Assessment of lipid peroxidation by measuring malondialdehyde (MDA) and relatives in biological samples: Analytical and biological challenges
Abstract
Malondialdehyde (MDA), 4-hydroxy-nonenal (HNE) and the F 2 -isoprostane 15(S)-8-iso-prostaglandin F 2α (15(S)-8-iso-PGF 2α ) are the best investigated products of lipid peroxidation. MDA, HNE and 15(S)-8-iso-PGF 2α are produced from polyunsaturated fatty acids (PUFAs) both by chemical reactions and by reactions catalyzed by enzymes. 15(S)-8-iso-PGF 2α and other F 2 -isoprostanes are derived exclusively from arachidonic acid (AA). The number of PUFAs that may contribute to MDA and HNE is much higher. MDA is the prototype of the so called thiobarbituric acid reactive substances (TBARS). MDA, HNE and 15(S)-8-iso-PGF 2α are the most frequently measured biomarkers of oxidative stress, namely of lipid peroxidation. In many diseases, higher concentrations of MDA, HNE and 15(S)-8-iso-PGF 2α are measured in biological samples as compared to health. Therefore, elevated oxidative stress is generally regarded as a pathological condition. Decreasing the concentration of biomarkers of oxidative stress by changing life style, by nutritional intake of antioxidants or by means of drugs is generally believed to be beneficial to health. Reliable assessment of oxidative stress by measuring MDA, HNE and 15(S)-8-iso-PGF 2α in biological fluids is highly challenging for two important reasons: Because of the duality of oxidative stress, i.e., its origin from chemical and enzymatic reactions, and because of pre-analytical and analytical issues. This article focuses on these key issues. It reviews rep…
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Lipid peroxidation has been implicated in a high number of human pathologic conditions.
“The biological process of lipid peroxidation has been implicated in a high number of human pathologic conditions [172,173].”
From Protein oxidation - Formation mechanisms, detection and relevance as biomarkers in human diseases (2021) · cites this paper as [172]
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Protein oxidation - Formation mechanisms, detection and relevance as biomarkers in human diseases
Kehm, R., Baldensperger, T., Raupbach, J., & Höhn, A. (2021) · Redox Biology · doi:10.1016/j.redox.2021.101901 · Cited by 348 · see citing works
“The biological process of lipid peroxidation has been implicated in a high number of human pathologic conditions [172,173].”
BackgroundChemistry and analysis of HNE and other prominent carbonyl-containing lipid oxidation compounds
Sousa, B. C., Pitt, A. R., & Spickett, C. M. (2017) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2017.02.003 · Cited by 172 · see citing works
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Walnut Oil Prevents Scopolamine-Induced Memory Dysfunction in a Mouse Model
Liao, J., Nai, Y., Feng, L., Chen, Y., Li, M., & Xu, H. (2020) · Molecules · doi:10.3390/molecules25071630 · Cited by 53 · see citing works
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Blood-Based Oxidation Markers in Medicated and Unmedicated Schizophrenia Patients: A Meta-Analysis
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- Authors
- Tsikas, D.
- Affiliations
- Medizinische Hochschule Hannover (DE)
- Corresponding author
- Tsikas, Dimitrios
- ORCID
- Tsikas, Dimitrios
- Venue
- Analytical Biochemistry
- Publisher
- Elsevier BV
- Published
- 2017-05
- Volume
- 524
- Pages
- 13-30
- ISSN
- 0003-2697 (ISSN-L)
- OpenAlex
- W2538132594
- Cited by
- 2,190
- References
- 1
- Citations by year
- 2026: 260 · 2025: 374 · 2024: 338 · 2023: 314 · 2022: 324 · 2021: 224 · 2020: 177 · 2019: 114 · 2018: 41 · 2017: 21 · 2014: 1
- FWCI
- 5.47
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Analytical Biochemistry (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
- Keywords
- lipid peroxidation, malondialdehyde, 4-hydroxynonenal, F2-isoprostanes, oxidative stress biomarkers, TBARS, polyunsaturated fatty acids, arachidonic acid, pre-analytical variables, plasma, urine
- MeSH terms
- Animals, Fatty Acids, Unsaturated, Humans, Malondialdehyde, Lipid Peroxidation, Thiobarbituric Acid Reactive Substances, Oxidative Stress
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