The DOI matches its Crossref record; 26 of the 26 references it cites were found in a registry or an index.
Detection of Clostridium perfringens toxin genes in the gut microbiota of autistic children
Abstract
We studied stool specimens from 33 autistic children aged 2-9 years with gastrointestinal (GI) abnormalities and 13 control children without autism and without GI symptoms. We performed quantitative comparison of all Clostridium species and Clostridium perfringens strains from the fecal microbiota by conventional, selective anaerobic culture methods. We isolated C. perfringens strains and performed PCR analysis for the main C. perfringens toxin genes, alpha, beta, beta2, epsilon, iota and C. perfringens enterotoxin gene. Our results indicate that autistic subjects with gastrointestinal disease harbor statistically significantly (p = 0.031) higher counts of C. perfringens in their gut compared to control children. Autistic subjects also harbor statistically significantly (p = 0.015) higher counts of beta2-toxin gene-producing C. perfringens in their gut compared to control children, and the incidence of beta2-toxin gene-producing C. perfringens is significantly higher in autistic subjects compared to control children (p = 0.014). Alpha toxin gene was detected in all C. perfringens strains studied. C. perfringens enterotoxin gene was detected from three autistic and one control subject. Beta, epsilon, and iota toxin genes were not detected from autistic or control subjects.
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In the citing paper
“Clostridium perfringens at a species level had evidence for increased absolute and relative abundance in children with ASD versus typically developing children [34, 38, 44].”
From Gut microbiota changes in children with autism spectrum disorder: a systematic review (2020) · cites this paper as [34]
In the citing paper
“There are a number of studies, however, that demonstrate a difference in Clostridia levels in children with ASD that, interestingly, is a target for vancomycin 79–82.”
From Gastrointestinal Issues and Autism Spectrum Disorder (2020) · cites this paper as [81]
Citations
Cites
1 retracted
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The RDP (Ribosomal Database Project)
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Wadsworth anaerobic bacteriology manual
Sutter, V. L., Citron, D. M., & Finegold, S. M. (1980) · Medical Entomology and Zoology · Cited by 1,388
Self-citationThe RDP-II (Ribosomal Database Project)
Maidak, B. L. (2001) · Nucleic Acids Research · doi:10.1093/nar/29.1.173 · Cited by 1,180
Parracho, H. M., Bingham, M. O., Gibson, G. R., & McCartney, A. L. (2005) · Journal of Medical Microbiology · doi:10.1099/jmm.0.46101-0 · Cited by 784
Gastrointestinal Microflora Studies in Late‐Onset Autism
Finegold, S. M., Molitoris, D., Song, Y., Liu, C., Vaisanen, M., Bolte, E., McTeague, M., Sandler, R., Wexler, H., Marlowe, E. M., et al. (2002) · Clinical Infectious Diseases · doi:10.1086/341914 · Cited by 742
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James, S. J., Melnyk, S., Jernigan, S., Cleves, M. A., Halsted, C. H., Wong, D. H., Cutler, P., Bock, K., Boris, M., Bradstreet, J., et al. (2006) · American Journal of Medical Genetics Part B Neuropsychiatric Genetics · doi:10.1002/ajmg.b.30366 · Cited by 618
Short-Term Benefit From Oral Vancomycin Treatment of Regressive-Onset Autism
Sandler, R. H., Finegold, S. M., Bolte, E. R., Buchanan, C. P., Maxwell, A. P., Väisänen, M.-L., Nelson, M. N., & Wexler, H. M. (2000) · Journal of Child Neurology · doi:10.1177/088307380001500701 · Cited by 614
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Research works printed but not linked: 1
- Carman, 2008, Clostridium perfringens toxin genotypes in the feces of healthy North Americans, Anaerobe, 2008, 102, 10.1016/j.anaerobe.2008.01.003 · DOI
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Gut microbiota and mental issues in humans
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Metadata
- Authors
- Finegold, S. M., Summanen, P. H., Downes, J., Corbett, K., & Komoriya, T.
- Affiliations
- University of California, Los Angeles (US)
- VA West Los Angeles Medical Center (US)
- Nihon University (JP)
- Corresponding author
- Finegold, Sydney M.
- Venue
- Anaerobe
- Publisher
- Elsevier BV
- Published
- 2017-06
- Volume
- 45
- Pages
- 133-137
- ISSN
- 1075-9964 (ISSN-L)
- OpenAlex
- W2587897382
- Cited by
- 110
- References
- 26
- Citations by year
- 2026: 3 · 2025: 9 · 2024: 12 · 2023: 11 · 2022: 16 · 2021: 16 · 2020: 13 · 2019: 15 · 2018: 12 · 2017: 3
- FWCI
- 9.10
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Anaerobe (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Autism Research Institute
- Topics
- Health Sciences › Medicine › Infectious Diseases › Clostridium difficile and Clostridium perfringens research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
- Keywords
- Clostridium perfringens, autism spectrum disorder, gut microbiota, toxin genes, beta2 toxin, alpha-toxin, enterotoxin, gastrointestinal anomalies, fecal microbiota, children
- MeSH terms
- Autistic Disorder, Bacterial Toxins, Bacteriological Techniques, Child, Child, Preschool, Clostridium perfringens, Feces, Female, Humans, Male, Polymerase Chain Reaction, Gastrointestinal Tract
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