously.
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Smoothing and Differentiation of Data by Simplified Least Squares Procedures.
Savitzky, A., & Golay, M. J. E. (1964) · Analytical Chemistry · doi:10.1021/ac60214a047 · Cited by 21,904
Cited by
Omoike, A., & Chorover, J. (2004) · Biomacromolecules · doi:10.1021/bm034461z · Cited by 444
Wang, Y., Li, C., Liu, P., Ahmed, Z., Xiao, P., & Bai, X. (2010) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2010.06.013 · Cited by 407
Kiwi, J., & Nadtochenko, V. (2005) · Langmuir · doi:10.1021/la046983l · Cited by 318
Goodacre, R., Shann, B., Gilbert, R. J., Timmins, É. M., McGovern, A. C., Alsberg, B. K., Kell, D. B., & Logan, N. A. (2000) · Analytical Chemistry · doi:10.1021/ac990661i · Cited by 305
Liu, J., & Huang, Q. (2016) · Applied Spectroscopy · doi:10.1177/0003702816645605 · Cited by 45
Metadata
- Authors
- Helm, D.
- Venue
- FEMS Microbiology Letters
- Publisher
- Oxford University Press (OUP)
- Published
- 1995-02-01
- Volume
- 126
- Issue
- 1
- Pages
- 75-79
- ISSN
- 0378-1097 (ISSN-L)
- OpenAlex
- W1968636361
- Cited by
- 206
- References
- 1
- FWCI
- 1.49
- Type
- journal article
- Language
- en
- Open access
- bronze
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