Characterization and quantitation of the active polynucleotide fraction (PDRN) from human placenta, a tissue repair stimulating agent
Abstract
The polydeoxyribonucleotide (PDRN) fraction is an extract which forms the active component in a new formulation of the drug Placentex (a tissue repair stimulating agent), obtained from human placenta through an original proprietory extraction method. From a comparison of the UV, NMR and IR spectra of this fraction (before and after nuclease treatment) with that of a similar standard (Sigma D1501), it was shown that the active substances in the PDRN fraction mainly consist of a mixture of DNA fragments. By gel electrophoresis, the molecular weights of the DNA fragments were shown to range from 50 to 2000 base pairs. Finally, an HPLC method is described, based on an anion-exchange material capable of determining the amount of PDRN in different batches of the extract, which varied from 80 to 90%.
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No standardized criteria have been established to delineate molecular weight thresholds that clearly distinguish PN from PDRN.
“To date, no standardized criteria have been established to delineate molecular weight thresholds that clearly distinguish PN from PDRN, despite considerable variation among previously proposed classifications [11,20,77,143].”
From Comparison of Polynucleotide and Polydeoxyribonucleotide in Dermatology: Molecular Mechanisms and Clinical Perspectives (2025) · cites this paper as [143]
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- Authors
- Tonello, G., Daglio, M., Zaccarelli, N., Sottofattori, E., Mazzei, M., & Balbi, A.
- Affiliations
- Mastelli (Italy) (IT)
- University of Genoa (IT)
- Corresponding author
- Balbi, Alessandro
- ORCID
- Balbi, Alessandro
- Venue
- Journal of Pharmaceutical and Biomedical Analysis
- Publisher
- Elsevier BV
- Published
- 1996-08
- Volume
- 14
- Issue
- 11
- Pages
- 1555-1560
- ISSN
- 0731-7085 (ISSN-L)
- OpenAlex
- W1970005289
- Cited by
- 111
- References
- 0
- Citations by year
- 2026: 7 · 2025: 10 · 2024: 11 · 2023: 4 · 2022: 3 · 2021: 5 · 2020: 3 · 2019: 2 · 2018: 5 · 2017: 6 · 2016: 3 · 2015: 6 · 2014: 4 · 2013: 5 · 2012: 9
- FWCI
- 0.79
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Pharmaceutical and Biomedical Analysis (publishedVersion)
- PubMed (publishedVersion)
- CINECA IRIS Institutial Research Information System (University of Genoa) (submittedVersion)
- Topics
- Physical Sciences › Chemistry › Analytical Chemistry › Analytical Methods in Pharmaceuticals
- Health Sciences › Medicine › Rehabilitation › Wound Healing and Treatments
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Molecular Biology Techniques and Applications
- Keywords
- polydeoxyribonucleotide, human placenta extract, DNA fragments, nuclease treatment, anion-exchange HPLC, molecular weight distribution
- MeSH terms
- Chromatography, High Pressure Liquid, DNA, Humans, Molecular Weight, Placental Extracts, Polydeoxyribonucleotides, Reproducibility of Results
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