Cited by 313Microbial Inactivation MethodsAlgal biology and biofuel productionAtmospheric-Pressure Plasma Applications

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Pulsed electric field assisted extraction of intracellular valuables from microalgae

Goettel, Martina, Eing, Christian, Gusbeth, Christian, Straessner, Ralf, Frey, Wolfgang·2013·Algal Research

DOI 10.1016/j.algal.2013.07.004

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Ullmann's Encyclopedia of Industrial Chemistry
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“…electric field (PEF), though it has lesser yield as compared to mechanical disruption such as High-Pressure Homogenizer (HPH) or bead mill, for cell disruption can be performed with prior pre-Table treatment of algal cells to break down the outer strong cell wall and membrane (Goettel et al. 2013).”
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“For extracting intracellular components from microalgae [83], red beetroot pigment [84], anthocyanins from purple-fleshed potato [85], phenolic compounds from Merlot grapes [86], polyphenols from grape skin [87], and many more, PEF has shown beneficial results.”
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“Meanwhile, irreversible electroporation concept is widely applied in food industries for microbial inactivation [64, 65], in engineering for lipid extraction [12, 13], in biotechnology for pigments, protein and carbohydrates extraction [66-68] and in wastewater treatment to kill pathogens and bacterial [12, 18, 69, 70…”
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“[16] on the extraction of intracellular valuable from A.”
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“Recently, Goettel et al. (2013) found that prior to lipid extraction, pulse electric field (PEF) treatment for cell wall disruption can enhance the effectiveness of extraction.”
Background
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“[78] evaluated the application of the pulsed electric field procedure for the disruption of Auxenochlorella protothecoides cells.”
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Background
Chemical Pretreatment of Algal Biomass
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“Immediately after nsPEF deliveries, the suspensions were incubated for 1 h at room temperature ( Zgalin et al. 2012; Goettel et al. 2013).”
Method
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“Nevertheless, it was shown that the PEF treatment of microalgae is independent of the biomass concentration up to concentrations 160 g l−1 for Auxenochlorella protothecoides (Goettel et al., 2013) and 40 g l−1 for Chlorella vulgaris (Rego et al.”
Background
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“In different PEF treatments, sonication permeabilizes the cell membrane and the cell wall using ultrasound waves based on bubble cavitation [46,64–66].”
Method
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“In [13], it is shown, for suspensions with a biomass content of 100-g dry weight (DW) per kilogram suspension, the electrical energy input necessary for considerable cell rupture was in the range of 1-MJ/kg dried algae, which is about four to seven times less energy than needed in the high pressure homogenizer commonl…”
BackgroundMethod
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“This energy input was fitting for this study since it allowed for the immediate total permeabilization of the cells and high gain of intracellular product (according to the previous studies) while preventing any thermal effect on the microalgae due to overheating [34].”
BackgroundSelf-citation
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“At a high transmembrane voltage, the alignment of the phospholipids changes, resulting in a loss of the protection of the membrane, which becomes permeable, a phenomenon known as “electroporation or electropermeabilization” [37].”
Background
Pulsed Electric Field–Assisted Cell Permeabilization of Microalgae (Haematococcus pluvialis) for Milking of Value-Added Compounds
Ahirwar, A., Khan, M. J., Sirotiya, V., Mourya, M., Rai, A., Schoefs, B., Marchand, J., Varjani, S., & Vinayak, V. (2022) · BioEnergy Research · doi:10.1007/s12155-022-10414-4 · Cited by 22
“Auxenochlorella protothecoides SAG 211-7a Soluble value-added compounds 32 kV·cm−1 30 [118]”
Background
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Spent Brewer’s Yeast Lysis Enables a Best Out of Waste Approach in the Beer Industry
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“As the transmembrane potential is formed, phospholipidic rearrangements occur leading to pore formation, which is known as electroporation or electropermeabilization [66,67].”
Downstream Processes in a Microalgae Biorefinery: Cascaded Enzymatic Hydrolysis and Pulsed Electric Field as Green Solution
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“…of microalgal and cyanobacterial cell membranes, such as those of C. vulgaris , C. reinhardtii , Auxenochlorella protothecoides , and A. platensis , thereby enhancing the extractability of valuable compounds, including carbohydrates, water-soluble proteins, and phenolic compounds [9,51,53,61,70].”
Influential
Nanosecond pulsed electric fields trigger cell differentiation in Chlamydomonas reinhardtii
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“include the extraction of cellular ingredients [4], or even the prolongation of the shelf life for food [5].”
BackgroundMethodSelf-citation
Extracting Water-Soluble Proteins from the Red Macroalgae Gracilaria sp. with Pulsed Electric Field in a Continuous Process
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Background
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“For large volumes the PEF treatment was conducted in continuous ﬂow in a uniform-ﬁeld ﬂow chamber with a volume of 525 l l, enclosed by two planar stainless steel electrodes with a gap of 2 mm in-between [35].”
Self-citation
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BackgroundResultInfluentialSelf-citation
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Self-citation

Authors: Goettel, M., Eing, C., Gusbeth, C., Straessner, R., & Frey, W.
Affiliations: Karlsruhe Institute of Technology (DE)
Corresponding author: Goettel, Martina
ORCID: Gusbeth, Christian
Frey, Wolfgang
Venue: Algal Research
Publisher: Elsevier BV
Published: 2013-10
Volume: 2
Issue: 4
Pages: 401-408
ISSN: 2211-9264 (ISSN-L)
DOI: 10.1016/j.algal.2013.07.004
OpenAlex: W2063784194
Cited by: 313
References: 33
Citations by year: 2026: 16 · 2025: 21 · 2024: 28 · 2023: 24 · 2022: 30 · 2021: 32 · 2020: 40 · 2019: 25 · 2018: 22 · 2017: 25 · 2016: 16 · 2015: 20 · 2014: 14
FWCI: 14.64
Type: journal article
Language: en
Open access: closed
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations: Algal Research (publishedVersion)
Funders: German Helmholtz Association
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Microbial Inactivation Methods
Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
Physical Sciences › Physics and Astronomy › Atomic and Molecular Physics, and Optics › Atmospheric-Pressure Plasma Applications
Keywords: pulsed electric field, Auxenochlorella protothecoides, microalgae, cell disintegration, electric field strength, biomass concentration, biorefinery

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