Isolation and characterization of potential probiotic Lactobacilli from leaves of food plants for possible additives in pellet feeding
Open accessAbstract
Bacteria strains isolated from plant leaves of papaya, cassava, sugarcane, yam, and taro, were investigated for their potential probiotic properties. Four isolates from food crop plant leaves were identified as Lactobacillus plantarum, Enterococcus faecalis, Lactobacillus paraplantarum, and Weissella paramesenteroides by sequence of 16S rDNA. The five isolates exhibited tolerance to simulated intestinal juices, and these were selected to evaluate their probiotic properties by biochemical characterization and in vivo testing as a pelleted feed additive. All 5 strains were susceptible to Streptomycin, Penicillin, Chloramphenicol, Ampicillin, and Tetracycline, and exhibited antimicrobial activity against Gram-positive Bacilus cereus, Listeria monocytogenes, Staphylococcus aureus, and the Gram-negative Escherichia coli pathogen. Furthermore, the lactobacilli isolates W. paramesenteroides C02, E. faecalis S04, L. paraplantarum P01, L. plantarum T03, and W. paramesenteroides Y05 exhibited rapid growth in skimmed milk with no nutritional supplement to 108 cfu·mL−1 after 24 h incubation. In vivo analysis of swine feeds after granulation exhibited 109–1010 cfu·kg−1 of L. plantarum, E faecalis, and W. paramesenteroides, which confirmed the probiotic potential and application of the lactobacilli strains isolated from plant leaves in granulated or pelleted feed as additives.
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Several LAB species have been characterized and used as probiotics that successfully promote human health.
“Some lactobacilli are considered probiotics due to their beneficial effects on host health [72,78].”
From Lactic Acid Bacteria in Raw-Milk Cheeses: From Starter Cultures to Probiotic Functions (2022) · cites this paper as [72]
Some lactobacilli are considered probiotics due to their beneficial effects on host health.
“Some lactobacilli are considered probiotics due to their beneficial effects on host health [72,78].”
From Lactic Acid Bacteria in Raw-Milk Cheeses: From Starter Cultures to Probiotic Functions (2022) · cites this paper as [72]
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Lactic Acid Bacteria in Raw-Milk Cheeses: From Starter Cultures to Probiotic Functions
Coelho, M. C., Malcata, F. X., & Silva, C. C. G. (2022) · Foods · doi:10.3390/foods11152276 · Cited by 276 · see citing works
“Some lactobacilli are considered probiotics due to their beneficial effects on host health [72,78].”
BackgroundProbiotics in the dairy industry—Advances and opportunities
Gao, J., Li, X., Zhang, G., Sadiq, F. A., Simal‐Gandara, J., Xiao, J., & Sang, Y. (2021) · Comprehensive Reviews in Food Science and Food Safety · doi:10.1111/1541-4337.12755 · Cited by 195 · see citing works
- Authors
- Samedi, L., & Charles, A. L.
- Affiliations
- National Pingtung University of Science and Technology (TW)
- Corresponding author
- Charles, Albert Linton
- Venue
- Annals of Agricultural Sciences
- Publisher
- Elsevier BV
- Published
- 2019-06
- Volume
- 64
- Issue
- 1
- Pages
- 55-62
- ISSN
- 0570-1783 (ISSN-L)
- OpenAlex
- W2946669491
- Cited by
- 59
- References
- 52
- Citations by year
- 2026: 5 · 2025: 9 · 2024: 10 · 2023: 14 · 2022: 9 · 2021: 10 · 2020: 1 · 2019: 1
- FWCI
- 4.05
- Type
- journal article
- Language
- en
- Update notices
- erratum (2021-06-01)
- Open access
- gold
- doi.org
- Licence
- Locations
- Annals of Agricultural Science Cairo (publishedVersion, cc-by-nc-nd)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Directorate-General for International Cooperation and Development
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
- Life Sciences › Immunology and Microbiology › Immunology › Aquaculture disease management and microbiota
- Keywords
- probiotics, Lactobacillus, leaves, papaya, cassava, sugarcane, yam, taro, Lactobacillus plantarum, Enterococcus faecalis, Lactobacillus paraplantarum, Weissella paramesenteroides, pig feed, antimicrobial activity, acid resistance, 16S rRNA identification, in vivo survival
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