Localization of Ras signaling complex in budding yeast
Open accessAbstract
In Saccharomyces cerevisiae, cAMP/pKA pathway plays a major role in metabolism, stress resistance and proliferation control. cAMP is produced by adenylate cyclase, which is activated both by Gpr1/Gpa2 system and Ras proteins, regulated by Cdc25/Sdc25 guanine exchange factors and Ira GTPase activator proteins. Recently, both Ras2 and Cdc25 RasGEF were reported to localize not only in plasma membrane but also in internal membranes. Here, the subcellular localization of Ras signaling complex proteins was investigated both by fluorescent tagging and by biochemical cell membrane fractionation on sucrose gradients. Although a consistent minor fraction of Ras signaling complex components was found in plasma membrane during exponential growth on glucose, Cdc25 appears to localize mainly on ER membranes, while Ira2 and Cyr1 are also significantly present on mitochondria. Moreover, PKA Tpk1 catalytic subunit overexpression induces Ira2 protein to move from mitochondria to ER membranes. These data confirm the hypothesis that different branches of Ras signaling pathways could involve different subcellular compartments, and that relocalization of Ras signaling complex components is subject to PKA control.
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In S. cerevisiae, Ira2 interacts with the protein kinase A regulatory subunit, and Cyr1 and Ira2 have both been found at the plasma membrane and on mitochondria.
“In S. cerevisiae, Ira2 has been shown to interact with the protein kinase A regulatory subunit, and Cyr1 and Ira2 have both been found at the plasma membrane and on mitochondria [78]”
From Mitochondrial Activity and Cyr1 Are Key Regulators of Ras1 Activation of C. albicans Virulence Pathways (2015) · cites this paper as [78]
The Results and Discussion report that Cyr1 and Ira2 occur on mitochondria and that a minor fraction of both is associated with plasma-membrane fractions. They do not report an interaction between Ira2 and the PKA regulatory subunit; the PKA experiments instead concern overexpression of the catalytic subunit Tpk1. (LLM, conf=0.88)
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“cerevisiae, Ira2 has been shown to interact with the protein kinase A regulatory subunit, and Cyr1 and Ira2 have both been found at the plasma membrane and on mitochondria [78] providing further support for the potential for interactions between Cyr1 and Ira2, probably in ways that respond to mitochondrial activity [7…”
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- Authors
- Belotti, F., Tisi, R., Paiardi, C., Rigamonti, M., Groppi, S., & Martegani, E.
- Affiliations
- University of Milano-Bicocca (IT)
- Corresponding author
- Martegani, Enzo
- Venue
- Biochimica et Biophysica Acta (BBA) - Molecular Cell Research
- Publisher
- Elsevier BV
- Published
- 2012-07
- Volume
- 1823
- Issue
- 7
- Pages
- 1208-1216
- ISSN
- 0167-4889 (ISSN-L)
- OpenAlex
- W1985464808
- Cited by
- 35
- References
- 59
- Citations by year
- 2025: 2 · 2024: 2 · 2023: 3 · 2022: 4 · 2021: 3 · 2020: 2 · 2019: 1 · 2018: 4 · 2017: 1 · 2016: 2 · 2015: 2 · 2014: 6 · 2013: 2 · 2012: 1
- FWCI
- 1.41
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Biochimica et Biophysica Acta (BBA) - Molecular Cell Research (publishedVersion)
- PubMed (publishedVersion)
- BOA (University of Milano-Bicocca) (submittedVersion)
- Funders
- UNICELLSYS
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Keywords
- subcellular localization, Saccharomyces cerevisiae, cAMP/PKA signaling, RAS2, Cdc25, IRA2, CYR1, TPK1, plasma membrane, endoplasmic reticulum membrane, mitochondria, guanine nucleotide exchange factor, adenylate cyclase, protein relocalization
- MeSH terms
- Endoplasmic Reticulum, Guanosine Diphosphate, Guanosine Triphosphate, Saccharomyces cerevisiae, Signal Transduction, Cyclic AMP-Dependent Protein Kinases, ras Proteins, Catalytic Domain, Protein Transport, Saccharomyces cerevisiae Proteins, Multiprotein Complexes, Mitochondrial Membranes
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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