Cited by 173Sexual Differentiation and DisordersAdrenal Hormones and DisordersEstrogen and related hormone effects

Structure of two in tandem human 17β-hydroxysteroid dehydrogenase genes

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Luu-The, Van, Labrie, Claude, Simard, Jacques, Lachance, Yves, Zhao, Hui-Fen, Couët, Jacques, Leblanc, Gilles, Labrie, Fernand·1990·Molecular Endocrinology

Abstract

Two human 17 beta-hydroxysteroid dehydrogenase (17 beta-HSD) genes (h17 beta-HSDI and h17 beta-HSDII) included in tandem within an approximately 13 kilobase pair fragment were isolated from a genomic lambda EMBL3 DNA library using cDNA encoding human 17 beta-HSD (hpE2DH216) as probe. We have determined the complete exon and intron sequences of the two genes as well as their 5' and 3'-flanking regions. Human 17 beta-HSDII contains six exons and five short introns for a total length of 3250 base pairs. The exon sequence of h17 beta-HSDII is identical to the previously reported hpE2DH216 cDNA while the overlapping nucleotide sequences of the corresponding exons and introns of h17 beta-HSDI and h17 beta-HSDII show 89% homology. In addition, we have used the hpE2DH216 cDNA to demonstrate the widespread expression of 17 beta-HSD mRNAs in steroidogenic and peripheral target tissues. These new findings provide the basis for a better understanding of the molecular mechanisms involved in 17 beta-HSD deficiency and peripheral sex steroid metabolism.

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Crossref · 10.1210/mend-4-2-268

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  • In the late 1980s, three independent groups reported the cloning of its cDNA, the first of any human HSD.

    “In the late 1980s, three independent groups reported the cloning of its cDNA, the first of any human HSD (503-505).”

    From The Molecular Biology, Biochemistry, and Physiology of Human Steroidogenesis and Its Disorders (2011) · cites this paper as [505]

    The Introduction reports that the authors had previously isolated and characterized human placental 17β-HSD cDNAs, and Cloning and Nucleotide Sequencing describes using one as a probe. Neither passage establishes that three independent groups reported the cloning or that this was the first cDNA cloned for any human HSD. (LLM, conf=0.84)

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Research works printed but not linked: 2

  1. Lacoste D, Belanger A, Labrie F 1989 Biosynthesis and degradation of androgens in human prostatic cancer cell lines. In: Bradlow H, Castagnetta L, d'Aquino S, Labrie F (eds) Steroid Formation, Degradation and Action in Pe- ripheral Normal and Neoplastic Tissues. Ann New York Acad Sci, NY, in press
  2. Greene JM 1987 Analysis of RNA structure and synthesis. In: Ausubel FM, Brent R, Kingston RE, Moore DD, Seid- man TG, Smith TA, Struhl K (eds) Current Protocols in Molecular Biology. Wiley and Sons, New York, pp 4.6.1- 4.6.5

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Authors
Luu-The, V., Labrie, C., Simard, J., Lachance, Y., Zhao, H.-F., Couët, J., Leblanc, G., & Labrie, F.
Affiliations
Université Laval (CA)
Venue
Molecular Endocrinology
Published
1990-02
Volume
4
Issue
2
Pages
268-275
ISSN
  • 0888-8809 (ISSN-L)
  • 1944-9917
OpenAlex
W2074285202
Cited by
173
References
27
Citations by year
2024: 1 · 2018: 1 · 2017: 2 · 2015: 5 · 2014: 1
FWCI
5.75
Type
journal article
Language
en
Open access
Locations
  • Molecular Endocrinology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Sexual Differentiation and Disorders
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adrenal Hormones and Disorders
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
Keywords
17β-hydroxysteroid dehydrogenase, tandem gene duplication, gene structure, exon-intron organization, 5' and 3' flanking regions, steroidogenic tissues, mRNA expression
MeSH terms
Amino Acid Sequence, Base Sequence, Chromosome Mapping, Genes, Humans, Molecular Sequence Data, RNA, Messenger, 17-Hydroxysteroid Dehydrogenases, Restriction Mapping, DNA Probes, Genomic Library

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