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Distribution of 17β-hydroxysteroid dehydrogenase gene expression and activity in rat and human tissues.
Abstract
The interconversion of estrone (E1) and 17 beta-estradiol (E2), androstenedione (4-ene-dione) and testosterone (T), as well as dehydroepiandrosterone and androst-5-ene-3 beta,17 beta-diol is catalyzed by 17 beta-hydroxysteroid dehydrogenase (17 beta-HSD). The enzyme 17 beta-HSD thus plays an essential role in the formation of all active androgens and estrogens in gonadal as well as extragonadal tissues. The present study investigates the tissue distribution of 17 beta-HSD activity in the male and female rat as well as in some human tissues and the distribution of 17 beta-HSD mRNA in some human tissues. Enzymatic activity was measured using 14C-labeled E1, E2, 4-ene-dione and T as substrates. Such enzymatic activity was demonstrated in all 17 rat tissues examined for both androgenic and estrogenic substrates. While the liver had the highest level of 17 beta-HSD activity, low but significant levels of E2 as well as T formation were found in rat brain, heart, pancreas and thymus. The oxidative pathway (E2----E1, T----4-ene-dione) was favored over the reverse reaction in almost all rat tissues while in the human, almost equal rates were found in most of the 15 tissues examined. The widespread distribution of 17 beta-HSD in rat and human tissues clearly indicates the importance of this enzyme in peripheral sex steroid formation or intracrinology.
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“the enzymes required for the formation of androgens and estrogens are expressed in rat peripheral tissues (260,261).”
From Endocrine and intracrine sources of androgens in women: inhibition of breast cancer and other roles of androgens and their precursor dehydroepiandrosterone (2003) · cites this paper as [260]
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Self-citation
Metadata
- Authors
- Martel, C., Rhéaume, E., Takahashi, M., Trudel, C., Couët, J., Luu-The, V., Simard, J., & Labrie, F.
- Affiliations
- Centre hospitalier de l'Université Laval (CA)
- Corresponding author
- Labrie, F.
- Venue
- The Journal of Steroid Biochemistry and Molecular Biology
- Publisher
- Elsevier BV
- Published
- 1992-03
- Volume
- 41
- Issue
- 3-8
- Pages
- 597-603
- ISSN
- 0960-0760 (ISSN-L)
- OpenAlex
- W2014783542
- Cited by
- 231
- References
- 30
- Citations by year
- 2026: 1 · 2025: 3 · 2023: 1 · 2022: 2 · 2021: 3 · 2020: 4 · 2019: 3 · 2018: 3 · 2017: 3 · 2016: 4 · 2015: 2 · 2014: 3 · 2013: 4 · 2012: 3
- FWCI
- 7.78
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- The Journal of Steroid Biochemistry and Molecular Biology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Estrogen and related hormone effects
- Physical Sciences › Chemistry › Organic Chemistry › Steroid Chemistry and Biochemistry
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Hormonal and reproductive studies
- Keywords
- 17β-hydroxysteroid dehydrogenase, tissue distribution, enzyme activity, mRNA expression, estrone, estradiol, androstenedione, testosterone, dehydroepiandrosterone, androgen production, estrogen synthesis, liver, rats, human tissue
- MeSH terms
- Androgens, Animals, Base Sequence, Estrogens, Female, Humans, Male, Molecular Sequence Data, Oligodeoxyribonucleotides, Organ Specificity, Rats, Inbred Strains, Sex Characteristics, Species Specificity, 17-Hydroxysteroid Dehydrogenases, Gene Expression, Polymerase Chain Reaction, Rats
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