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Cited by 73Cytomegalovirus and herpesvirus researchGarlic and Onion StudiesHerpesvirus Infections and Treatments

Experimental study on the action of allitridin against human cytomegalovirus in vitro: Inhibitory effects on immediate-early genes

Zhen, Hong, Fang, Feng, Ye, Du-yun, Shu, Sai-nan, Zhou, Yu-feng, Dong, Yong-sui, Nie, Xing-cao, Li, Ge·2006·Antiviral Research
DOI 10.1016/j.antiviral.2006.03.017
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Abstract

Garlic (Allium sativum) extraction has been reported having anti-HCMV efficacy. This study was aimed to investigate the effect of allitridin (diallyl trisulfide, a compound from A. sativum extraction) on the replication of HCMV and the expression of viral immediate-early genes. In HCMV plaque-reduction assay, allitridin appeared a dose-dependent inhibitory ability with EC(50) value of 4.2 microg/ml (selective index, SI=16.7). Time-of-addition and time-of-removal studies showed that allitridin inhibited HCMV replication in earlier period of viral cycle before viral DNA synthesis. Both immediate early gene (ie1) transcription and IEA (IE(1)72 and IE(2)86) expression was suppressed by allitridin, but not by GCV in a single HCMV cycle format. In addition, allitridin appeared stronger inhibition on IE(2)86 than on IE(1)72. Decrease of viral DNA load in infected cells was also detected under allitridin treatment, probably due to an indirect consequence of the reduction in ie gene transcription. In summary, this study indicated that allitridin has anti-HCMV activity and the mechanism is associated with suppression of ie gene transcription.

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  • Aqueous, ethanolic, and dichloromethane A. sativum extracts exhibited anthelmintic activity against Haemonchus contortus, with the ethanolic extract being the most effective, while aqueous garlic extract showed potent activity against Trichuris muris and Angiostrongylus cantonensis.

    “For instance, an in vitro study revealed that the aqueous, ethanolic, and dichloromethane A. sativum extracts exhibited anthelmintic activity against Haemonchus contortus and the ethanolic extract was the most effective one, while aqueous garlic extract showed potent activity against Trichuris muris and Angiostrongylus cantonensis [58].”

    From Chemical Constituents and Pharmacological Activities of Garlic (Allium sativum L.): A Review (2020) · cites this paper as [58]

  • DATS was effective against HCMV replication and viral immediate-early gene expression and acts by enhancing natural killer-cell (NK-cell) activity that destroys virus-infected cells.

    “Moreover, DATS was effective against the HCMV replication and viral immediate-early gene expression and it acts by enhancing natural killer-cell (NK-cell) activity that destroys virus-infected cells [58].”

    From Chemical Constituents and Pharmacological Activities of Garlic (Allium sativum L.): A Review (2020) · cites this paper as [58]

  • Allicin and DATS showed antiparasitic activity against Entamoeba histolytica, Plasmodium falciparum, Babesia, Theleria, Trypanosoma brucei, and Giardia lamblia.

    “Moreover, allicin and DATS, phytochemicals isolated from garlic extract, showed antiparasitic activity against Entamoeba histolytica, Plasmodium falciparum, Babesia, Theleria, Trypanosoma brucei, and Giardia lamblia [58].”

    From Chemical Constituents and Pharmacological Activities of Garlic (Allium sativum L.): A Review (2020) · cites this paper as [58]

  • Saponins extracted from A. sativum exhibited antifungal activity against Botrytis cinerea and Trichoderma harzianum.

    “In addition to that, saponins extracted from A. sativum exhibited antifungal activity against Botrytis cinerea and Trichoderma harzianum [58].”

    From Chemical Constituents and Pharmacological Activities of Garlic (Allium sativum L.): A Review (2020) · cites this paper as [58]

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Authors
Zhen, H., Fang, F., Ye, D.-Y., Shu, S.-N., Zhou, Y.-F., Dong, Y.-S., Nie, X.-C., & Li, G.
Affiliations
Tongji Hospital (CN)
ORCID
Li, Ge
Venue
Antiviral Research
Publisher
Elsevier BV
Published
2006-10
Volume
72
Issue
1
Pages
68-74
ISSN
0166-3542 (ISSN-L)
DOI
10.1016/j.antiviral.2006.03.017
OpenAlex
W2042947858
Cited by
73
References
30
Citations by year
2026: 3 · 2025: 4 · 2024: 3 · 2023: 10 · 2022: 10 · 2021: 10 · 2020: 9 · 2019: 2 · 2018: 1 · 2017: 3 · 2016: 1 · 2014: 1 · 2013: 4 · 2012: 1
FWCI
1.46
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Antiviral Research (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Natural Science Foundation of China
Topics
  • Health Sciences › Medicine › Epidemiology › Cytomegalovirus and herpesvirus research
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Garlic and Onion Studies
  • Health Sciences › Medicine › Epidemiology › Herpesvirus Infections and Treatments
Keywords
diallyl trisulfide, garlic, human cytomegalovirus, immediate early genes, viral replication inhibition, viral DNA load
MeSH terms
Allyl Compounds, Antiviral Agents, Cell Line, Cytomegalovirus, Dose-Response Relationship, Drug, Genes, Viral, Humans, Sulfides, Transcription, Genetic, Viral Proteins, Virus Replication, Trans-Activators, Gene Expression, Genes, Immediate-Early, Immediate-Early Proteins

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