Stepwise solubilization-based antigen removal for xenogeneic scaffold generation in tissue engineering
Abstract
The ability of residual antigens on decellularized tissue to elicit the immune response upon implantation motivates development of a more rigorous antigen removal (AR) process for xenogeneic scaffold generation. Antigen removal strategies promoting solubilization of hydrophilic proteins (predominantly cytoplasmic) enhance the reduction of hydrophilic antigenicity in bovine pericardium (BP); however, the diversity of protein antigens within a tissue necessitates development of AR strategies capable of addressing a spectrum of protein antigen solubilities. In the present study, methods for promoting solubilization of lipophilic proteins (predominantly membrane) were investigated for their ability to reduce lipophilic antigenicity of BP when applied as a second AR step following our previously described hydrophilic AR method. Bovine pericardium following AR (BP-AR) was assessed for residual hydrophilic and lipophilic antigenicity, removal of known lipophilic xenoantigens, tensile properties, and extracellular matrix structure and composition. Facilitating hydrophile solubilization (using dithiothreitol and potassium chloride) followed by lipophile solubilization (using amidosulfobetaine-14 (ASB-14)), in a two-step sequential, differential AR strategy, significantly reduces residual hydrophilic and lipophilic antigenicity of BP-AR beyond that achieved with either one-step hydrophilic AR or decellularization using 1% (w/v) sodium dodecyl sulfate. Moreover, use of 1% (w/v) ASB-14 for lipophilic AR eliminates the two most critical known barriers to xenotransplantation (galactose-α(1,3)-galactose and major histocompatibility complex I)) from BP-AR without compromising the structure-function properties of the biomaterial. This study demonstrates the importance of a sequential, differential protein solubilization approach to reduce biomaterial antigenicity in the production of a xenogeneic scaffold for heart valve tissue engineering.
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Sequential solubilization employs salts and reducing agents, and amidosulfobetaines to remove hydrophilic and hydrophobic antigens, respectively.
“Sequential solubilization employs salts and reducing agents, and amidosulfobetaines to remove hydrophilic and hydrophobic, respectively [46, 187].”
From Immunogenicity of decellularized extracellular matrix scaffolds: a bottleneck in tissue engineering and regenerative medicine (2023) · cites this paper as [187]
Acellular scaffolds have a variety of known and unknown protein antigens with heterogeneous solubility, classified into hydrophilic and hydrophobic.
“Acellular scaffolds have a variety of known and unknown protein antigens with heterogeneous solubility, classified into hydrophilic and hydrophobic [187].”
From Immunogenicity of decellularized extracellular matrix scaffolds: a bottleneck in tissue engineering and regenerative medicine (2023) · cites this paper as [187]
Sequential solubilization is an efficient antigen removal method that can be applied following cell removal to augment efficacy in decreasing antigenicity of derived dECM scaffolds.
“Sequential solubilization is an efficient antigen removal method that could be applied following the cell removal process to efficiently augment its efficacy in decreasing the antigenicity of derived dECM scaffolds.”
From Immunogenicity of decellularized extracellular matrix scaffolds: a bottleneck in tissue engineering and regenerative medicine (2023) · cites this paper as [187]
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Self-citationTEMPOROMANDİBULAR EKLEM TAMİR VE REJENERASYONUNDA DOKU MÜHENDİSLİĞİ UYGULAMALARI
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Development of sterilisation strategies for decellularised peripheral nerve grafts
Holland, J. D. R. (2019) · White Rose eTheses Online (University of Leeds, The University of Sheffield, University of York)
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Self-citationWang, R. M., Duran, P., & Christman, K. L. (2020) · Elsevier eBooks · doi:10.1016/b978-0-12-816137-1.00027-1
Shortreed, N. A., & Griffiths, L. G. (2026) · Biomaterials · doi:10.1016/j.biomaterials.2026.124134
Self-citationAntigen Removal Approach to Murine Larynx Engineering Optimizes Extracellular Matrix Preservation
Hardy, E. G., Myers, C. E., Richards, A. M., Yeleswarapu, S., Skinner, J., Griffiths, L., & Lott, D. G. (2026) · The Laryngoscope · doi:10.1002/lary.70678
Self-citationZhang, Z., Deng, X., Liu, S., Guo, Q., & Qin, L. (2026) · Journal of orthopaedic surgery · doi:10.1177/10225536261494292
- Authors
- Wong, M. L., Wong, J. L., Athanasiou, K. A., & Griffiths, L. G.
- Affiliations
- University of California, Davis (US)
- Corresponding author
- Griffiths, Leigh G.
- Venue
- Acta Biomaterialia
- Publisher
- Elsevier BV
- Published
- 2013-05
- Volume
- 9
- Issue
- 5
- Pages
- 6492-6501
- ISSN
- 1742-7061 (ISSN-L)
- OpenAlex
- W2054800475
- Cited by
- 65
- References
- 38
- Citations by year
- 2026: 3 · 2024: 3 · 2023: 5 · 2022: 2 · 2021: 4 · 2020: 5 · 2019: 6 · 2018: 10 · 2017: 5 · 2016: 8 · 2015: 8 · 2014: 4 · 2013: 2
- FWCI
- 6.68
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Acta Biomaterialia (publishedVersion)
- PubMed (publishedVersion)
- Funders
- American Heart Association (11SDG4980023)
- Topics
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Health Sciences › Medicine › Surgery › Xenotransplantation and immune response
- Physical Sciences › Materials Science › Biomaterials › Collagen: Extraction and Characterization
- Keywords
- antigen removal, decellularized tissue, bovine pericardium, protein solubilization, dithiothreitol, sodium dodecyl sulfate, galactose-alpha-1,3-galactose, MHC class I, heart valve tissue engineering, xenotransplantation, tensile properties
- MeSH terms
- Animals, Cattle, Solubility, Tissue Engineering, Tissue Scaffolds
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