Controlled Degradation and Mechanical Behavior of Photopolymerized Hyaluronic Acid Networks
Open accessAbstract
Hyaluronic acid is a natural polysaccharide found abundantly throughout the body with many desirable properties for application as a biomaterial, including scaffolding for tissue engineering. In this work, hyaluronic acid with molecular weights ranging from 50 to 1100 kDa was modified with methacrylic anhydride and photopolymerized into networks with a wide range of physical properties. With macromer concentrations from 2 to 20 wt %, networks exhibited volumetric swelling ratios ranging from approximately 42 to 8, compressive moduli ranging from approximately 2 to over 100 kPa, and degradation times ranging from less than 1 day up to almost 38 days in the presence of 100 U/mL of hyaluronidase. When 3T3-fibroblasts were photoencapsulated in the hydrogels, cells remained viable with low macromer concentrations but decreased sequentially as the macromer concentration increased. Finally, auricular swine chondrocytes produced neocartilage when photoencapsulated in the hyaluronic acid networks. This work presents a next step toward the development of advanced in vivo curable biomaterials.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Among most commonly used natural polymers are hyaluronic acid, gelatin, collagen, and chondroitin sulfate.
“Among the most commonly used natural polymers are hyaluronic acid, gelatin, collagen, and chondroitin sulfate (14, 108).”
From Modeling physiological events in 2D vs. 3D cell culture (2017) · cites this paper as [14]
The Introduction (page unavailable) identifies hyaluronic acid and collagen as materials investigated for cartilage scaffolds, supporting their use as natural biomaterials. It does not identify gelatin as a commonly used material, and mentions chondroitin sulfate as a cartilage component rather than as a commonly used polymer. It also does not establish that the four named materials are among the most commonly used. (LLM, conf=0.84)
Experimental — accuracy not yet measured3D scaffolds and associated cell-encapsulation techniques provide valuable tools for deciphering how ECM affects fate of cells.
“These 3D scaffolds and associated cell-encapsulation techniques provide the valuable tools for helping us decipher how the ECM affects the fate of cells (5, 13, 14, 25-27, 68, 77, 80, 87, 88, 92, 108).”
From Modeling physiological events in 2D vs. 3D cell culture (2017) · cites this paper as [14]
The Materials and Methods describe cell encapsulation in 3D hydrogel networks, and the Results and Discussion report how network properties relate to cell viability and neocartilage formation. This supports the scaffolds-and-encapsulation part of the claim, but the paper does not directly examine how the extracellular matrix affects cell fate; it chiefly examines how the networks affect cells and matrix distribution. Page numbers were not available in our copy. (LLM, conf=0.82)
Experimental — accuracy not yet measuredHyaluronic acid gels (e.g., auto-crosslinked HA in Hyalobarrier) form a viscous coating that gradually breaks down by enzymatic action over days to weeks depending on concentration.
“Hyaluronic acid gels (e.g., auto-crosslinked HA in Hyalobarrier®)—these form a viscous coating that gradually breaks down by enzymatic action over days to weeks depending on the concentration [267].”
From Biomaterials in Postoperative Adhesion Barriers and Uterine Tissue Engineering (2025) · cites this paper as [267]
The Results and Discussion (page unavailable) report that hyaluronidase degrades the studied HA networks and that higher macromer concentration extends degradation time; the Abstract reports times from less than one day to almost 38 days under laboratory conditions. Materials and Methods (page unavailable) identify these as methacrylated, photopolymerized HA gels, not auto-crosslinked Hyalobarrier®. The paper does not establish that Hyalobarrier forms a viscous coating or that the measured degradation times apply to it in use. (LLM, conf=0.89)
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Principles of Polymer Chemistry.
Huggins, M. L. (1954) · Journal of the American Chemical Society · doi:10.1021/ja01639a090 · Cited by 16,657 · see citing works
Principles of polymer chemistry
(1995) · Choice Reviews Online · doi:10.5860/choice.33-2140 · Cited by 15,292 · see citing works
A modified uronic acid carbazole reaction
Bitter, T., & Muir, H. M. (1962) · Analytical Biochemistry · doi:10.1016/0003-2697(62)90095-7 · Cited by 6,239 · see citing works
Fluorometric assay of DNA in cartilage explants using Hoechst 33258
Kim, Y. J., Sah, R. L., Doong, J. H., & Grodzinsky, A. J. (1988) · Analytical Biochemistry · doi:10.1016/0003-2697(88)90532-5 · Cited by 1,357 · see citing works
Functions of hyaluronan in wound repair
Chen, W. Y. J., & Abatangelo, G. (1999) · Wound Repair and Regeneration · doi:10.1046/j.1524-475x.1999.00079.x · Cited by 1,089 · see citing works
Burdick, J. A., & Anseth, K. S. (2002) · Biomaterials · doi:10.1016/s0142-9612(02)00176-x · Cited by 1,008 · see citing works
Self-citationBryant, S. J., Nuttelman, C. R., & Anseth, K. S. (2000) · Journal of Biomaterials Science Polymer Edition · doi:10.1163/156856200743805 · Cited by 775 · see citing works
Semisynthetic resorbable materials from hyaluronan esterification
Campoccia, D. (1998) · Biomaterials · doi:10.1016/s0142-9612(98)00042-8 · Cited by 529 · see citing works
Photoencapsulation of chondrocytes in poly(ethylene oxide)-based semi-interpenetrating networks
Elisseeff, J. H., McIntosh, W., Anseth, K. S., Riley, S. L., Ragan, P. M., & Langer, R. (2000) · Journal of Biomedical Materials Research · doi:10.1002/(sici)1097-4636(200008)51:2 · Cited by 510 · see citing works
Self-citationBryant, S. J., & Anseth, K. S. (2002) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.10319 · Cited by 454 · see citing works
Prestwich, G. D., Marecak, D. M., Marecek, J. F., Vercruysse, K. P., & Ziebell, M. R. (1998) · Journal of Controlled Release · doi:10.1016/s0168-3659(97)00242-3 · Cited by 423 · see citing works
Photopolymerized hyaluronic acid-based hydrogels and interpenetrating networks
Park, Y., Tirelli, N., & Hubbell, J. A. (2002) · Biomaterials · doi:10.1016/s0142-9612(02)00420-9 · Cited by 412 · see citing works
Joint resurfacing using allograft chondrocytes and synthetic biodegradable polymer scaffolds
Freed, L. E., Grande, D. A., Lingbin, Z., Emmanual, J. E., Marquis, J. C., & Langer, R. (1994) · Journal of Biomedical Materials Research · doi:10.1002/jbm.820280808 · Cited by 380 · see citing works
Self-citationHyaluronidase and its substrate hyaluronan: biochemistry, biological activities and therapeutic uses
Menzel, E. J., & Farr, C. V. (1998) · Cancer Letters · doi:10.1016/s0304-3835(98)00195-5 · Cited by 347 · see citing works
Bryant, S. J., & Anseth, K. S. (2001) · Biomaterials · doi:10.1016/s0142-9612(00)00225-8 · Cited by 310 · see citing works
Chu, C. R., Coutts, R. D., Yoshioka, M., Harwood, F. L., Monosov, A. Z., & Amiel, D. (1995) · Journal of Biomedical Materials Research · doi:10.1002/jbm.820290915 · Cited by 286 · see citing works
Photocrosslinkable Hyaluronan as a Scaffold for Articular Cartilage Repair
Nettles, D. L., Vail, T. P., Morgan, M. T., Grinstaff, M. W., & Setton, L. A. (2004) · Annals of Biomedical Engineering · doi:10.1023/b:abme.0000017552.65260.94 · Cited by 231 · see citing works
Hyaluronan‐based polymers in the treatment of osteochondral defects
Solchaga, L. A., Yoo, J. U., Lundberg, M., Dennis, J. E., Huibregtse, B. A., Goldberg, V. M., & Caplan, A. I. (2000) · Journal of Orthopaedic Research® · doi:10.1002/jor.1100180515 · Cited by 227 · see citing works
Elisseeff, J. H., Anseth, K. S., Sims, D. C., McIntosh, W., Randolph, M. A., Yaremchuk, M. J., & Langer, R. (1999) · Plastic & Reconstructive Surgery · doi:10.1097/00006534-199909040-00017 · Cited by 223 · see citing works
Self-citationTissue Engineered Neocartilage Using Plasma Derived Polymer Substrates and Chondrocytes
Sims, D. C., Butler, P. E. M., Cao, Y. L., Casanova, R., Randolph, M. A., Black, A. C., Vacanti, C. A., & Yaremchuk, M. J. (1998) · Plastic & Reconstructive Surgery · doi:10.1097/00006534-199805000-00022 · Cited by 183 · see citing works
Self-citationArticular cartilage repair in rabbits by using suspensions of allogenic chondrocytes in alginate
Fragonas, E., Valente, M., Pozzi-Mucelli, M., Toffanin, R., Rizzo, R., Silvestri, F., & Vittur, F. (2000) · Biomaterials · doi:10.1016/s0142-9612(99)00241-0 · Cited by 182 · see citing works
Hylan gel biomaterial: Dermal and immunologic compatibility
Larsen, N. E., Pollak, C. T., Reiner, K., Leshchiner, E. A., & Balazs, E. A. (1993) · Journal of Biomedical Materials Research · doi:10.1002/jbm.820270903 · Cited by 180 · see citing works
Chondrocytes Embedded in Collagen Gels Maintain Cartilage Phenotype During Long-term Cultures
TOMOATS, U., NATSU, O., SUGUR, U., & KEIR, O. (1984) · Clinical Orthopaedics and Related Research · doi:10.1097/00003086-198406000-00039 · Cited by 177 · see citing works
Paige, K. T., Griffith, L. G., Yaremchuk, M. J., Vacanti, J. P., & Vacanti, C. A. (1995) · Plastic & Reconstructive Surgery · doi:10.1097/00006534-199511000-00024 · Cited by 164 · see citing works
Leach, J. B., Bivens, K. A., Collins, C. N., & Schmidt, C. E. (2004) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.30063 · Cited by 153 · see citing works
In Vitro Prefabrication of Human Cartilage Shapes Using Fibrin Glue and Human Chondrocytes
Ting, V., Sims, C., Brecht, L. E., McCarthy, J. G., Kasabian, A. K., Connelly, P. R., Elisseeff, J. H., Gittes, G. K., & Longaker, M. T. (1998) · Annals of Plastic Surgery · doi:10.1097/00000637-199804000-00016 · Cited by 100 · see citing works
Influence of inflammatory cells and serum on the performance of implantable glucose sensors
Gerritsen, M., Jansen, J. A., Kros, A., Vriezema, D. M., Sommerdijk, N. A. J. M., Nolte, R. J. M., Lutterman, J. A., van Hövell, S. W. F. M., & van der Gaag, A. (2000) · Journal of Biomedical Materials Research · doi:10.1002/1097-4636(200101)54:1 · Cited by 89 · see citing works
A new basic metachromatic dye, 1?9-Dimethyl Methylene Blue
Taylor, K. B., & Jeffree, G. M. (1969) · The Histochemical Journal · doi:10.1007/bf01081408 · Cited by 78 · see citing works
Machelon, V., Mercier‐Nomé, F., Grosse, B., & Lieberherr, M. (1996) · Journal of Cellular Biochemistry · doi:10.1002/(sici)1097-4644(19960616)61:4 · Cited by 52 · see citing works
Flagged first, then by citations
Research works printed but not linked: 1
- [1]Kimura T, Yasui N, Ohsawa S, Ono K. Clin Orthop Rel Res. 1984;186:231–239. [PubMed] [Google Scholar]
Cited by
Sorted by citations
Hydrogels in Biology and Medicine: From Molecular Principles to Bionanotechnology
Peppas, N. A., Hilt, J. Z., Khademhosseini, A., & Langer, R. (2006) · Advanced Materials · doi:10.1002/adma.200501612 · Cited by 4,109 · see citing works
Self-citationHydrogels with tunable stress relaxation regulate stem cell fate and activity
Chaudhuri, O., Gu, L., Klumpers, D., Darnell, M., Bencherif, S. A., Weaver, J. C., Huebsch, N., Lee, H.-P., Lippens, E., Duda, G. N., et al. (2016) · Nature Materials · doi:10.1038/nmat4489 · Cited by 2,443 · see citing works
Hyaluronic Acid Hydrogels for Biomedical Applications
Burdick, J. A., & Prestwich, G. D. (2011) · Advanced Materials · doi:10.1002/adma.201003963 · Cited by 2,150 · see citing works
Self-citationModeling physiological events in 2D vs. 3D cell culture
Duval, K., Grover, H., Han, L.-H., Mou, Y., Pegoraro, A. F., Fredberg, J., & Chen, Z. (2017) · Physiology · doi:10.1152/physiol.00036.2016 · Cited by 1,849 · see citing works
Three-dimensional bioprinting of thick vascularized tissues
Kolesky, D. B., Homan, K. A., Skylar-Scott, M. A., & Lewis, J. A. (2016) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1521342113 · Cited by 1,492 · see citing works
25th Anniversary Article: Rational Design and Applications of Hydrogels in Regenerative Medicine
Annabi, N., Tamayol, A., Uquillas, J. A., Akbari, M., Bertassoni, L. E., Cha, C., Camci‐Unal, G., Dokmeci, M. R., Peppas, N. A., & Khademhosseini, A. (2014) · Advanced Materials · doi:10.1002/adma.201303233 · Cited by 1,409 · see citing works
Cell Encapsulation in Biodegradable Hydrogels for Tissue Engineering Applications
Nicodemus, G. D., & Bryant, S. J. (2008) · Tissue Engineering Part B Reviews · doi:10.1089/ten.teb.2007.0332 · Cited by 1,242 · see citing works
Soft Materials by Design: Unconventional Polymer Networks Give Extreme Properties
Zhao, X., Chen, X., Yuk, H., Lin, S., Liu, X., & Parada, G. A. (2021) · Chemical Reviews · doi:10.1021/acs.chemrev.0c01088 · Cited by 1,170 · see citing works
Khetan, S., Güvendiren, M., Legant, W. R., Cohen, D. M., Chen, C. S., & Burdick, J. A. (2013) · Nature Materials · doi:10.1038/nmat3586 · Cited by 1,171 · see citing works
Self-citationMano, J. F., Silva, G. A., Azevedo, H. S., Malafaya, P. B., Sousa, R. A., Silva, S. S., Boesel, L. F., Oliveira, J. M., Santos, T. C., Marques, A. P., et al. (2007) · Journal of The Royal Society Interface · doi:10.1098/rsif.2007.0220 · Cited by 1,140 · see citing works
Main properties and current applications of some polysaccharides as biomaterials
Rinaudo, M. (2008) · Polymer International · doi:10.1002/pi.2378 · Cited by 1,028 · see citing works
Griffin, D., Weaver, W., Scumpia, P. O., Carlo, D. D., & Segura, T. (2015) · Nature Materials · doi:10.1038/nmat4294 · Cited by 881 · see citing works
Polymeric materials for bone and cartilage repair
Puppi, D., Chiellini, E., Piras, A. M., & Chiellini, E. (2010) · Progress in Polymer Science · doi:10.1016/j.progpolymsci.2010.01.006 · Cited by 913 · see citing works
3D printing of hydrogels: Rational design strategies and emerging biomedical applications
Li, J., Wu, C., Chu, P. K.-H., & Gelinsky, M. (2020) · Materials Science and Engineering R Reports · doi:10.1016/j.mser.2020.100543 · Cited by 867 · see citing works
Hyaluronic acid—Based wound dressings: A review
Graça, M. F. P., Miguel, S. P., Cabral, . S. D., & Correia, I. J. (2020) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2020.116364 · Cited by 843 · see citing works
Engineering biocompatible implant surfaces
Bauer, S., Schmuki, P., von der Mark, K., & Park, J. (2012) · Progress in Materials Science · doi:10.1016/j.pmatsci.2012.09.001 · Cited by 783 · see citing works
Chung, C., & Burdick, J. A. (2008) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2007.08.027 · Cited by 760 · see citing works
Self-citationSchanté, C. E., Zuber, G., Herlin, C., & Vandamme, T. (2011) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2011.03.019 · Cited by 710 · see citing works
Stiffening hydrogels to probe short- and long-term cellular responses to dynamic mechanics
Guvendiren, M., & Burdick, J. A. (2012) · Nature Communications · doi:10.1038/ncomms1792 · Cited by 707 · see citing works
Self-citationGerecht, S., Burdick, J. A., Ferreira, L., Townsend, S. A., Langer, R., & Vunjak‐Novakovic, G. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0703723104 · Cited by 666 · see citing works
Self-citationCell-laden hydrogels for osteochondral and cartilage tissue engineering
Yang, J., Zhang, Y. S., Yue, K., & Khademhosseini, A. (2017) · Acta Biomaterialia · doi:10.1016/j.actbio.2017.01.036 · Cited by 652 · see citing works
3D biofabrication strategies for tissue engineering and regenerative medicine
Bajaj, P., Schweller, R. M., Khademhosseini, A., West, J. L., & Bashir, R. (2014) · Annual Review of Biomedical Engineering · doi:10.1146/annurev-bioeng-071813-105155 · Cited by 632 · see citing works
Review: Photopolymerizable and Degradable Biomaterials for Tissue Engineering Applications
Ifkovits, J. L., & Burdick, J. A. (2007) · Tissue Engineering · doi:10.1089/ten.2007.0093 · Cited by 619 · see citing works
Self-citationAbasalizadeh, F., Moghaddam, S. V., Alizadeh, E., akbari, E., Kashani, E., Fazljou, S. M. B., Torbati, M., & Akbarzadeh, A. (2020) · Journal of Biological Engineering · doi:10.1186/s13036-020-0227-7 · Cited by 618 · see citing works
Chemically Modified Biopolymers for the Formation of Biomedical Hydrogels
Muir, V. G., & Burdick, J. A. (2020) · Chemical Reviews · doi:10.1021/acs.chemrev.0c00923 · Cited by 612 · see citing works
Self-citation3D Printing of Shear-Thinning Hyaluronic Acid Hydrogels with Secondary Cross-Linking
Ouyang, L., Highley, C. B., Rodell, C. B., Sun, W., & Burdick, J. A. (2016) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.6b00158 · Cited by 607 · see citing works
Self-citationHyaluronic acid-based hydrogels: from a natural polysaccharide to complex networks
Xu, X., Jha, A. K., Harrington, D. A., Farach‐Carson, M. C., & Jia, X. (2012) · Soft Matter · doi:10.1039/c2sm06463d · Cited by 601 · see citing works
Self-citationDirected assembly of cell-laden microgels for fabrication of 3D tissue constructs
Du, Y., Lo, E., Ali, S., & Khademhosseini, A. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0801866105 · Cited by 594 · see citing works
Direct 3D bioprinting of prevascularized tissue constructs with complex microarchitecture
Zhu, W. Z., Qu, X., Zhu, J., Ma, X., Patel, S. H., Liu, J., Wang, P., Lai, C. S. E., Gou, M., Xu, Y., et al. (2017) · Biomaterials · doi:10.1016/j.biomaterials.2017.01.042 · Cited by 568 · see citing works
Shear-thinning and self-healing hydrogels as injectable therapeutics and for 3D-printing
Loebel, C., Rodell, C. B., Chen, M. H., & Burdick, J. A. (2017) · Nature Protocols · doi:10.1038/nprot.2017.053 · Cited by 567 · see citing works
Self-citationBioinks and bioprinting technologies to make heterogeneous and biomimetic tissue constructs
Ashammakhi, N., Ahadian, S., Xu, C., Montazerian, H., Ko, H., Nasiri, R., Barros, N., & Khademhosseini, A. (2019) · Materials Today Bio · doi:10.1016/j.mtbio.2019.100008 · Cited by 549 · see citing works
Biopolymer-Based Hydrogels for Cartilage Tissue Engineering
Balakrishnan, B., & Banerjee, R. (2011) · Chemical Reviews · doi:10.1021/cr100123h · Cited by 549 · see citing works
Snetkov, P., Zakharova, K., Morozkina, S. N., Olekhnovich, R. O., & Uspenskaya, M. V. (2020) · Polymers · doi:10.3390/polym12081800 · Cited by 550 · see citing works
Mechanical confinement regulates cartilage matrix formation by chondrocytes
Lee, H.-P., Gu, L., Mooney, D. J., Levenston, M. E., & Chaudhuri, O. (2017) · Nature Materials · doi:10.1038/nmat4993 · Cited by 539 · see citing works
Seidlits, S. K., Khaing, Z. Z., Petersen, R. R., Nickels, J. D., Vanscoy, J. E., Shear, J. B., & Schmidt, C. E. (2010) · Biomaterials · doi:10.1016/j.biomaterials.2010.01.125 · Cited by 528 · see citing works
Hydrogel scaffolds for tissue engineering: the importance of polymer choice
Spicer, C. D. (2019) · Polymer Chemistry · doi:10.1039/c9py01021a · Cited by 530 · see citing works
Fakhari, A., & Berkland, C. J. (2013) · Acta Biomaterialia · doi:10.1016/j.actbio.2013.03.005 · Cited by 523 · see citing works
Costa-Júnior, E. S., Barbosa‐Stancioli, E. F., Mansur, A. A. P., Vasconcelos, W. L., & Mansur, H. S. (2008) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2008.11.015 · Cited by 515 · see citing works
Hydrogel design for cartilage tissue engineering: A case study with hyaluronic acid
Kim, I. L., Mauck, R. L., & Burdick, J. A. (2011) · Biomaterials · doi:10.1016/j.biomaterials.2011.08.073 · Cited by 503 · see citing works
Self-citationEngineered Microenvironments for Controlled Stem Cell Differentiation
Burdick, J. A., & Vunjak-Novakovic, G. (2009) · Tissue Engineering Part A · doi:10.1089/ten.tea.2008.0131 · Cited by 485 · see citing works
Self-citationMondschein, R. J., Kanitkar, A., Williams, C. B., Verbridge, S. S., & Long, T. E. (2017) · Biomaterials · doi:10.1016/j.biomaterials.2017.06.005 · Cited by 477 · see citing works
Chung, C., & Burdick, J. A. (2008) · Tissue Engineering Part A · doi:10.1089/ten.tea.2008.0067 · Cited by 465 · see citing works
Self-citationDuan, B., Kapetanovic, E., Hockaday, L. A., & Butcher, J. T. (2013) · Acta Biomaterialia · doi:10.1016/j.actbio.2013.12.005 · Cited by 437 · see citing works
Diels−Alder Click Cross-Linked Hyaluronic Acid Hydrogels for Tissue Engineering
Nimmo, C. M., Owen, S. C., & Shoichet, M. S. (2011) · Biomacromolecules · doi:10.1021/bm101446k · Cited by 434 · see citing works
Shin, J., Lee, J. S., Lee, C., Park, H., Yang, K., Jin, Y., Ryu, J. H., Hong, K. S., Moon, S., Chung, H., et al. (2015) · Advanced Functional Materials · doi:10.1002/adfm.201500006 · Cited by 432 · see citing works
Spatiotemporal hydrogel biomaterials for regenerative medicine
Brown, T. E., & Anseth, K. S. (2017) · Chemical Society Reviews · doi:10.1039/c7cs00445a · Cited by 428 · see citing works
Hydrogel‐Forming Microneedles: Current Advancements and Future Trends
Turner, J. G., White, L. R., Estrela, P., & Leese, H. S. (2020) · Macromolecular Bioscience · doi:10.1002/mabi.202000307 · Cited by 424 · see citing works
Degradable natural polymer hydrogels for articular cartilage tissue engineering
Zhao, W., Jin, X., Cong, Y., Liu, Y., & Fu, J. (2012) · Journal of Chemical Technology & Biotechnology · doi:10.1002/jctb.3970 · Cited by 422 · see citing works
Fundamentals and Applications of Photo-Cross-Linking in Bioprinting
Lim, K. S., Galarraga, J. H., Cui, X., Lindberg, G. C. J., Burdick, J. A., & Woodfield, T. B. F. (2020) · Chemical Reviews · doi:10.1021/acs.chemrev.9b00812 · Cited by 422 · see citing works
Self-citationHyaluronic acid and its biomedical applications: A review
Dovedytis, M., Liu, Z. J., & Bartlett, S. (2020) · Engineered Regeneration · doi:10.1016/j.engreg.2020.10.001 · Cited by 420 · see citing works
Recent progress of in situ formed gels for biomedical applications
Ko, D. Y., Shinde, U. P., Yeon, B., & Jeong, B. (2012) · Progress in Polymer Science · doi:10.1016/j.progpolymsci.2012.08.002 · Cited by 416 · see citing works
Afewerki, S., Sheikhi, A., Kannan, S., Ahadian, S., & Khademhosseini, A. (2018) · Bioengineering & Translational Medicine · doi:10.1002/btm2.10124 · Cited by 414 · see citing works
Shin, H., Olsen, B. D., & Khademhosseini, A. (2012) · Biomaterials · doi:10.1016/j.biomaterials.2011.12.050 · Cited by 401 · see citing works
Review. Hyaluronan: A Powerful Tissue Engineering Tool
Allison, D. D., & Grande-Allen, K. J. (2006) · Tissue Engineering · doi:10.1089/ten.2006.12.2131 · Cited by 400 · see citing works
Naturally derived materials-based cell and drug delivery systems in skin regeneration
Huang, S., & Fu, X. (2009) · Journal of Controlled Release · doi:10.1016/j.jconrel.2009.10.018 · Cited by 392 · see citing works
Lee, Y., Chung, H. J., Yeo, S., Ahn, C., Lee, H., Messersmith, P. B., & Park, T. G. (2010) · Soft Matter · doi:10.1039/b919944f · Cited by 384 · see citing works
Light-Activated Hydrogel Formation via the Triggered Folding and Self-Assembly of a Designed Peptide
Haines-Butterick, L. A., Rajagopal, K., Ozbas, B., Salick, D. A., Pochan, D. J., & Schneider, J. P. (2005) · Journal of the American Chemical Society · doi:10.1021/ja054719o · Cited by 382 · see citing works
Tissue engineering for articular cartilage repair – the state of the art
Johnstone, B., Alini, M., Cucchiarini, M., Dodge, G. R., Eglin, D., Guilak, F., Madry, H., Mata, Á., Mauck, R. L., Semino, C., et al. (2013) · European Cells and Materials · doi:10.22203/ecm.v025a18 · Cited by 371 · see citing works
Polydopamine Decorated Microneedles with Fe‐MSC‐Derived Nanovesicles Encapsulation for Wound Healing
Ma, W., Zhang, X., Liu, Y., Fan, L., Gan, J., Liu, W., Zhao, Y., & Sun, L. (2022) · Advanced Science · doi:10.1002/advs.202103317 · Cited by 360 · see citing works
Hydrogels with time-dependent material properties enhance cardiomyocyte differentiation in vitro
Young, J. L., & Engler, A. J. (2010) · Biomaterials · doi:10.1016/j.biomaterials.2010.10.020 · Cited by 359 · see citing works
3D bioprinting of methacrylated hyaluronic acid (MeHA) hydrogel with intrinsic osteogenicity
Poldervaart, M. T., Goversen, B., de Ruijter, M., Abbadessa, A., Melchels, F. P. W., Öner, F. C., Dhert, W. J. A., Vermonden, T., & Alblas, J. (2017) · PLoS ONE · doi:10.1371/journal.pone.0177628 · Cited by 359 · see citing works
Utech, S., & Boccaccini, A. R. (2015) · Journal of Materials Science · doi:10.1007/s10853-015-9382-5 · Cited by 358 · see citing works
Hyaluronic acid hydrogels with controlled degradation properties for oriented bone regeneration
Patterson, J., Siew, R., Herring, S. W., Lin, A., Guldberg, R. E., & Stayton, P. S. (2010) · Biomaterials · doi:10.1016/j.biomaterials.2010.05.047 · Cited by 347 · see citing works
Synthesis and Characterization of Hybrid Hyaluronic Acid-Gelatin Hydrogels
Camci‐Unal, G., Cuttica, D. F., Annabi, N., Demarchi, D., & Khademhosseini, A. (2013) · Biomacromolecules · doi:10.1021/bm3019856 · Cited by 345 · see citing works
Cosgrove, B. D., Mui, K. L., Driscoll, T. P., Caliari, S. R., Mehta, K. D., Assoian, R. K., Burdick, J. A., & Mauck, R. L. (2016) · Nature Materials · doi:10.1038/nmat4725 · Cited by 345 · see citing works
Self-citationMicromolding of shape-controlled, harvestable cell-laden hydrogels
Yeh, J., Ling, Y., Karp, J. M., Gantz, J., Chandawarkar, A. A., Eng, G. M., Blumling, J., Langer, R., & Khademhosseini, A. (2006) · Biomaterials · doi:10.1016/j.biomaterials.2006.06.005 · Cited by 339 · see citing works
Self-citationTissue engineering of functional articular cartilage: the current status
Kock, L. M., van Donkelaar, C. C., & Ito, K. (2011) · Cell and Tissue Research · doi:10.1007/s00441-011-1243-1 · Cited by 339 · see citing works
Jin, R., Teixeira, L. M., Krouwels, A., Dijkstra, P. J., van Blitterswijk, C., Karperien, M., & Feijén, J. (2009) · Acta Biomaterialia · doi:10.1016/j.actbio.2009.12.024 · Cited by 319 · see citing works
Influence of the degree of methacrylation on hyaluronic acid hydrogels properties
Bencherif, S. A., Srinivasan, A., Horkay, F., Hollinger, J. O., Matyjaszewski, K. A., & Washburn, N. R. (2008) · Biomaterials · doi:10.1016/j.biomaterials.2007.11.047 · Cited by 315 · see citing works
Iatridis, J. C., Nicoll, S. B., Michalek, A. J., Walter, B. A., & Gupta, M. S. (2013) · The Spine Journal · doi:10.1016/j.spinee.2012.12.002 · Cited by 314 · see citing works
Strategies for Hyaluronic Acid-Based Hydrogel Design in Drug Delivery
Trombino, S., Servidio, C., Curcio, F., & Cassano, R. (2019) · Pharmaceutics · doi:10.3390/pharmaceutics11080407 · Cited by 309 · see citing works
Recent advances in polysaccharide-based self-healing hydrogels for biomedical applications
Yang, Y., Xu, L., Wang, J., Meng, Q., Zhong, S., Gao, Y., & Cui, X. (2022) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2022.119161 · Cited by 309 · see citing works
3D printing of functional microrobots
Li, J., & Pumera, M. (2021) · Chemical Society Reviews · doi:10.1039/d0cs01062f · Cited by 306 · see citing works
Cartilage tissue engineering: its potential and uses
Kuo, C. K., Li, W., Mauck, R. L., & Tuan, R. S. (2005) · Current Opinion in Rheumatology · doi:10.1097/01.bor.0000198005.88568.df · Cited by 299 · see citing works
Lee, F., Chung, J. E., & Kurisawa, M. (2008) · Soft Matter · doi:10.1039/b719557e · Cited by 301 · see citing works
Stop-flow lithography to generate cell-laden microgel particles
Panda, P., Ali, S., Lo, E., Chung, B. G., Hatton, T. A., Khademhosseini, A., & Doyle, P. S. (2008) · Lab on a Chip · doi:10.1039/b804234a · Cited by 298 · see citing works
Ifkovits, J. L., Tous, E., Minakawa, M., Morita, M., Robb, J. D., Koomalsingh, K. J., Gorman, J. H., Gorman, R. C., & Burdick, J. A. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1004097107 · Cited by 296 · see citing works
Self-citationKesti, M., Müller, M., Becher, J., Schnabelrauch, M., D’Este, M., Eglin, D., & Zenobi‐Wong, M. (2014) · Acta Biomaterialia · doi:10.1016/j.actbio.2014.09.033 · Cited by 295 · see citing works
Wang, L. L., Highley, C. B., Yeh, Y., Galarraga, J. H., Uman, S., & Burdick, J. A. (2018) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.36323 · Cited by 288 · see citing works
Self-citationKhetan, S., & Burdick, J. A. (2010) · Biomaterials · doi:10.1016/j.biomaterials.2010.07.035 · Cited by 281 · see citing works
Self-citationWang, H., Zhu, D., Paul, A., Cai, L., Enejder, A. M. K., Yang, F., & Heilshorn, S. C. (2017) · Advanced Functional Materials · doi:10.1002/adfm.201605609 · Cited by 280 · see citing works
Polysaccharides and Their Derivatives for Versatile Tissue Engineering Application
Khan, F., & Ahmad, S. R. (2013) · Macromolecular Bioscience · doi:10.1002/mabi.201200409 · Cited by 274 · see citing works
O’Connell, C. D., Bella, C. D., Thompson, F., Augustine, C., Beirne, S., Cornock, R., Richards, C. J., Chung, J. H.-Y., Gambhir, S., Yue, Z., et al. (2016) · Biofabrication · doi:10.1088/1758-5090/8/1/015019 · Cited by 274 · see citing works
Matrix degradability controls multicellularity of 3D cell migration
Trappmann, B., Baker, B. M., Polacheck, W. J., Choi, C. K., Burdick, J. A., & Chen, C. S. (2017) · Nature Communications · doi:10.1038/s41467-017-00418-6 · Cited by 266 · see citing works
Self-citationKim, I. L., Khetan, S., Baker, B. M., Chen, C. S., & Burdick, J. A. (2013) · Biomaterials · doi:10.1016/j.biomaterials.2013.04.004 · Cited by 249 · see citing works
Self-citationAdvancing Frontiers in Bone Bioprinting
Ashammakhi, N. A., Hasan, A., Kaarela, O. I., Byambaa, B., Sheikhi, A., Gaharwar, A. K., & Khademhosseini, A. (2019) · Advanced Healthcare Materials · doi:10.1002/adhm.201801048 · Cited by 245 · see citing works
Schuurmans, C. C. L., Mihajlovic, M., Hiemstra, C., Ito, K., Hennink, W. E., & Vermonden, T. (2020) · Biomaterials · doi:10.1016/j.biomaterials.2020.120602 · Cited by 243 · see citing works
Engineering bioinks for 3D bioprinting
Decante, G., Costa, J. B., Silva‐Correia, J., Collins, M. N., Reis, R. L., & Oliveira, J. M. (2021) · Biofabrication · doi:10.1088/1758-5090/abec2c · Cited by 240 · see citing works
Aravamudhan, A., Ramos, D. M., Nada, A. A., & Kumbar, S. G. (2014) · Natural and Synthetic Biomedical Polymers · doi:10.1016/b978-0-12-396983-5.00004-1 · Cited by 238 · see citing works
Chen, J., Yang, J., Wang, L., Zhang, X., Heng, B. C., Wang, D., & Ge, Z. (2020) · Bioactive Materials · doi:10.1016/j.bioactmat.2020.11.020 · Cited by 238 · see citing works
Micromolding of photocrosslinkable hyaluronic acid for cell encapsulation and entrapment
Khademhosseini, A., Eng, G. M., Yeh, J., Fukuda, J., Blumling, J., Langer, R., & Burdick, J. A. (2006) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.30821 · Cited by 236 · see citing works
Self-citationCustom Design of the Cardiac Microenvironment With Biomaterials
Davis, M. E., Hsieh, P. C. H., Grodzinsky, A. J., & Lee, R. T. (2005) · Circulation Research · doi:10.1161/01.res.0000173376.39447.01 · Cited by 234 · see citing works
3D Culture of Chondrocytes in Gelatin Hydrogels with Different Stiffness
Li, X., Chen, S., Li, J., Wang, X., Zhang, J., Kawazoe, N., & Chen, G. (2016) · Polymers · doi:10.3390/polym8080269 · Cited by 234 · see citing works
Chung, C., Beecham, M., Mauck, R. L., & Burdick, J. A. (2009) · Biomaterials · doi:10.1016/j.biomaterials.2009.04.040 · Cited by 229 · see citing works
Self-citationKuo, C. K., & Ma, P. X. (2007) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.31375 · Cited by 228 · see citing works
Design and preparation of polymeric scaffolds for tissue engineering
Weigel, T., Schinkel, G., & Lendlein, A. (2006) · Expert Review of Medical Devices · doi:10.1586/17434440.3.6.835 · Cited by 228 · see citing works
Degradable and Removable Tough Adhesive Hydrogels
Freedman, B. R., Uzun, O., Luna, N. M. M., Rock, A., Clifford, C., Stoler, E., Östlund-Sholars, G., Johnson, C., & Mooney, D. J. (2021) · Advanced Materials · doi:10.1002/adma.202008553 · Cited by 227 · see citing works
Caliari, S. R., Perepelyuk, M., Cosgrove, B. D., Tsai, S. J., Lee, G. Y., Mauck, R. L., Wells, R. G., & Burdick, J. A. (2016) · Scientific Reports · doi:10.1038/srep21387 · Cited by 218 · see citing works
Self-citationErickson, I. E., Huang, A. H., Chung, C., Li, R. T., Burdick, J. A., & Mauck, R. L. (2009) · Tissue Engineering Part A · doi:10.1089/ten.tea.2008.0099 · Cited by 218 · see citing works
Self-citationChoh, S.-Y., Cross, D. P., & Wang, C. (2011) · Biomacromolecules · doi:10.1021/bm101451k · Cited by 220 · see citing works
- Authors
- Burdick, J. A., Chung, C., Jia, X., Randolph, M. A., & Langer, R.
- Affiliations
- Harvard University (US)
- Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 and Division of Plastic Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02115
- Venue
- Biomacromolecules
- Publisher
- American Chemical Society (ACS)
- Published
- 2004-11-24
- Volume
- 6
- Issue
- 1
- Pages
- 386-391
- ISSN
- 1525-7797 (ISSN-L)
- 1526-4602
- PMC
- PMC2678566
- OpenAlex
- W2022836746
- Cited by
- 777
- References
- 29
- Citations by year
- 2026: 24 · 2025: 30 · 2024: 33 · 2023: 26 · 2022: 35 · 2021: 41 · 2020: 64 · 2019: 41 · 2018: 46 · 2017: 55 · 2016: 44 · 2015: 44 · 2014: 33 · 2013: 48 · 2012: 36
- FWCI
- 6.89
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Biomacromolecules (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institute of Dental and Craniofacial Research (5R01 DE-13023-05, K22DE015761, K22 DE-015761-01, R01DE013023)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Physical Sciences › Materials Science › Biomaterials › Silk-based biomaterials and applications
- Keywords
- hyaluronic acid, photopolymerization, hydrogels, controlled degradation, hyaluronidase, swelling behavior, compressive modulus, 3T3 fibroblasts, auricular chondrocytes, neocartilage formation, tissue engineering scaffolds
- MeSH terms
- Anhydrides, Animals, Carbohydrate Sequence, Cell Survival, Fibroblasts, Hyaluronic Acid, Molecular Sequence Data, Molecular Weight, Photochemistry, Polymers, Time Factors, 3T3 Cells, Hydrogels, Cell Proliferation, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
