Comparison of several methods for effective lipid extraction from microalgae
Abstract
Various methods, including autoclaving, bead-beating, microwaves, sonication, and a 10% NaCl solution, were tested to identify the most effective cell disruption method. The total lipids from Botryococcus sp., Chlorella vulgaris, and Scenedesmus sp. were extracted using a mixture of chloroform and methanol (1:1). The lipid contents from the three species were 5.4-11.9, 7.9-8.1, 10.0-28.6, 6.1-8.8, and 6.8-10.9 g L(-1) when using autoclaving, bead-beating, microwaves, sonication, and a 10% NaCl solution, respectively. Botryococcus sp. showed the highest oleic acid productivity at 5.7 mg L(-1)d(-1) when the cells were disrupted using the microwave oven method. Thus, among the tested methods, the microwave oven method was identified as the most simple, easy, and effective for lipid extraction from microalgae.
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Compared to photobioreactors, open ponds have disadvantages such as low biomass yield, high harvesting costs, and the possibility of attack weeds, wild algae, microorganisms, and diseases, which all further reduce algae yield.
“Compared to photobioreactors, open ponds have disadvantages such as low biomass yield, high harvesting costs, and the possibility of attack weeds, wild algae, microorganisms, and diseases, which all further reduce algae yield [29].”
From The Perspective of Large-Scale Production of Algae Biodiesel (2020) · cites this paper as [29]
In LCA of biodiesel production, there are relatively large discrepancies in estimates of CO2 eq/MJ emissions, with many results exceeding fossil diesel emissions.
“In the life cycle analysis (LCA) of biodiesel production, there are relatively large discrepancies in estimates of CO2 eq/MJ emissions of biofuel produced, with many results exceeding fossil diesel emissions [29].”
From The Perspective of Large-Scale Production of Algae Biodiesel (2020) · cites this paper as [29]
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- Authors
- Lee, J.-Y., Yoo, C., Jun, S.-Y., Ahn, C.-Y., & Oh, H.-M.
- Affiliations
- Korea Research Institute of Bioscience and Biotechnology (KR)
- Corresponding author
- Oh, Hee-Mock
- Venue
- Bioresource Technology
- Publisher
- Elsevier BV
- Published
- 2010-01
- Volume
- 101
- Issue
- 1
- Pages
- S75-S77
- ISSN
- 0960-8524 (ISSN-L)
- OpenAlex
- W2168003071
- Semantic Scholar
- 1daab156a7df4b3f1bde42787ffba7a350ce8564
- Cited by
- 1,279
- References
- 17
- Citations by year
- 2026: 29 · 2025: 28 · 2024: 40 · 2023: 56 · 2022: 63 · 2021: 80 · 2020: 88 · 2019: 116 · 2018: 102 · 2017: 102 · 2016: 88 · 2015: 105 · 2014: 126 · 2013: 100 · 2012: 73
- FWCI
- 24.03
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Bioresource Technology (publishedVersion)
- PubMed (publishedVersion)
- KRIBB Repository (submittedVersion)
- Funders
- Ministry of Education, Science and Technology
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Keywords
- microalgal lipid extraction, sonication, bead beating, autoclaving, Botryococcus, Chlorella vulgaris, Scenedesmus, microalgal lipids
- MeSH terms
- Methanol, Chlorophyta, Cells, Cultured, Chloroform, Chemical Fractionation, Lipids, Solvents, Time Factors, Biomass, Bioreactors
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