Cited by 23Neurogenesis and neuroplasticity mechanismsNeuroscience and Neuropharmacology ResearchCannabis and Cannabinoid Research

Pyridoxine improves hippocampal cognitive function via increases of serotonin turnover and tyrosine hydroxylase, and its association with CB1 cannabinoid receptor-interacting protein and the CB1 cannabinoid receptor pathway

Jung, Hyo Young, Kim, Dae Won, Nam, Sung Min, Kim, Jong Whi, Chung, Jin Young, Won, Moo-Ho, Seong, Je Kyung, Yoon, Yeo Sung +2 more·2017·Biochimica et Biophysica Acta (BBA) - General Subjects

Abstract

Background In the present study, we investigated the effects of pyridoxine on hippocampal functions and changes in protein profiles based on the proteomic approach.Methods Eight-week-old mice received intraperitoneal injections of physiological saline (vehicle) or 350mg/kg pyridoxine twice a day for 21days.Results Phosphoglycerate mutase 1 was up-regulated, while CB1 cannabinoid receptor-interacting protein 1 (CRIP1) was down-regulated, in the pyridoxine-treated group. Additionally, the serotonin and tyrosine hydroxylase was increased in the hippocampus of the pyridoxine-treated group than in that of the vehicle-treated group. Furthermore, discrimination indices based on the novel object recognition test were significantly higher in the pyridoxine-treated group than in the vehicle-treated group. Administration of CRIP1a siRNA significantly increases the discrimination index as well as cell proliferation and neuroblast differentiation in the dentate gyrus. In addition, the administration of rimonabant, a CB1 cannabinoid receptor antagonist, for 3weeks significantly decreased the novel object recognition memory, the tyrosine hydroxylase level, the amount of cell proliferation, and neuroblast differentiation in the dentate gyrus. Treatment with pyridoxine significantly increased novel object recognition memory, but slightly ameliorated rimonabant-induced reduction in serotonin, the tyrosine hydroxylase level, the amount of cell proliferation, and neuroblast differentiation in the dentate gyrus.Conclusion These results suggest that pyridoxine promotes hippocampal functions by increasing serotonin and tyrosine hydroylase immunoreactivity in the hippocampus. This positive effect may be associated with CRIP1a and CB1 cannabinoid receptor function.General significance Vitamin-B 6 enhances hippocampal functions and this is closely associated with CRIP1a and CB1 cannabinoid receptors.

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Crossref · 10.1016/j.bbagen.2017.09.006

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Authors
Jung, H. Y., Kim, D. W., Nam, S. M., Kim, J. W., Chung, J. Y., Won, M.-H., Seong, J. K., Yoon, Y. S., Yoo, D. Y., & Hwang, I. K.
Affiliations
  • Seoul National University (KR)
  • Gangneung–Wonju National University (KR)
  • Kangwon National University (KR)
Corresponding author
  • Yoo, Dae Young
  • Hwang, In Koo
Venue
Biochimica et Biophysica Acta (BBA) - General Subjects
Publisher
Elsevier BV
Published
2017-12
Volume
1861
Issue
12
Pages
3142-3153
ISSN
0304-4165 (ISSN-L)
OpenAlex
W2754495124
Cited by
23
References
68
Citations by year
2026: 1 · 2025: 1 · 2024: 2 · 2023: 1 · 2022: 2 · 2021: 1 · 2020: 8 · 2019: 3 · 2018: 4
FWCI
1.73
Type
journal article
Language
en
Open access
closed
Locations
  • Biochimica et Biophysica Acta (BBA) - General Subjects (publishedVersion)
  • PubMed (publishedVersion)
  • Seoul National University Open Repository (Seoul National University) (submittedVersion)
Funders
  • Seoul National University
  • National Research Foundation of Korea (NRF-2013R1A1A2059364, NRF-2016R1A2B4009156, NRF-2015M3A9D5A01076747)
Topics
  • Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Health Sciences › Medicine › Pharmacology › Cannabis and Cannabinoid Research
Keywords
pyridoxine, hippocampus, cognitive function, serotonin turnover, tyrosine hydroxylase, CB1 receptor, CRIP1, rimonabant, novel object recognition memory, dentate gyrus, neuroblast differentiation, cell proliferation, phosphoglycerate mutase 1, siRNA knockdown
MeSH terms
Animals, Carrier Proteins, Cognition, Hippocampus, Immunohistochemistry, Male, Memory, Mice, Inbred C57BL, Pyridoxine, Serotonin, Tyrosine 3-Monooxygenase, Receptor, Cannabinoid, CB1, Mice, LIM Domain Proteins

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