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Agrin released by motor neurons induces the aggregation of acetylcholine receptors at neuromuscular junctions
Abstract
To test the hypothesis that agrin mediates motor neuron-induced aggregation of acetylcholine receptors (AChRs) in skeletal muscle fibers and to determine whether the agrin active in this process is released by motor neurons, we raised polyclonal antibodies to purified ray agrin that blocked its receptor aggregating activity. When the antibodies were applied to chick motor neuron--chick myotube cocultures, they inhibited the formation of AChR aggregates at and near neuromuscular contacts, demonstrating that agrin plays a role in the induction of the aggregates. Rat motor neurons, like chick motor neurons, induce AChR aggregates on chick myotubes. This effect was not inhibited by our antibodies, indicating that, although the antibodies inhibited the activity of chick agrin, they did not have a similar effect on rat agrin. We conclude that agrin released by rat motor neurons induced the chick myotubes to aggregate AChRs.
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“Neuronal-specific Agrn isoforms containing the Z exons (32 and 33) are the most potent in promoting AChR aggregation74–77.”
From The neurogenetics of alternative splicing (2016) · cites this paper as [75]
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Metadata
- Authors
- Reist, N. E., Werle, M. J., & McMahan, U. J.
- Affiliations
- Stanford Medicine (US)
- ORCID
- Reist, Noreen E.
- Venue
- Neuron
- Publisher
- Elsevier BV
- Published
- 1992-05
- Volume
- 8
- Issue
- 5
- Pages
- 865-868
- ISSN
- 0896-6273 (ISSN-L)
- OpenAlex
- W2068836997
- Cited by
- 237
- References
- 21
- Citations by year
- 2026: 3 · 2025: 5 · 2024: 3 · 2023: 2 · 2022: 6 · 2020: 7 · 2019: 5 · 2018: 2 · 2017: 2 · 2016: 5 · 2015: 4 · 2014: 3 · 2013: 7 · 2012: 5
- FWCI
- 8.60
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Neuron (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nicotinic Acetylcholine Receptors Study
- Health Sciences › Medicine › Clinical Neurology › Myasthenia Gravis and Thymoma
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Keywords
- agrin, acetylcholine receptor aggregation, neuromuscular junction, motor neurons, polyclonal antibodies, skeletal muscle
- MeSH terms
- Animals, Antibodies, Cells, Cultured, Chickens, Motor Neurons, Nerve Tissue Proteins, Neuromuscular Junction, Receptors, Cholinergic, Agrin, Macromolecular Substances, Rats
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