CoChat
Sign Up
Cited by 652Hydrogels: synthesis, properties, applicationsOsteoarthritis Treatment and Mechanisms3D Printing in Biomedical Research

Cell-laden hydrogels for osteochondral and cartilage tissue engineering

Open access
Yang, Jingzhou, Zhang, Yu Shrike, Yue, Kan, Khademhosseini, Ali·2017·Acta Biomaterialia
DOI 10.1016/j.actbio.2017.01.036PMC PMC5545789
Open this paper in CoChat — free account

Abstract

Despite tremendous advances in the field of regenerative medicine, it still remains challenging to repair the osteochondral interface and full-thickness articular cartilage defects. This inefficiency largely originates from the lack of appropriate tissue-engineered artificial matrices that can replace the damaged regions and promote tissue regeneration. Hydrogels are emerging as a promising class of biomaterials for both soft and hard tissue regeneration. Many critical properties of hydrogels, such as mechanical stiffness, elasticity, water content, bioactivity, and degradation, can be rationally designed and conveniently tuned by proper selection of the material and chemistry. Particularly, advances in the development of cell-laden hydrogels have opened up new possibilities for cell therapy. In this article, we describe the problems encountered in this field and review recent progress in designing cell-hydrogel hybrid constructs for promoting the reestablishment of osteochondral/cartilage tissues. Our focus centers on the effects of hydrogel type, cell type, and growth factor delivery on achieving efficient chondrogenesis and osteogenesis. We give our perspective on developing next-generation matrices with improved physical and biological properties for osteochondral/cartilage tissue engineering. We also highlight recent advances in biomanufacturing technologies (e.g. molding, bioprinting, and assembly) for fabrication of hydrogel-based osteochondral and cartilage constructs with complex compositions and microarchitectures to mimic their native counterparts. Statement of significance Despite tremendous advances in the field of regenerative medicine, it still remains challenging to repair the osteochondral interface and full-thickness articular cartilage defects. This inefficiency largely originates from the lack of appropriate tissue-engineered biomaterials that replace the damaged regions and promote tissue regeneration. Cell-laden hydrogel systems have emerged as a promising tissue-engineering platform to address this issue. In this article, we describe the fundamental problems encountered in this field and review recent progress in designing cell-hydrogel constructs for promoting the reestablishment of osteochondral/cartilage tissues. Our focus centers on the effects of hydrogel composition, cell type, and growth factor delivery on achieving efficient chondrogenesis and osteogenesis. We give our perspective on developing next-generation hydrogel/inorganic particle/stem cell hybrid composites with improved physical and biological properties for osteochondral/cartilage tissue engineering. We also highlight recent advances in biomanufacturing and bioengineering technologies (e.g. 3D bioprinting) for fabrication of hydrogel-based osteochondral and cartilage constructs.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1016/j.actbio.2017.01.036

Cites

6 of 6 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Hydrogels made from natural polymers, such as polysaccharides (sodium alginate, gelatin, chitosan, hyaluronic acid, and proteins), exhibit good biocompatibility and bioresorbability.

    “Additionally, hydrogels made from natural polymers, such as polysaccharides (sodium alginate, gelatin, chitosan, hyaluronic acid, and proteins), exhibit good biocompatibility and bioresorbability [115,116].”

    From Resorbable Biomaterials Used for 3D Scaffolds in Tissue Engineering: A Review (2023) · cites this paper as [116]

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

Research works printed but not linked: 292

  1. [1]Huey DJ Hu JC Athanasiou KA Unlike bone, cartilage regeneration remains elusive Science 338 6109 2012 917 921 23161992 PMC4327988
  2. [2]Chen H Sun J Hoemann CD Lascau-Coman V Ouyang W McKee MD Shive MS Buschmann MD Drilling and microfracture lead to different bone structure and necrosis during bone-marrow stimulation for cartilage repair J Orthop Res 27 11 2009 1432 1438 19402150
  3. [3]Felson DT Lawrence RC Dieppe PA Hirsch R Helmick CG Jordan JM Kington RS Lane NE Nevitt MC Zhang Y Sowers M McAlindon T Spector TD Poole AR Yanovski SZ Ateshian G Sharma L Buckwalter JA Brandt KD Fries JF Osteoarthritis: new insights. Part 1: the disease and its risk factors Ann Intern Med 133 8 2000 635 646 11033593
  4. [4]C. Centers for Disease, Prevention National and state medical expenditures and lost earnings attributable to arthritis and other rheumatic conditions–United States, 2003 MMWR Morb Mortal Wkly Rep 56 1 2007 4 7 17218935
  5. [5]Felson DT Zhang Y An update on the epidemiology of knee and hip osteoarthritis with a view to prevention Arthri Rheum 41 8 1998 1343 1355 9704632
  6. [6]Mithoefer K McAdams T Williams RJ Kreuz PC Mandelbaum BR Clinical efficacy of the microfracture technique for articular cartilage repair in the knee: an evidence-based systematic analysis Am J Sports Med 37 10 2009 2053 2063 19251676
  7. [7]Mithoefer K Williams RJ 3rd Warren RF Potter HG Spock CR Jones EC Wickiewicz TL Marx RG The microfracture technique for the treatment of articular cartilage lesions in the knee. A prospective cohort study J Bone Joint Surg Am 87 9 2005 1911 1920 16140804
  8. [8]Dorotka R Windberger U Macfelda K Bindreiter U Toma C Nehrer S Repair of articular cartilage defects treated by microfracture and a three-dimensional collagen matrix Biomaterials 26 17 2005 3617 3629 15621252
  9. [9]Harris JD Siston RA Pan X Flanigan DC Autologous chondrocyte implantation: a systematic review J Bone Joint Surg Am 92 12 2010 2220 2233 20844166 PMC7373451
  10. [10]Peterson L Vasiliadis HS Brittberg M Lindahl A Autologous chondrocyte implantation: a long-term follow-up Am J Sports Med 38 6 2010 1117 24 20181804
  11. [11]Selmi TA Verdonk P Chambat P Dubrana F Potel JF Barnouin L Neyret P Autologous chondrocyte implantation in a novel alginate-agarose hydrogel: outcome at two years J Bone Joint Surg Br 90 5 2008 597 604 18450625
  12. [12]Niemeyer P Pestka JM Kreuz PC Erggelet C Schmal H Suedkamp NP Steinwachs M Characteristic complications after autologous chondrocyte implantation for cartilage defects of the knee joint Am J Sports Med 36 11 2008 2091 2099 18801942
  13. [13]Glenn RE Jr McCarty EC Potter HG Juliao SF Gordon JD Spindler KP Comparison of fresh osteochondral autografts and allografts: a canine model Am J Sports Med 34 7 2006 1084 1093 16452265
  14. [14]Benazzo F Cadossi M Cavani F Fini M Giavaresi G Setti S Cadossi R Giardino R Cartilage repair with osteochondral autografts in sheep: effect of biophysical stimulation with pulsed electromagnetic fields J Orthop Res 26 5 2008 631 642 18176941
  15. [15]Raikin SM Fresh osteochondral allografts for large-volume cystic osteochondral defects of the talus J Bone Joint Surg Am 91 12 2009 2818 2826 19952243
  16. [16]Haene R Qamirani E Story RA Pinsker E Daniels TR Intermediate outcomes of fresh talar osteochondral allografts for treatment of large osteochondral lesions of the talus J Bone Joint Surg Am 94 12 2012 1105 1110 22717829
  17. [17]Galperin A Oldinski RA Florczyk SJ Bryers JD Zhang M Ratner BD Integrated bi-layered scaffold for osteochondral tissue engineering Adv Healthc Mater 2 6 2013 872 883 23225568 PMC3644393
  18. [18]Becher C Thermann H Results of microfracture in the treatment of articular cartilage defects of the talus Foot Ankle Int 26 8 2005 583 589 16115412
  19. [19]Kreuz PC Steinwachs MR Erggelet C Krause SJ Konrad G Uhl M Sudkamp N Results after microfracture of full-thickness chondral defects in different compartments in the knee Osteoarthr Cartil 14 11 2006 1119 1125 16815714
  20. [20]Tuan RS A second-generation autologous chondrocyte implantation approach to the treatment of focal articular cartilage defects Arthri Res Ther 9 5 2007 109 PMC2212558 18021426
  21. [21]Giannoni P Pagano A Maggi E Arbico R Randazzo N Grandizio M Cancedda R Dozin B Autologous chondrocyte implantation (ACI) for aged patients: development of the proper cell expansion conditions for possible therapeutic applications Osteoarthr Cartil 13 7 2005 589 600 15979011
  22. [22]Buckwalter JA Articular cartilage injuries Clin Orthop Relat Res 402 2002 21 37 12218470
  23. [23]Bal BS Rahaman MN Jayabalan P Kuroki K Cockrell MK Yao JQ Cook JL In vivo outcomes of tissue-engineered osteochondral grafts J Biomed Mater Res B Appl Biomater 93 1 2010 164 174 20091911
  24. [24]Moutos FT Freed LE Guilak F A biomimetic three-dimensional woven composite scaffold for functional tissue engineering of cartilage Nat Mater 6 2 2007 162 167 17237789
  25. [27]Nuernberger S Cyran N Albrecht C Redl H Vecsei V Marlovits S The influence of scaffold architecture on chondrocyte distribution and behavior in matrix-associated chondrocyte transplantation grafts Biomaterials 32 4 2011 1032 1040 21074264
  26. [28]Dehne T Karlsson C Ringe J Sittinger M Lindahl A Chondrogenic differentiation potential of osteoarthritic chondrocytes and their possible use in matrix-associated autologous chondrocyte transplantation Arthritis Res Ther 11 5 2009 R133 R133 19723327 PMC2787268
  27. [29]Amini AA Nair LS Injectable hydrogels for bone and cartilage repair Biomed Mater 7 2 2012 024105 22456837
  28. [30]Spiller KL Maher SA Lowman AM Hydrogels for the repair of articular cartilage defects Tissue Eng Part B Rev 17 4 2011 281 99 21510824 PMC3171151
  29. [31]Tibbitt MW Anseth KS Hydrogels as extracellular matrix mimics for 3D cell culture Biotechnol Bioeng 103 4 2009 655 63 19472329 PMC2997742
  30. [32]Geckil H Xu F Zhang X Moon S Demirci U Engineering hydrogels as extracellular matrix mimics Nanomedicine 5 3 2010 469 484 20394538 PMC2892416
  31. [33]Skardal A Zhang J McCoard L Xu X Oottamasathien S Prestwich GD Photocrosslinkable hyaluronan-gelatin hydrogels for two-step bioprinting Tissue Eng Part A 16 8 2010 2675 2685 20387987 PMC3135254
  32. [34]Park KM Lee SY Joung YK Na JS Lee MC Park KD Thermosensitive chitosan-Pluronic hydrogel as an injectable cell delivery carrier for cartilage regeneration Acta Biomater 5 6 2009 1956 1965 19261553
  33. [35]Kwon JS Yoon SM Kwon DY Kim DY Tai GZ Jin LM Song B Lee B Kim JH Han DK Min BH Kim MS Injectable in situ-forming hydrogel for cartilage tissue engineering J Mater Chem B 1 26 2013 3314 3321 32261040
  34. [36]Markstedt K Mantas A Tournier I Martinez Avila H Hagg D Gatenholm P 3D Bioprinting Human Chondrocytes with Nanocellulose-Alginate Bioink for Cartilage Tissue Engineering Applications Biomacromolecules 16 5 2015 1489 1496 25806996
  35. [38]Zeng L Yao Y Wang DA Chen X Effect of microcavitary alginate hydrogel with different pore sizes on chondrocyte culture for cartilage tissue engineering Mater Sci Eng C Mater Biol Appl 34 2014 168 175 24268246
  36. [39]Chung JY Song M Ha CW Kim JA Lee CH Park YB Comparison of articular cartilage repair with different hydrogel-human umbilical cord blood-derived mesenchymal stem cell composites in a rat model Stem Cell Res Ther 5 2 2014 39 24646697 PMC4055114
  37. [40]Schutz K Despang F Lode A Gelinsky M Cell-laden biphasic scaffolds with anisotropic structure for the regeneration of osteochondral tissue J Tissue Eng Regen Med 10 5 2016 404 417 24644134
  38. [41]Luo Y Shoichet MS A photolabile hydrogel for guided three-dimensional cell growth and migration Nat Mater 3 4 2004 249 253 15034559
  39. [42]Mauck RL Yuan X Tuan RS Chondrogenic differentiation and functional maturation of bovine mesenchymal stem cells in long-term agarose culture Osteoarthr Cartil 14 2 2006 179 189 16257243
  40. [43]Scholz B Kinzelmann C Benz K Mollenhauer J Wurst H Schlosshauer B Suppression of adverse angiogenesis in an albumin-based hydrogel for articular cartilage and intervertebral disc regeneration Eur Cell Mater 20 2010 24 36 20628970
  41. [44]Oliveira JT Santos TC Martins L Picciochi R Marques AP Castro AG Neves NM Mano JF Reis RL Gellan gum injectable hydrogels for cartilage tissue engineering applications: in vitro studies and preliminary in vivo evaluation Tissue Eng Part A 16 1 2010 343 353 19702512
  42. [45]Yamaoka H Asato H Ogasawara T Nishizawa S Takahashi T Nakatsuka T Koshima I Nakamura K Kawaguchi H Chung UI Takato T Hoshi K Cartilage tissue engineering using human auricular chondrocytes embedded in different hydrogel materials J Biomed Mater Res A 78 1 2006 1 11 16596585
  43. [46]Yuan T Zhang L Li K Fan H Fan Y Liang J Zhang X Collagen hydrogel as an immunomodulatory scaffold in cartilage tissue engineering J Biomed Mater Res B Appl Biomater 102 2 2014 337 344 24000202
  44. [47]Cheng HW Luk KD Cheung KM Chan BP In vitro generation of an osteochondral interface from mesenchymal stem cell-collagen microspheres Biomaterials 32 6 2011 1526 1535 21093047
  45. [48]Chao PH Yodmuang S Wang X Sun L Kaplan DL Vunjak-Novakovic G Silk hydrogel for cartilage tissue engineering J Biomed Mater Res B Appl Biomater 95 1 2010 84 90 20725950 PMC3079331
  46. [49]Eyrich D Brandl F Appel B Wiese H Maier G Wenzel M Staudenmaier R Goepferich A Blunk T Long-term stable fibrin gels for cartilage engineering Biomaterials 28 1 2007 55 65 16962167
  47. [50]Jin R Moreira Teixeira LS Dijkstra PJ Karperien M van Blitterswijk CA Zhong ZY Feijen J Injectable chitosan-based hydrogels for cartilage tissue engineering Biomaterials 30 13 2009 2544 2551 19176242
  48. [51]Choi B Kim S Lin B Wu BM Lee M Cartilaginous extracellular matrix-modified chitosan hydrogels for cartilage tissue engineering ACS Appl Mater Interfaces 6 22 2014 20110 20121 25361212
  49. [52]Mazaki T Shiozaki Y Yamane K Yoshida A Nakamura M Yoshida Y Zhou D Kitajima T Tanaka M Ito Y Ozaki T Matsukawa A A novel, visible light-induced, rapidly cross-linkable gelatin scaffold for osteochondral tissue engineering Sci Rep 4 2014 4457 24662725 PMC3964514
  50. [53]Duan P Pan Z Cao L He Y Wang H Qu Z Dong J Ding J The effects of pore size in bilayered poly(lactide-co-glycolide) scaffolds on restoring osteochondral defects in rabbits J Biomed Mater Res A 102 1 2014 180 192 23637068
  51. [54]Snyder TN Madhavan K Intrator M Dregalla RC Park D A fibrin/hyaluronic acid hydrogel for the delivery of mesenchymal stem cells and potential for articular cartilage repair J Biol Eng 8 2014 10 25061479 PMC4109069
  52. [55]Kim IL Mauck RL Burdick JA Hydrogel design for cartilage tissue engineering: a case study with hyaluronic acid Biomaterials 32 34 2011 8771 8782 21903262 PMC3183132
  53. [56]Mohamad N Mohd Amin MC Pandey M Ahmad N Rajab NF Bacterial cellulose/acrylic acid hydrogel synthesized via electron beam irradiation: accelerated burn wound healing in an animal model Carbohydr Polym 114 2014 312 320 25263896
  54. [57]Park Y Lutolf MP Hubbell JA Hunziker EB Wong M Bovine primary chondrocyte culture in synthetic matrix metalloproteinase-sensitive poly(ethylene glycol)-based hydrogels as a scaffold for cartilage repair Tissue Eng 10 3–4 2004 515 522 15165468
  55. [58]Munoz-Pinto DJ McMahon RE Kanzelberger MA Jimenez-Vergara AC Grunlan MA Hahn MS Inorganic-organic hybrid scaffolds for osteochondral regeneration J Biomed Mater Res A 94 1 2010 112 121 20128006
  56. [59]Zhang W Chen J Tao J Hu C Chen L Zhao H Xu G Heng BC Ouyang HW The promotion of osteochondral repair by combined intra-articular injection of parathyroid hormone-related protein and implantation of a bi-layer collagen-silk scaffold Biomaterials 34 25 2013 6046 6057 23702148
  57. [60]Lai JH Kajiyama G Smith RL Maloney W Yang F Stem cells catalyze cartilage formation by neonatal articular chondrocytes in 3D biomimetic hydrogels Sci Rep 3 2013 3553 24352100 PMC6506564
  58. [61]Nguyen QT Hwang Y Chen AC Varghese S Sah RL Cartilage-like mechanical properties of poly (ethylene glycol)-diacrylate hydrogels Biomaterials 33 28 2012 6682 90 22749448 PMC3572364
  59. [62]Liu SQ Tian Q Hedrick JL Po Hui JH Ee PL Yang YY Biomimetic hydrogels for chondrogenic differentiation of human mesenchymal stem cells to neocartilage Biomaterials 31 28 2010 7298 7307 20615545
  60. [65]Leone G Volpato MD Nelli N Lamponi S Boanini E Bigi A Magnani A Continuous multilayered composite hydrogel as osteochondral substitute J Biomed Mater Res A 103 8 2015 2521 2530 25504681
  61. [66]Zhang L Wang Z Xu C Li Y Gao J Wang W Liu Y High strength graphene oxide/polyvinyl alcohol composite hydrogels Journal of Materials Chemistry 21 28 2011 10399 10406
  62. [68]Kobayashi M Chang YS Oka M A two year in vivo study of polyvinyl alcohol-hydrogel (PVA-H) artificial meniscus Biomaterials 26 16 2005 3243 3248 15603819
  63. [70]Grant C Twigg P Egan A Moody A Smith A Eagland D Crowther N Britland S Poly(vinyl alcohol) hydrogel as a biocompatible viscoelastic mimetic for articular cartilage Biotechnol Prog 22 5 2006 1400 1406 17022680
  64. [71]Guo X Park H Young S Kretlow JD van den Beucken JJ Baggett LS Tabata Y Kasper FK Mikos AG Jansen JA Repair of osteochondral defects with biodegradable hydrogel composites encapsulating marrow mesenchymal stem cells in a rabbit model Acta Biomater 6 1 2010 39 47 19660580 PMC2787824
  65. [72]Holland TA Bodde EW Baggett LS Tabata Y Mikos AG Jansen JA Osteochondral repair in the rabbit model utilizing bilayered, degradable oligo(poly(ethylene glycol) fumarate) hydrogel scaffolds J Biomed Mater Res A 75 1 2005 156 167 16052490
  66. [73]Yasuda K Kitamura N Gong JP Arakaki K Kwon HJ Onodera S Chen YM Kurokawa T Kanaya F Ohmiya Y Osada Y A novel double-network hydrogel induces spontaneous articular cartilage regeneration in vivo in a large osteochondral defect Macromol Biosci 9 4 2009 307 316 19031389
  67. [74]Lee KY Mooney DJ Alginate: Properties and biomedical applications Prog Polym Sci 37 1 2012 106 126 22125349 PMC3223967
  68. [75]Pelletier PHS Payan E Marchal P Choplin L Dellacherie E Amphiphilic derivatives of sodium alginate and hyaluronate for cartilage repair: Rheological properties J Biomed Mater Res A 54 1 2000 102 108 11077408
  69. [76]Hashimoto T Suzuki Y Tanihara M Kakimaru Y Suzuki K Development of alginate wound dressings linked with hybrid peptides derived from laminin and elastin Biomaterials 25 7–8 2004 1407 1414 14643615
  70. [77]Ivanovska J Zehnder T Lennert P Sarker B Boccaccini AR Hartmann A Schneider-Stock R Detsch R Biofabrication of 3D Alginate-Based Hydrogel for Cancer Research: Comparison of Cell Spreading, Viability, and Adhesion Characteristics of Colorectal HCT116 Tumor Cells Tissue Eng Part C Method 22 7 2016 708 715 27269631
  71. [78]Schütz K Placht A-M Paul B Brüggemeier S Gelinsky M Lode A Three-dimensional plotting of a cell-laden alginate/methylcellulose blend: towards biofabrication of tissue engineering constructs with clinically relevant dimensions J Tissue Eng Regen Med 2015 10.1002/term.2058 26202781 · DOI
  72. [80]Ruitong X Zhengyi Z Wenxuan C Yong H Douglas BC Freeform drop-on-demand laser printing of 3D alginate and cellular constructs Biofabrication 7 4 2015 045011 26693735
  73. [81]Tellechea A Silva EA Min J Leal EC Auster ME Pradhan-Nabzdyk L Shih W Mooney DJ Veves A Alginate and DNA Gels Are Suitable Delivery Systems for Diabetic Wound Healing Int J Low Extrem Wounds 14 2 2015 146 153 26032947
  74. [82]Chan G Mooney DJ Ca(2+) released from calcium alginate gels can promote inflammatory responses in vitro and in vivo Acta Biomater 9 12 2013 9281 9291 23938198 PMC3867449
  75. [83]Shea LD Wang D Franceschi RT Mooney DJ Engineered bone development from a pre-osteoblast cell line on three-dimensional scaffolds Tissue Eng 6 6 2000 605 617 11103082
  76. [84]Comisar WA Kazmers NH Mooney DJ Linderman JJ Engineering RGD nanopatterned hydrogels to control preosteoblast behavior: a combined computational and experimental approach Biomaterials 28 30 2007 4409 4417 17619056
  77. [85]Fonseca KB Gomes DB Lee K Santos SG Sousa A Silva EA Mooney DJ Granja PL Barrias CC Injectable MMP-sensitive alginate hydrogels as hMSC delivery systems Biomacromolecules 15 1 2014 380 390 24345197 PMC3918418
  78. [86]Verbeke CS Mooney DJ Injectable, Pore-Forming Hydrogels for In Vivo Enrichment of Immature Dendritic Cells Adv Healthc Mater 4 17 2015 2677 2687 26474318 PMC4715727
  79. [87]Darnell MC Sun JY Mehta M Johnson C Arany PR Suo Z Mooney DJ Performance and biocompatibility of extremely tough alginate/polyacrylamide hydrogels Biomaterials 34 33 2013 8042 8048 23896005 PMC3775708
  80. [88]Desai RM Koshy ST Hilderbrand SA Mooney DJ Joshi NS Versatile click alginate hydrogels crosslinked via tetrazine-norbornene chemistry Biomaterials 50 2015 30 37 25736493
  81. [89]Zeng Q Han Y Li H Chang J Bioglass/alginate composite hydrogel beads as cell carriers for bone regeneration J Biomed Mater Res B Appl Biomater 102 1 2014 42 51 23847006
  82. [90]Vallée F Müller C Durand A Schimchowitsch S Dellacherie E Kelche C Cassel JC Leonard M Synthesis and rheological properties of hydrogels based on amphiphilic alginate-amide derivatives Carbohydr Res 344 2 2009 223 228 19084823
  83. [91]Alsberg E Anderson KW Albeiruti A Franceschi RT Mooney DJ Cell-interactive Alginate Hydrogels for Bone Tissue Engineering J Den Res 80 11 2001 2025 2029 11759015
  84. [92]Sakai S Hirose K Moriyama K Kawakami K Control of cellular adhesiveness in an alginate-based hydrogel by varying peroxidase and H2O2 concentrations during gelation Acta Biomaterialia 6 4 2010 1446 1452 19818883
  85. [93]Lee KY Kong HJ Mooney DJ Quantifying Interactions between Cell Receptors and Adhesion Ligand-Modified Polymers in Solution Macromol Biosci 8 2 2008 140 145 17941112
  86. [95]Awad HA Quinn Wickham M Leddy HA Gimble JM Guilak F Chondrogenic differentiation of adipose-derived adult stem cells in agarose, alginate, and gelatin scaffolds Biomaterials 25 16 2004 3211 3222 14980416
  87. [96]Lima EG Tan AR Tai T Bian L Ateshian GA Cook JL Hung CT Physiologic deformational loading does not counteract the catabolic effects of interleukin-1 in long-term culture of chondrocyte-seeded agarose constructs J Biomech 41 15 2008 3253 3259 18823628 PMC2724593
  88. [97]Bian L Fong JV Lima EG Stoker AM Ateshian GA Cook JL Hung CT Dynamic Mechanical Loading Enhances Functional Properties of Tissue-Engineered Cartilage Using Mature Canine Chondrocytes Tissue Eng Part A 16 5 2009 1781 1790 PMC2952125 20028219
  89. [98]Mauck RL Seyhan SL Ateshian GA Hung CT Influence of Seeding Density and Dynamic Deformational Loading on the Developing Structure/Function Relationships of Chondrocyte-Seeded Agarose Hydrogels Ann Biomed Eng 30 8 2002 1046 1056 12449765
  90. [99]Kelly T-AN Ng KW Wang CCB Ateshian GA Hung CT Spatial and temporal development of chondrocyte-seeded agarose constructs in free-swelling and dynamically loaded cultures J Biomech 39 8 2006 1489 1497 15990101
  91. [100]Román J Cabañas MV Peña J Doadrio JC Vallet-Regí M An optimized β-tricalcium phosphate and agarose scaffold fabrication technique J Biomed Mater Res A 84A 1 2008 99 107 17600331
  92. [101]Sheehy EJ Mesallati T Vinardell T Kelly DJ Engineering cartilage or endochondral bone: A comparison of different naturally derived hydrogels Acta Biomater 13 2015 245 253 25463500
  93. [102]Sheehy EJ Buckley CT Kelly DJ Chondrocytes and bone marrow-derived mesenchymal stem cells undergoing chondrogenesis in agarose hydrogels of solid and channelled architectures respond differentially to dynamic culture conditions J Tissue Eng and Regen Med 5 9 2011 747 758 21953872
  94. [103]Charles Huang CY Reuben PM D’Ippolito G Schiller PC Cheung HS Chondrogenesis of human bone marrow-derived mesenchymal stem cells in agarose culture Anat Rec A Discov Mol Cell Evol Bio 278A 1 2004 428 436 15103737
  95. [104]Erickson IE Huang AH Chung C Li RT Burdick JA Mauck RL Differential Maturation and Structure–Function Relationships in Mesenchymal Stem Cell- and Chondrocyte-Seeded Hydrogels Tissue Eng Part A 15 5 2009 1041 1052 19119920 PMC2810411
  96. [105]Watanabe J Kashii M Hirao M Oka K Sugamoto K Yoshikawa H Akashi M Quick-forming hydroxyapatite/agarose gel composites induce bone regeneration J Biomed Mater Res A 83 3 2007 845 852 17559128
  97. [106]Puértolas JA Vadillo JL Sánchez-Salcedo S Nieto A Gómez-Barrena E Vallet-Regí M Compression behaviour of biphasic calcium phosphate and biphasic calcium phosphate–agarose scaffolds for bone regeneration Acta Biomater 7 2 2011 841 847 20709633
  98. [107]Forget A Christensen J Lüdeke S Kohler E Tobias S Matloubi M Thomann R Shastri VP Polysaccharide hydrogels with tunable stiffness and provasculogenic properties via α-helix to β-sheet switch in secondary structure PNAS 110 32 2013 12887 12892 23886665 PMC3740890
  99. [110]Kim I-Y Seo S-J Moon H-S Yoo M-K Park I-Y Kim B-C Cho C-S Chitosan and its derivatives for tissue engineering applications Biotechnol Adv 26 1 2008 1 21 17884325
  100. [111]Riva R Ragelle H des Rieux A Duhem N Jérôme C Préat V Chitosan and Chitosan Derivatives in Drug Delivery and Tissue Engineering Jayakumar R Prabaharan M Muzzarelli ARA Chitosan for Biomaterials II Springer Berlin Heidelberg Berlin, Heidelberg 2011 19 44
  101. [112]Yamane S Iwasaki N Majima T Funakoshi T Masuko T Harada K Minami A Monde K Nishimura S-i Feasibility of chitosan-based hyaluronic acid hybrid biomaterial for a novel scaffold in cartilage tissue engineering Biomaterials 26 6 2005 611 619 15282139
  102. [113]Hsu S-h Whu SW Hsieh S-C Tsai C-L Chen DC Tan T-S Evaluation of Chitosan-alginate-hyaluronate Complexes Modified by an RGD-containing Protein as Tissue-engineering Scaffolds for Cartilage Regeneration Artif Organs 28 8 2004 693 703 15270950
  103. [114]Waibel KH Haney B Moore M Whisman B Gomez R Safety of Chitosan Bandages in Shellfish Allergic Patients Military Medicine 176 10 2011 1153 1156 22128651
  104. [115]Liu X Ma L Mao Z Gao C Chitosan-Based Biomaterials for Tissue Repair and Regeneration Jayakumar R Prabaharan M Muzzarelli ARA Chitosan for Biomaterials II Springer Berlin Heidelberg Berlin, Heidelberg 2011 81 127
  105. [116]Mourya VK Inamdar NN Trimethyl chitosan and its applications in drug delivery J Mater Sci Mater Med 20 5 2008 1057 1079 19112609
  106. [117]Masuko T Iwasaki N Yamane S Funakoshi T Majima T Minami A Ohsuga N Ohta T Nishimura S-I Chitosan–RGDSGGC conjugate as a scaffold material for musculoskeletal tissue engineering Biomaterials 26 26 2005 5339 5347 15814132
  107. [118]Donati I Stredanska S Silvestrini G Vetere A Marcon P Marsich E Mozetic P Gamini A Paoletti S Vittur F The aggregation of pig articular chondrocyte and synthesis of extracellular matrix by a lactose-modified chitosan Biomaterials 26 9 2005 987 998 15369687
  108. [119]Colnot C Sidhu SS Poirier F Balmain N Cellular and subcellular distribution of galectin-3 in the epiphyseal cartilage and bone of fetal and neonatal mice Cell Mol Biol (Noisy-le-grand) 45 8 1999 1191 1202 10643968
  109. [121]Knudson CB Hyaluronan and CD44: Strategic players for cell–matrix interactions during chondrogenesis and matrix assembly Birth Defects Res C Embryo Today 69 2 2003 174 196 12955860
  110. [122]Akmal M Singh A Anand A Kesani A Aslam N Goodship A Bentley G The effects of hyaluronic acid on articular chondrocytes J Bone Joint Surg Br 87-B 8 2005 1143 1149 16049255
  111. [123]Chung C Mesa J Randolph MA Yaremchuk M Burdick JA Influence of gel properties on neocartilage formation by auricular chondrocytes photoencapsulated in hyaluronic acid networks J Biomed Mater Res A 77 3 2006 518 525 16482551 PMC2678571
  112. [124]Barbucci R Lamponi S Borzacchiello A Ambrosio L Fini M Torricelli P Giardino R Hyaluronic acid hydrogel in the treatment of osteoarthritis Biomaterials 23 23 2002 4503 4513 12322970
  113. [125]Kang JY Chung CW Sung J-H Park B-S Choi J-Y Lee SJ Choi B-C Shim C-K Chung S-J Kim D-D Novel porous matrix of hyaluronic acid for the three-dimensional culture of chondrocytes Int J Pharm 369 1–2 2009 114 120 19059468
  114. [126]Aulin C Bergman K Jensen-Waern M Hedenqvist P Hilborn J Engstrand T In situ cross-linkable hyaluronan hydrogel enhances chondrogenesis J Tissue Eng Regen Med 5 8 2011 188 196 21394931
  115. [127]Erickson IE Kestle SR Zellars KH Farrell MJ Kim M Burdick JA Mauck RL High mesenchymal stem cell seeding densities in hyaluronic acid hydrogels produce engineered cartilage with native tissue properties Acta Biomater 8 8 2012 3027 3034 22546516 PMC3389207
  116. [128]Erickson IE Kestle SR Zellars KH Dodge GR Burdick JA Mauck RL Improved cartilage repair via in vitro pre-maturation of MSC-seeded hyaluronic acid hydrogels Biomed Mater 7 2 2012 024110 22455999 PMC3358514
  117. [129]Chung C Burdick JA Influence of three-dimensional hyaluronic acid microenvironments on mesenchymal stem cell chondrogenesis Tissue Eng Part A 15 2 2009 243 254 19193129 PMC2678568
  118. [130]Bian L Zhai DY Zhang EC Mauck RL Burdick JA Dynamic compressive loading enhances cartilage matrix synthesis and distribution and suppresses hypertrophy in hMSC-laden hyaluronic acid hydrogels Tissue Eng Part A 18 7–8 2012 715 24 21988555 PMC3313608
  119. [131]Unterman SA Gibson M Lee JH Crist J Chansakul T Yang EC Elisseeff JH Hyaluronic acid-binding scaffold for articular cartilage repair Tissue Eng Part A 18 23–24 2012 2497 2506 22724901 PMC3501122
  120. [132]Bian L Zhai DY Tous E Rai R Mauck RL Burdick JA Enhanced MSC chondrogenesis following delivery of TGF-beta3 from alginate microspheres within hyaluronic acid hydrogels in vitro and in vivo Biomaterials 32 27 2011 6425 6434 21652067 PMC3134110
  121. [133]Toh WS Lee EH Guo XM Chan JK Yeow CH Choo AB Cao T Cartilage repair using hyaluronan hydrogel-encapsulated human embryonic stem cell-derived chondrogenic cells Biomaterials 31 27 2010 6968 6980 20619789
  122. [134]Nettles DL Vail TP Morgan MT Grinstaff MW Setton LA Photocrosslinkable Hyaluronan as a Scaffold for Articular Cartilage Repair Ann Biomed Eng 32 3 2004 391 397 15095813
  123. [136]Fenn SL Oldinski RA Visible light crosslinking of methacrylated hyaluronan hydrogels for injectable tissue repair J Biomed Mater Res B Appl Biomater 104 6 2016 1229 1236 26097172 PMC4684809
  124. [137]Oliveira JT Martins L Picciochi R Malafaya PB Sousa RA Neves NM Mano JF Reis RL Gellan gum: A new biomaterial for cartilage tissue engineering applications J Biomed Mater Res Part A 93 3 2010 852 863 19658177
  125. [139]Coutinho DF Sant SV Shin H Oliveira JT Gomes ME Neves NM Khademhosseini A Reis RL Modified Gellan Gum hydrogels with tunable physical and mechanical properties Biomaterials 31 29 2010 7494 7502 20663552 PMC2933815
  126. [140]Du H Hamilton P Reilly M Ravi N Injectable in situ Physically and Chemically Crosslinkable Gellan Hydrogel Macromol Biosci 12 7 2012 952 961 22707249 PMC6052871
  127. [141]Matsukawa S Huang Z Watanabe T Structural change of polymer chains of gellan monitored by circular dichroism Nishinari K Physical Chemistry and Industrial Application of Gellan Gum Springer Berlin Heidelberg Berlin, Heidelberg 1999 92 97
  128. [142]Nitta Y Takahashi R Nishinari K Viscoelasticity and Phase Separation of Aqueous Na-Type Gellan Solution Biomacromolecules 11 1 2010 187 191 19817431
  129. [143]Oliveira JT Santos TC Martins L Silva MA Marques AP Castro AG Neves NM Reis RL Performance of new gellan gum hydrogels combined with human articular chondrocytes for cartilage regeneration when subcutaneously implanted in nude mice J Tissue Eng Regen Med 3 7 2009 493 500 19598145
  130. [144]Smith AM Shelton R Perrie Y Harris JJ An Initial Evaluation of Gellan Gum as a Material for Tissue Engineering Applications J Biomater Appl 22 3 2007 241 254 17494964
  131. [146]Fan J Gong Y Ren L Varshney RR Cai D Wang DA In vitro engineered cartilage using synovium-derived mesenchymal stem cells with injectable gellan hydrogels Acta Biomater 6 3 2010 1178 1185 19733701
  132. [147]Douglas TEL Krawczyk G Pamula E Declercq HA Schaubroeck D Bucko MM Balcaen L Van Der Voort P Bliznuk V van den Vreken NMF Dash M Detsch R Boccaccini AR Vanhaecke F Cornelissen M Dubruel P Generation of composites for bone tissue-engineering applications consisting of gellan gum hydrogels mineralized with calcium and magnesium phosphate phases by enzymatic means J Tissue Eng Regen Med 10 11 2016 938 954 24616374
  133. [148]Timothy ELD Wojciech P Elzbieta P Jana L David S Sander CGL Gilles B Lieve B Rainer D Heidi D Katarzyna CK Agnieszka D Vincent MJIC Frank V Ria C Tom C Aldo RB Peter D Injectable self-gelling composites for bone tissue engineering based on gellan gum hydrogel enriched with different bioglasses Biomed Mater 9 4 2014 045014 25065649
  134. [149]Gantar A da Silva LP Oliveira JM Marques AP Correlo VM Novak S Reis RL Nanoparticulate bioactive-glass-reinforced gellan-gum hydrogels for bone-tissue engineering Mater Sci Eng C Mater Biol Appl 43 2014 27 36 25175184
  135. [151]Schinagl RM Gurskis D Chen AC Sah RL Depth-dependent confined compression modulus of full-thickness bovine articular cartilage J Orthop Res 15 4 1997 499 506 9379258
  136. [153]Lee H Fisher S Kallos MS Hunter CJ Optimizing gelling parameters of gellan gum for fibrocartilage tissue engineering J Biomed Mater Res B Appl Biomater 98 2 2011 238 245 21604364
  137. [154]Silva-Correia J Gloria A Oliveira MB Mano JF Oliveira JM Ambrosio L Reis RL Rheological and mechanical properties of acellular and cell-laden methacrylated gellan gum hydrogels J Biomed Mater Res A 101 12 2013 3438 3446 23568694
  138. [155]Shin H Olsen BD Khademhosseini A The mechanical properties and cytotoxicity of cell-laden double-network hydrogels based on photocrosslinkable gelatin and gellan gum biomacromolecules Biomaterials 33 11 2012 3143 3152 22265786 PMC3282165
  139. [156]Silva-Correia J Zavan B Vindigni V Silva TH Oliveira JM Abatangelo G Reis RL Biocompatibility Evaluation of Ionic- and Photo-Crosslinked Methacrylated Gellan Gum Hydrogels: In Vitro and In Vivo Study Adv Healthc Mater 2 4 2013 568 575 23184642
  140. [157]Frantz C Stewart KM Weaver VM The extracellular matrix at a glance J Cell Sci 123 24 2010 4195 4200 21123617 PMC2995612
  141. [158]Marquass B Somerson JS Hepp P Aigner T Schwan S Bader A Josten C Zscharnack M Schulz RM A novel MSC-seeded triphasic construct for the repair of osteochondral defects J Orthop Res 28 12 2010 1586 1599 20973061
  142. [159]Pulkkinen HJ Tiitu V Valonen P Jurvelin JS Lammi MJ Kiviranta I Engineering of cartilage in recombinant human type II collagen gel in nude mouse model in vivo Osteoarthr Cartil 18 8 2010 1077 1087 20472086
  143. [160]Ren X Wang F Chen C Gong X Yin L Yang L Engineering zonal cartilage through bioprinting collagen type II hydrogel constructs with biomimetic chondrocyte density gradient BMC Musculoskelet Disord 17 1 2016 1 10 27439428 PMC4955200
  144. [161]Bosnakovski D Mizuno M Kim G Takagi S Okumura M Fujinaga T Chondrogenic differentiation of bovine bone marrow mesenchymal stem cells (MSCs) in different hydrogels: Influence of collagen type II extracellular matrix on MSC chondrogenesis Biotechnol Bioeng 93 6 2006 1152 1163 16470881
  145. [162]Suri S Schmidt CE Photopatterned collagen–hyaluronic acid interpenetrating polymer network hydrogels Acta Biomater 5 7 2009 2385 2397 19446050
  146. [163]Rafat M Li F Fagerholm P Lagali NS Watsky MA Munger R Matsuura T Griffith M PEG-stabilized carbodiimide crosslinked collagen–chitosan hydrogels for corneal tissue engineering Biomaterials 29 29 2008 3960 3972 18639928
  147. [164]Guoping Chen TS Takashi Ushida, Naoyuki Ochiai, Tetsuya Tateishi, Tissue Engineering of Cartilage Using a Hybrid Scaffold of Synthetic Polymer and Collagen Tissue Eng 10 3–4 2004 323 330 15165449
  148. [165]Shinichi Ibusuki AP Ranka Milan P Halbesma Gertjan J Randolph Mark A Redmond Robert W Kochevar Irene E Gill Thomas J Engineering Cartilage in a Photochemically Crosslinked Collagen Gel J Knee Surg 22 1 2009 72 81 19216355
  149. [166]Sakai S Hirose K Taguchi K Ogushi Y Kawakami K An injectable, in situ enzymatically gellable, gelatin derivative for drug delivery and tissue engineering Biomaterials 30 20 2009 3371 3377 19345991
  150. [167]Koshy ST Desai RM Joly P Li J Bagrodia RK Lewin SA Joshi NS Mooney DJ Click-Crosslinked Injectable Gelatin Hydrogels Adv Healthc Mater 5 5 2016 541 547 26806652 PMC4849477
  151. [169]Schuurman W Levett PA Pot MW van Weeren PR Dhert WJA Hutmacher DW Melchels FPW Klein TJ Malda J Gelatin-Methacrylamide Hydrogels as Potential Biomaterials for Fabrication of Tissue-Engineered Cartilage Constructs Macromol Biosci 13 5 2013 551 561 23420700
  152. [170]Vivian HMM Ferry PWM Jetze V Wouter JAD Debby G Jos M Yield stress determines bioprintability of hydrogels based on gelatin-methacryloyl and gellan gum for cartilage bioprinting Biofabrication 8 3 2016 035003 27431733 PMC4954607
  153. [171]Visser J Levett PA te Moller NCR Besems J Boere KWM van Rijen MHP de Grauw JC Dhert WJA van Weeren PR Malda J Crosslinkable Hydrogels Derived from Cartilage, Meniscus, and Tendon Tissue Tissue Eng Part A 21 7–8 2015 1195 1206 25557049 PMC4394887
  154. [172]O KC Ravin AG Levin LS Usala A-L Klitzman B Long- and short-term effects of biological hydrogels on capsule microvascular density around implants in rats J Biomed Mater Res A 58 3 2001 313 318 11319747
  155. [174]Yodmuang S McNamara SL Nover AB Mandal BB Agarwal M Kelly T-AN Chao P-hG Hung C Kaplan DL Vunjak-Novakovic G Silk microfiber-reinforced silk hydrogel composites for functional cartilage tissue repair Acta Biomater 11 2015 27 36 25281788 PMC4256092
  156. [175]Kundu B Rajkhowa R Kundu SC Wang X Silk fibroin biomaterials for tissue regenerations Adv Drug Deliv Rev 65 4 2013 457 470 23137786
  157. [176]Wang X Kluge JA Leisk GG Kaplan DL Sonication-induced gelation of silk fibroin for cell encapsulation Biomaterials 29 8 2008 1054 1064 18031805 PMC2693043
  158. [177]Wu X Hou J Li M Wang J Kaplan DL Lu S Sodium dodecyl sulfate-induced rapid gelation of silk fibroin Acta Biomater 8 6 2012 2185 2192 22406911
  159. [178]Yucel T Cebe P Kaplan DL Vortex-Induced Injectable Silk Fibroin Hydrogels Biophys J 97 7 2009 2044 2050 19804736 PMC2756352
  160. [179]Murphy AR John PS Kaplan DL Modification of silk fibroin using diazonium coupling chemistry and the effects on hMSC proliferation and differentiation Biomaterials 29 19 2008 2829 2838 18417206 PMC2698958
  161. [180]Murphy AR Kaplan DL Biomedical applications of chemically-modified silk fibroin J Mater Chem 19 36 2009 6443 6450 20161439 PMC2790051
  162. [181]Kambe Y Yamamoto K Kojima K Tamada Y Tomita N Effects of RGDS sequence genetically interfused in the silk fibroin light chain protein on chondrocyte adhesion and cartilage synthesis Biomaterials 31 29 2010 7503 7511 20643479
  163. [182]Steinmetz NJ Aisenbrey EA Westbrook KK Qi HJ Bryant SJ Mechanical loading regulates human MSC differentiation in a multi-layer hydrogel for osteochondral tissue engineering Acta Biomater 21 2015 142 153 25900444
  164. [184]Goldshmid R Cohen S Shachaf Y Kupershmit I Sarig-Nadir O Seliktar D Wechsler R Steric Interference of Adhesion Supports In-Vitro Chondrogenesis of Mesenchymal Stem Cells on Hydrogels for Cartilage Repair Sci Rep 5 2015 12607 26411496 PMC4585928
  165. [185]Wang W Li B Yang J Xin L Li Y Yin H Qi Y Jiang Y Ouyang H Gao C The restoration of full-thickness cartilage defects with BMSCs and TGF-beta 1 loaded PLGA/fibrin gel constructs Biomaterials 31 34 2010 8964 8973 20822812
  166. [186]Kim K Lam J Lu S Spicer PP Lueckgen A Tabata Y Wong ME Jansen JA Mikos AG Kasper FK Osteochondral tissue regeneration using a bilayered composite hydrogel with modulating dual growth factor release kinetics in a rabbit model J Control Release 168 2 2013 166 178 23541928 PMC3661728
  167. [187]Park H Temenoff JS Tabata Y Caplan AI Mikos AG Injectable biodegradable hydrogel composites for rabbit marrow mesenchymal stem cell and growth factor delivery for cartilage tissue engineering Biomaterials 28 21 2007 3217 3227 17445882 PMC2964378
  168. [188]Berger J Reist M Mayer JM Felt O Peppas NA Gurny R Structure and interactions in covalently and ionically crosslinked chitosan hydrogels for biomedical applications Eur J Pharm Biopharm 57 1 2004 19 34 14729078
  169. [189]Martens PJ Bryant SJ Anseth KS Tailoring the Degradation of Hydrogels Formed from Multivinyl Poly(ethylene glycol) and Poly(vinyl alcohol) Macromers for Cartilage Tissue Engineering Biomacromolecules 4 2 2003 283 292 12625723
  170. [190]Chung C Beecham M Mauck RL Burdick JA The influence of degradation characteristics of hyaluronic acid hydrogels on in vitro neocartilage formation by mesenchymal stem cells Biomaterials 30 26 2009 4287 4296 19464053 PMC2743291
  171. [191]Danielle ARD Benoit SW Anseth Kristi S Manipulations in Hydrogel Degradation Behavior Enhance Osteoblast Function and Mineralized Tissue Formation Tissue Eng 12 6 2006 1663 1673 16846361
  172. [192]Holloway JL Ma H Rai R Burdick JA Modulating hydrogel crosslink density and degradation to control bone morphogenetic protein delivery and in vivo bone formation J Control Release 191 2014 63 70 24905414 PMC4156908
  173. [193]Bouhadir KH Lee KY Alsberg E Damm KL Anderson KW Mooney DJ Degradation of Partially Oxidized Alginate and Its Potential Application for Tissue Engineering Biotechnol Prog 17 5 2001 945 950 11587588
  174. [194]Roberts JJ Nicodemus GD Greenwald EC Bryant SJ Degradation Improves Tissue Formation in (Un)Loaded Chondrocyte-laden Hydrogels Clin Orthop Relat Res 469 10 2011 2725 2734 21347817 PMC3171547
  175. [195]Mario Ferretti KGM Kobayashi Kenji Defail Alicia J Chu Constance R Controlled in Vivo Degradation of Genipin Crosslinked Polyethylene Glycol Hydrogels within Osteochondral Defects Tissue Eng 12 9 2006 2657 2663 16995799
  176. [196]Ko CY Ku KL Yang SR Lin TY Peng S Peng YS Cheng MH Chu IM In vitro and in vivo co-culture of chondrocytes and bone marrow stem cells in photocrosslinked PCL-PEG-PCL hydrogels enhances cartilage formation J Tissue Eng Regen Med 10 10 2016 485 496 24668937
  177. [197]Ko JY Kim KI Park S Im GI In vitro chondrogenesis and in vivo repair of osteochondral defect with human induced pluripotent stem cells Biomaterials 35 11 2014 3571 3581 24462354
  178. [198]Chen K Ng KS Ravi S Goh JC Toh SL In vitro generation of whole osteochondral constructs using rabbit bone marrow stromal cells, employing a two-chambered co-culture well design J Tissue Eng Regen Med 10 4 2016 294 304 23495238
  179. [199]Wang J Rao S Chu J Shen X Levasseur DN Theunissen TW Orkin SH A protein interaction network for pluripotency of embryonic stem cells Nature 444 7117 2006 364 368 17093407
  180. [200]Okita K Ichisaka T Yamanaka S Generation of germline-competent induced pluripotent stem cells Nature 448 7151 2007 313 7 17554338
  181. [201]Yu J Vodyanik MA Smuga-Otto K Antosiewicz-Bourget J Frane JL Tian S Nie J Jonsdottir GA Ruotti V Stewart R Slukvin II Thomson JA Induced pluripotent stem cell lines derived from human somatic cells Science 318 5858 2007 1917 1920 18029452
  182. [202]Haidar ZS Hamdy RC Tabrizian M Delivery of recombinant bone morphogenetic proteins for bone regeneration and repair. Part A: Current challenges in BMP delivery Biotechnol Lett 31 12 2009 1817 1824 19690804
  183. [203]Zouani OF Chollet C Guillotin B Durrieu MC Differentiation of pre-osteoblast cells on poly(ethylene terephthalate) grafted with RGD and/or BMPs mimetic peptides Biomaterials 31 32 2010 8245 8253 20667411
  184. [204]Bergeron E Marquis ME Chretien I Faucheux N Differentiation of preosteoblasts using a delivery system with BMPs and bioactive glass microspheres J Mater Sci Mater Med 18 2 2007 255 263 17323156
  185. [205]Benoit DS Durney AR Anseth KS Manipulations in hydrogel degradation behavior enhance osteoblast function and mineralized tissue formation Tissue Eng 12 6 2006 1663 1673 16846361
  186. [206]Burdick JA Anseth KS Photoencapsulation of osteoblasts in injectable RGD-modified PEG hydrogels for bone tissue engineering Biomaterials 23 22 2002 4315 4323 12219821
  187. [207]Wei X Yang X Han ZP Qu FF Shao L Shi YF Mesenchymal stem cells: a new trend for cell therapy Acta Pharmacol Sin 34 6 2013 747 754 23736003 PMC4002895
  188. [208]Hass R Kasper C Bohm S Jacobs R Different populations and sources of human mesenchymal stem cells (MSC): A comparison of adult and neonatal tissue-derived MSC Cell Commun Signal 9 2011 12 21569606 PMC3117820
  189. [209]Krampera M Pizzolo G Aprili G Franchini M Mesenchymal stem cells for bone, cartilage, tendon and skeletal muscle repair Bone 39 4 2006 678 683 16765663
  190. [210]Murry CE Keller G Differentiation of embryonic stem cells to clinically relevant populations: lessons from embryonic development Cell 132 4 2008 661 680 18295582
  191. [211]Nakagawa M Koyanagi M Tanabe K Takahashi K Ichisaka T Aoi T Okita K Mochiduki Y Takizawa N Yamanaka S Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts Nat Biotechnol 26 1 2008 101 106 18059259
  192. [213]Xavier JR Thakur T Desai P Jaiswal MK Sears N Cosgriff-Hernandez E Kaunas R Gaharwar AK Bioactive nanoengineered hydrogels for bone tissue engineering: a growth-factor-free approach ACS Nano 9 3 2015 3109 3118 25674809
  193. [214]Lu CH Yeh TS Yeh CL Fang YH Sung LY Lin SY Yen TC Chang YH Hu YC Regenerating cartilages by engineered ASCs: prolonged TGF-beta3/BMP-6 expression improved articular cartilage formation and restored zonal structure Mol Ther 22 1 2014 186 195 PMC3978803 23851345
  194. [215]Hwang NS Varghese S Zhang Z Elisseeff J Chondrogenic differentiation of human embryonic stem cell-derived cells in arginine-glycine-aspartate-modified hydrogels Tissue Eng 12 9 2006 2695 2706 16995803
  195. [216]Lam J Lu S Meretoja VV Tabata Y Mikos AG Kasper FK Generation of osteochondral tissue constructs with chondrogenically and osteogenically predifferentiated mesenchymal stem cells encapsulated in bilayered hydrogels Acta Biomater 10 3 2014 1112 1123 24300948 PMC3924599
  196. [217]Visser J Gawlitta D Benders KE Toma SM Pouran B van Weeren PR Dhert WJ Malda J Endochondral bone formation in gelatin methacrylamide hydrogel with embedded cartilage-derived matrix particles Biomaterials 37 2015 174 182 25453948
  197. [218]Sheehy EJ Vinardell T Buckley CT Kelly DJ Engineering osteochondral constructs through spatial regulation of endochondral ossification Acta Biomater 9 3 2013 5484 5492 23159563
  198. [219]Sheehy EJ Mesallati T Kelly L Vinardell T Buckley CT Kelly DJ Tissue Engineering Whole Bones Through Endochondral Ossification: Regenerating the Distal Phalanx BioRes Open Access 4 1 2015 229 241 26309799 PMC4540120
  199. [220]Linkhart TA Mohan S Baylink DJ Growth factors for bone growth and repair: IGF, TGF beta and BMP Bone 19 1 Suppl 1996 1S 12S 8830994
  200. [221]Roberts AB Frolik CA Anzano MA Sporn MB Transforming growth factors from neoplastic and nonneoplastic tissues Fed Proc 42 9 1983 2621 2626 6303865

200 of 292 shown

Cited by

Sorted by citations

Authors
Yang, J., Zhang, Y. S., Yue, K., & Khademhosseini, A.
Affiliations
Brigham and Women's Hospital (US)
Corresponding author
Khademhosseini, Ali
ORCID
Venue
Acta Biomaterialia
Publisher
Elsevier BV
Published
2017-07
Volume
57
Pages
1-25
ISSN
1742-7061 (ISSN-L)
DOI
10.1016/j.actbio.2017.01.036
PMC
PMC5545789
OpenAlex
W2575229100
Cited by
652
References
6
Citations by year
2026: 34 · 2025: 50 · 2024: 79 · 2023: 110 · 2022: 83 · 2021: 93 · 2020: 64 · 2019: 66 · 2018: 56 · 2017: 16
FWCI
3.52
Type
journal article
Language
en
Open access
Licence
Locations
  • Acta Biomaterialia (acceptedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • National Science Foundation (EFRI-1240443)
  • IMMODGEL (602694)
  • National Institutes of Health (EB012597, AR057837, DE021468, HL099073, AI105024, AR063745)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
  • Health Sciences › Medicine › Rheumatology › Osteoarthritis Treatment and Mechanisms
  • Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
Keywords
cell-laden hydrogels, osteochondral tissue engineering, articular cartilage repair, chondrogenesis, bone formation, growth factor delivery, 3D bioprinting, cartilage defects
MeSH terms
Animals, Cartilage, Humans, Osteogenesis, Hydrogels, Chondrogenesis, Tissue Engineering

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review