New insights into the functions of ACBD4/5-like proteins using a combined phylogenetic and experimental approach across model organisms.
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Abstract
Acyl-CoA binding domain-containing proteins (ACBDs) perform diverse but often uncharacterised functions linked to cellular lipid metabolism. Human ACBD4 and ACBD5 are closely related peroxisomal membrane proteins, involved in tethering of peroxisomes to the ER and capturing fatty acids for peroxisomal β-oxidation. ACBD5 deficiency causes neurological abnormalities including ataxia and white matter disease. Peroxisome-ER contacts depend on an ACBD4/5-FFAT motif, which interacts with ER-resident VAP proteins. As ACBD4/5-like proteins are present in most fungi and all animals, we combined phylogenetic analyses with experimental approaches to improve understanding of their evolution and functions. Notably, all vertebrates exhibit gene sequences for both ACBD4 and ACBD5, while invertebrates and fungi possess only a single ACBD4/5-like protein. Our analyses revealed alterations in domain structure and FFAT sequences, which help understanding functional diversification of ACBD4/5-like proteins. We show that the Drosophila melanogaster ACBD4/5-like protein possesses a functional FFAT motif to tether peroxisomes to the ER via Dm_Vap33. Depletion of Dm_Acbd4/5 caused peroxisome redistribution in wing neurons and reduced life expectancy. In contrast, the ACBD4/5-like protein of the filamentous fungus Ustilago maydis lacks a FFAT motif and does not interact with Um_Vap33. Loss of Um_Acbd4/5 resulted in an accumulation of peroxisomes and early endosomes at the hyphal tip. Moreover, lipid droplet numbers increased, and mitochondrial membrane potential declined, implying altered lipid homeostasis. Our findings reveal differences between tethering and metabolic functions of ACBD4/5-like proteins across evolution, improving our understanding of ACBD4/5 function in health and disease. The need for a unifying nomenclature for ACBD proteins is discussed.
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Knockout of ACBD5 in HeLa and other mammalian cells did not affect pexophagy.
“knockout of ACBD5 in HeLa and other mammalian cells did not affect pexophagy (Ferdinandusse et al., 2017; Kors et al., 2024).”
From The neurological pathology of peroxisomal ACBD5 deficiency – lessons from patients and mouse models (2025) · cites this paper as Kors et al., 2024
Section 2.6 (p. 9) reports that other studies did not find ACBD5 involved in peroxisome degradation, and Section 3.2 (p. 13) says human ACBD5 does not appear to affect it. The cited paper does not specify ACBD5 knockout in HeLa or other mammalian cells; its own mammalian-cell experiment tested overexpression in H9C2 myoblasts. (LLM, conf=0.84)
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Cites
Krogh, A., Larsson, B., von Heijne, G., & Sonnhammer, E. L. L. (2001) · Journal of Molecular Biology · doi:10.1006/jmbi.2000.4315 · Cited by 12,654 · see citing works
Journal of Biological Chemistry
Kurkinensp, M., CondongT, M. R., Blumberg, B., Barlow, D. P., Quiñones, S., Sausg, J., & Pihlajaniemisyy, T. (2021) · Journal of Biological Chemistry · doi:10.1074/jbc · Cited by 3,409 · see citing works
JPred4: a protein secondary structure prediction server
Drozdetskiy, A., Cole, C., Procter, J. B., & Barton, G. J. (2015) · Nucleic Acids Research · doi:10.1093/nar/gkv332 · Cited by 1,714 · see citing works
New and continuing developments at PROSITE
Sigrist, C. J. A., de Castro, E., Cerutti, L., Cuche, B., Hulo, N., Bridge, A. J., Bougueleret, L., & Xénarios, I. (2012) · Nucleic Acids Research · doi:10.1093/nar/gks1067 · Cited by 1,543 · see citing works
DeepTMHMM predicts alpha and beta transmembrane proteins using deep neural networksPreprint
Hallgren, J., Tsirigos, K. D., Pedersen, M. D., Armenteros, J. J. A., Marcatili, P., Nielsen, H., Krogh, A., & Winther, O. (2022) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2022.04.08.487609 · Cited by 1,528 · see citing works
Pandey, U. B., & Nichols, C. D. (2011) · Pharmacological Reviews · doi:10.1124/pr.110.003293 · Cited by 1,155 · see citing works
IBS: an illustrator for the presentation and visualization of biological sequences
Liu, W., Xie, Y., Ma, J., Luo, X., Nie, P., Zuo, Z., Lahrmann, U., Zhao, Q., Zheng, Y., Zhao, Y. B., et al. (2015) · Bioinformatics · doi:10.1093/bioinformatics/btv362 · Cited by 1,018 · see citing works
Protein Sequence Analysis Using the MPI Bioinformatics Toolkit
Gabler, F., Nam, S., Till, S., Mirdita, M., Steinegger, M., Söding, J., Lupas, A. N., & Alva, V. (2020) · Current Protocols in Bioinformatics · doi:10.1002/cpbi.108 · Cited by 831 · see citing works
Role of long-chain fatty acyl-CoA esters in the regulation of metabolism and in cell signalling
Færgeman, N. J., & Knudsen, J. (1997) · Biochemical Journal · doi:10.1042/bj3230001 · Cited by 681 · see citing works
A Protein Complex Network of Drosophila melanogaster
Guruharsha, K. G., Rual, J.-F. M., Zhai, B., Mintseris, J., Vaidya, P., Vaidya, N., Beekman, C. N., Wong, C. S. F., Rhee, D. Y., Cenaj, O., et al. (2011) · Cell · doi:10.1016/j.cell.2011.08.047 · Cited by 654 · see citing works
A conserved ER targeting motif in three families of lipid binding proteins and in Opi1p binds VAP
Loewen, C. J. R., Roy, A., & Levine, T. P. (2003) · The EMBO Journal · doi:10.1093/emboj/cdg201 · Cited by 607 · see citing works
Gargano, J. W., MARTIN, I., Bhandari, P., & Grotewiel, M. (2005) · Experimental Gerontology · doi:10.1016/j.exger.2005.02.005 · Cited by 494 · see citing works
An HMM model for coiled-coil domains and a comparison with PSSM-based predictions
Delorenzi, M., & Speed, T. (2002) · Bioinformatics · doi:10.1093/bioinformatics/18.4.617 · Cited by 479 · see citing works
Repeated ER–endosome contacts promote endosome translocation and neurite outgrowth
Raiborg, C., Wenzel, E. M., Pedersen, N. M., Olsvik, H. L., Schink, K. O., Schultz, S. W., Vietri, M., Nisi, V., Bucci, C., Brech, A., et al. (2015) · Nature · doi:10.1038/nature14359 · Cited by 415 · see citing works
Banuett, F., & Herskowitz, I. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.15.5878 · Cited by 398 · see citing works
Murphy, S. E., & Levine, T. P. (2016) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/j.bbalip.2016.02.009 · Cited by 325 · see citing works
Identification of genes in the bW/bE regulatory cascade in Ustilago maydis
Brachmann, A., Weinzierl, G., Kämper, J., & Kahmann, R. (2001) · Molecular Microbiology · doi:10.1046/j.1365-2958.2001.02699.x · Cited by 292 · see citing works
VAPs and ACBD5 tether peroxisomes to the ER for peroxisome maintenance and lipid homeostasis.
Hua, R., Cheng, D., Coyaud, É., Freeman, S., Di Pietro, E., Wang, Y., Vissa, A., Yip, C. M., Fairn, G. D., Braverman, N., et al. (2017) · Journal of Cell Biology · doi:10.1083/jcb.201608128 · Cited by 279 · see citing works
Abu-Safieh, L., Alrashed, M., Anazi, S., Alkuraya, H., Khan, A. O., Al-Owain, M., Al-Zahrani, J., Al-Abdi, L., Hashem, M., Al-Tarimi, S., et al. (2013) · Genome Research · doi:10.1101/gr.144105.112 · Cited by 276 · see citing works
Gouy, M., Tannier, É., Comte, N., & Parsons, D. P. (2020) · Methods in molecular biology · doi:10.1007/978-1-0716-1036-7_15 · Cited by 259 · see citing works
ACBD5 and VAPB mediate membrane associations between peroxisomes and the ER
Costello, J. L., Castro, I. G., Hacker, C., Schrader, T. A., Metz, J., Zeuschner, D., Azadi, A. S., Godinho, L. F., Costina, V., Findeisen, P., et al. (2017) · The Journal of Cell Biology · doi:10.1083/jcb.201607055 · Cited by 251 · see citing works
Self-citationHigh levels of Fis1, a pro-fission mitochondrial protein, trigger autophagy
Gomes, L. C., & Scorrano, L. (2008) · Biochimica et Biophysica Acta (BBA) - Bioenergetics · doi:10.1016/j.bbabio.2008.05.442 · Cited by 239 · see citing works
PredictProtein - Predicting Protein Structure and Function for 29 Years
Bernhofer, M., Dallago, C., Karl, T., Satagopam, V., Heinzinger, M., Littmann, M., Olenyi, T., Qiu, J., Schütze, K., Yachdav, G., et al. (2021) · Nucleic Acids Research · doi:10.1093/nar/gkab354 · Cited by 239 · see citing works
Chami, M., Oulès, B., Szabadkai, G., Tacine, R., Rizzuto, R., & Paterlini-Bréchot, P. (2008) · Molecular Cell · doi:10.1016/j.molcel.2008.11.014 · Cited by 235 · see citing works
Holliday, R. (1974) · Springer eBooks · doi:10.1007/978-1-4899-1710-2_31 · Cited by 223 · see citing works
Cryptic peroxisomal targeting via alternative splicing and stop codon read-through in fungi
Freitag, J., Ast, J., & Bölker, M. (2012) · Nature · doi:10.1038/nature11051 · Cited by 184 · see citing works
DeepCoil—a fast and accurate prediction of coiled-coil domains in protein sequences
Ludwiczak, J., Winski, A., Szczepaniak, K., Alva, V., & Dunin-Horkawicz, S. (2018) · Bioinformatics · doi:10.1093/bioinformatics/bty1062 · Cited by 184 · see citing works
Long-chain acyl-CoA esters in metabolism and signaling: Role of acyl-CoA binding proteins
Neess, D., Bek, S., Engelsby, H., Gallego, S. F., & Færgeman, N. J. (2015) · Progress in Lipid Research · doi:10.1016/j.plipres.2015.04.001 · Cited by 181 · see citing works
An ankyrin-repeat and WRKY-domain-containing immune receptor confers stripe rust resistance in wheat
Wang, H., Zou, S., Li, Y., Fan-Yun, L. I. N., & Tang, D. (2020) · Nature Communications · doi:10.1038/s41467-020-15139-6 · Cited by 165 · see citing works
Organelle interplay-peroxisome interactions in health and disease.
Schrader, M., Kamoshita, M., & Islinger, M. (2020) · Journal of Inherited Metabolic Disease · doi:10.1002/jimd.12083 · Cited by 149 · see citing works
Self-citationDembeck, L. M., Böröczky, K., Huang, W., Coby Schal, A., Anholt, R. R. H., & Mackay, T. F. C. (2015) · eLife · doi:10.7554/elife.09861 · Cited by 137 · see citing works
Stiebler, A. C., Freitag, J., Schink, K. O., Stehlik, T., Tillmann, B. A. M., Ast, J., & Bölker, M. (2014) · PLoS Genetics · doi:10.1371/journal.pgen.1004685 · Cited by 133 · see citing works
Peroxisomes, lipid droplets, and endoplasmic reticulum “hitchhike” on motile early endosomes
Guimarães, S., Schuster, M., Bielska, E., Dagdas, G., Kilaru, S., Meadows, B. R. A., Schrader, M., & Steinberg, G. (2015) · The Journal of Cell Biology · doi:10.1083/jcb.201505086 · Cited by 132 · see citing works
Self-citationUstilago maydis, a new fungal model system for cell biology
Steinberg, G., & Pérez‐Martín, J. (2008) · Trends in Cell Biology · doi:10.1016/j.tcb.2007.11.008 · Cited by 131 · see citing works
ACBD5 deficiency causes a defect in peroxisomal very long-chain fatty acid metabolism
Ferdinandusse, S., Falkenberg, K. D., Koster, J., Mooyer, P. A., Jones, R., van Roermund, C. W. T., Pizzino, A., Schrader, M., Wanders, R. J. A., Vanderver, A., et al. (2017) · Journal of Medical Genetics · doi:10.1136/jmedgenet-2016-104132 · Cited by 120 · see citing works
Self-citationMyosin‐5, kinesin‐1 and myosin‐17 cooperate in secretion of fungal chitin synthase
Schuster, M., Treitschke, S., Kilaru, S., Molloy, J. E., Harmer, N. J., & Steinberg, G. (2011) · The EMBO Journal · doi:10.1038/emboj.2011.361 · Cited by 119 · see citing works
Self-citationGenetic regulation of mating and dimorphism in Ustilago maydis
Bölker, M., Genin, S., Lehmler, C., & Kahmann, R. (1995) · Canadian Journal of Botany · doi:10.1139/b95-262 · Cited by 115 · see citing works
Predicting the targeting of tail-anchored proteins to subcellular compartments in mammalian cells
Costello, J. L., Castro, I. G., Camões, F., Schrader, T. A., McNeall, D., Yang, J., Giannopoulou, E., Gomes, S. R. A. M., Pogenberg, V., Bonekamp, N. A., et al. (2017) · Journal of Cell Science · doi:10.1242/jcs.200204 · Cited by 107 · see citing works
Self-citationPeroxisomes move by hitchhiking on early endosomes using the novel linker protein PxdA
Salogiannis, J., Egan, M. J., & Reck‐Peterson, S. L. (2016) · The Journal of Cell Biology · doi:10.1083/jcb.201512020 · Cited by 104 · see citing works
A role for the human peroxisomal half-transporter ABCD3 in the oxidation of dicarboxylic acids
van Roermund, C. W. T., IJlst, L., Wagemans, T., Wanders, R. J. A., & Waterham, H. R. (2014) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/j.bbalip.2013.12.001 · Cited by 101 · see citing works
Peroxisomal Atg37 binds Atg30 or palmitoyl-CoA to regulate phagophore formation during pexophagy
Nazarko, T. Y., Ozeki, K., Till, A., Ramakrishnan, G., Lotfi, P., Yan, M., & Subramani, S. (2014) · Journal of Cell Biology · doi:10.1083/jcb.201307050 · Cited by 99 · see citing works
ACBD2/ECI2-Mediated Peroxisome-Mitochondria Interactions in Leydig Cell Steroid Biosynthesis
Fan, J., Li, X., Issop, L., Culty, M., & Papadopoulos, V. (2016) · Molecular Endocrinology · doi:10.1210/me.2016-1008 · Cited by 99 · see citing works
Chai, A., Withers, J., Koh, Y. H., Parry, K., Bao, H., Zhang, B., Budnik, V., & Pennetta, G. (2007) · Human Molecular Genetics · doi:10.1093/hmg/ddm303 · Cited by 94 · see citing works
Simm, D., Hatje, K., & Kollmar, M. (2014) · Bioinformatics · doi:10.1093/bioinformatics/btu700 · Cited by 93 · see citing works
Schuster, M., Kilaru, S., Ashwin, P., Lin, C., Severs, N. J., & Steinberg, G. (2011) · The EMBO Journal · doi:10.1038/emboj.2010.360 · Cited by 92 · see citing works
Self-citationPeroxisome Function Regulates Growth on Glucose in the Basidiomycete Fungus Cryptococcus neoformans
Idnurm, A., Giles, S. S., Perfect, J. R., & Heitman, J. (2007) · Eukaryotic Cell · doi:10.1128/ec.00214-06 · Cited by 92 · see citing works
Furuita, K., Jee, J., Fukada, H., Mishima, M., & Kojima, C. (2010) · Journal of Biological Chemistry · doi:10.1074/jbc.m109.082602 · Cited by 91 · see citing works
Lin, C., Schuster, M., Guimarães, S., Ashwin, P., Schrader, M., Metz, J., Hacker, C., Gurr, S. J., & Steinberg, G. (2016) · Nature Communications · doi:10.1038/ncomms11814 · Cited by 90 · see citing works
Self-citationSun, J., Xu, A. Q., Giraud, J., Poppinga, H., Riemensperger, T. D., Fiala, A., & Birman, S. (2018) · Frontiers in Systems Neuroscience · doi:10.3389/fnsys.2018.00006 · Cited by 89 · see citing works
Kragelund, B. B., Poulsen, K., Andersen, K. V., Baldursson, T., Krøll, J. B., Neergård, T. B., Stenvang, J., Roepstorff, P., Kristiansen, K., Poulsen, F. M., et al. (1999) · Biochemistry · doi:10.1021/bi982427c · Cited by 88 · see citing works
Yagita, Y., Shinohara, K., Abe, Y., Nakagawa, K., Al-Owain, M., Alkuraya, F. S., & Fujiki, Y. (2017) · Journal of Biological Chemistry · doi:10.1074/jbc.m116.760090 · Cited by 85 · see citing works
A role for Mitochondrial Rho GTPase 1 (MIRO1) in motility and membrane dynamics of peroxisomes
Castro, I. G., Richards, D. M., Metz, J., Costello, J. L., Passmore, J. B., Schrader, T. A., Gouveia, A., Ribeiro, D., & Schrader, M. (2018) · Traffic · doi:10.1111/tra.12549 · Cited by 84 · see citing works
Self-citationAn Inventory of Peroxisomal Proteins and Pathways in Drosophila melanogaster
Faust, J. E., Verma, A., Peng, C., & McNew, J. A. (2012) · Traffic · doi:10.1111/j.1600-0854.2012.01393.x · Cited by 79 · see citing works
Peroxisomal ACBD4 interacts with VAPB and promotes ER-peroxisome associations
Costello, J. L., Castro, I. G., Schrader, T. A., Islinger, M., & Schrader, M. (2017) · Cell Cycle · doi:10.1080/15384101.2017.1314422 · Cited by 79 · see citing works
Self-citationSmith, G. A., Lin, T.-H., Sheehan, A. E., van Naters, W. V. D. G., Neukomm, L. J., Graves, H. K., Bis‐Brewer, D. M., Zuchner, S. L., & Freeman, M. R. (2019) · Neuron · doi:10.1016/j.neuron.2019.04.017 · Cited by 73 · see citing works
Self-citationKottmeier, R., Bittern, J., Schoofs, A., Scheiwe, F., Matzat, T., Pankratz, M. J., & Klämbt, C. (2020) · Nature Communications · doi:10.1038/s41467-020-18291-1 · Cited by 71 · see citing works
The amyotrophic lateral sclerosis 8 protein, VAP, is required for ER protein quality control
Moustaqim‐Barrette, A., Qi, L. Y., Pradhan, S., Neely, G. G., Bellen, H. J., & Tsuda, H. (2013) · Human Molecular Genetics · doi:10.1093/hmg/ddt594 · Cited by 70 · see citing works
van Aalten, D. M. F., Milne, K. G., Zou, J.-Y., Kleywegt, G. J., Bergfors, T., Ferguson, M. A. J., Knudsen, J., & Jones, T. A. (2001) · Journal of Molecular Biology · doi:10.1006/jmbi.2001.4749 · Cited by 68 · see citing works
A lipophilic cation protects crops against fungal pathogens by multiple modes of action
Steinberg, G., Schuster, M., Gurr, S. J., Schrader, T. A., Schrader, M., Wood, M. E., Early, A., & Kilaru, S. (2020) · Nature Communications · doi:10.1038/s41467-020-14949-y · Cited by 65 · see citing works
Self-citationCamões, F., Islinger, M., Guimarães, S., Kilaru, S., Schuster, M., Godinho, L. F., Steinberg, G., & Schrader, M. (2014) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/j.bbamcr.2014.10.005 · Cited by 63 · see citing works
Self-citationA Drosophila model for the Zellweger spectrum of peroxisome biogenesis disorders
Mast, F. D., Li, J., Virk, M. K., Hughes, S. C., Simmonds, A. J., & Rachubinski, R. A. (2011) · Disease Models & Mechanisms · doi:10.1242/dmm.007419 · Cited by 63 · see citing works
Kors, S., Costello, J. L., & Schrader, M. (2022) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2022.895856 · Cited by 60 · see citing works
Self-citationMao, D., Lin, G., Tepe, B., Zuo, Z., Tan, K. L., Şentürk, M., Zhang, S., Arenkiel, B. R., Sardiello, M., & Bellen, H. J. (2019) · Autophagy · doi:10.1080/15548627.2019.1580103 · Cited by 58 · see citing works
Regulating peroxisome–ER contacts via the ACBD5-VAPB tether by FFAT motif phosphorylation and GSK3β
Kors, S., Hacker, C., Bolton, C., Maier, R., Reimann, L., Kitchener, E. J. A., Warscheid, B., Costello, J. L., & Schrader, M. (2021) · The Journal of Cell Biology · doi:10.1083/jcb.202003143 · Cited by 58 · see citing works
Self-citationFunctional characterisation of peroxisomal β‐oxidation disorders in fibroblasts using lipidomics
Herzog, K., Pras‐Raves, M. L., Ferdinandusse, S., Vervaart, M. A. T., Luyf, A. C. M., van Kampen, A. H. C., Wanders, R. J. A., Waterham, H. R., & Vaz, F. M. (2018) · Journal of Inherited Metabolic Disease · doi:10.1007/s10545-017-0076-9 · Cited by 57 · see citing works
The diversity of ACBD proteins – From lipid binding to protein modulators and organelle tethers
Islinger, M., Costello, J. L., Kors, S., Soupène, E., Levine, T. P., Kuypers, F. A., & Schrader, M. (2020) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/j.bbamcr.2020.118675 · Cited by 55 · see citing works
Self-citationNetwork Analyses Reveal Novel Aspects of ALS Pathogenesis
Sanhueza, M., Chai, A., Smith, C., McCray, B. A., Simpson, T. I., Taylor, J. P., & Pennetta, G. (2015) · PLoS Genetics · doi:10.1371/journal.pgen.1005107 · Cited by 53 · see citing works
Nakayama, M., Sato, H., Okuda, T., Fujisawa, N., Kono, N., Arai, H., Suzuki, E., Umeda, M., Ishikawa, H., & Matsuno, K. (2011) · PLoS ONE · doi:10.1371/journal.pone.0022984 · Cited by 47 · see citing works
Kretschmer, M., Klose, J., & Kronstad, J. W. (2012) · Eukaryotic Cell · doi:10.1128/ec.00129-12 · Cited by 45 · see citing works
Klose, J., & Kronstad, J. W. (2006) · Eukaryotic Cell · doi:10.1128/ec.00231-06 · Cited by 45 · see citing works
Deciphering the roles of acyl-CoA-binding proteins in plant cells
Lung, S., & Chye, M. (2015) · PROTOPLASMA · doi:10.1007/s00709-015-0882-6 · Cited by 42 · see citing works
Genome sequencing in persistently unsolved white matter disorders
Helman, G., Lajoie, B. R., Crawford, J., Takanohashi, A., Walkiewicz, M., Dolzhenko, E., Gross, A. M., Gainullin, V. G., Bent, S. J., Jenkinson, E. M., et al. (2020) · Annals of Clinical and Translational Neurology · doi:10.1002/acn3.50957 · Cited by 39 · see citing works
Determinants of Peroxisome Membrane Dynamics
Carmichael, R. E., & Schrader, M. (2022) · Frontiers in Physiology · doi:10.3389/fphys.2022.834411 · Cited by 38 · see citing works
Self-citationPeroxisomes in wild-type and rosy mutant Drosophila melanogaster.
Beard, M. E., & Holtzman, E. J. (1987) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.84.21.7433 · Cited by 37 · see citing works
Kilaru, S., & Steinberg, G. (2015) · Fungal Genetics and Biology · doi:10.1016/j.fgb.2015.03.017 · Cited by 37 · see citing works
Self-citationZientara‐Rytter, K., Ozeki, K. A., Nazarko, T. Y., & Subramani, S. E. (2017) · Autophagy · doi:10.1080/15548627.2017.1413521 · Cited by 33 · see citing works
Systematic multi‐level analysis of an organelle proteome reveals new peroxisomal functions
Yifrach, E., Holbrook‐Smith, D., Bürgi, J., Othman, A., Eisenstein, M., van Roermund, C. W. T., Visser, W. F., Tirosh, A., Rudowitz, M., Bibi, C., et al. (2022) · Molecular Systems Biology · doi:10.15252/msb.202211186 · Cited by 33 · see citing works
Darwisch, W., von Spangenberg, M., Lehmann, J., Singin, Ö., Deubert, G., Kühl, S., Roos, J., Horstmann, H., Körber, C., Hoppe, S., et al. (2020) · Communications Biology · doi:10.1038/s42003-020-01442-x · Cited by 32 · see citing works
Self-citationPeroxisomes and their central role in metabolic interaction networks in humans.
Wanders, R. J. A., Waterham, H. R., & Ferdinandusse, S. (2018) · Subcellular Biochemistry · doi:10.1007/978-981-13-2233-4_15 · Cited by 31 · see citing works
Protective role of the deSUMOylating enzyme SENP3 in myocardial ischemia-reperfusion injury
Rawlings, N., Lee, L. J., Nakamura, Y., Wilkinson, K. A., & Henley, J. M. (2019) · PLoS ONE · doi:10.1371/journal.pone.0213331 · Cited by 31 · see citing works
Bonekamp, N. A., Vormund, K., Jacob, R., & Schrader, M. (2010) · Experimental Cell Research · doi:10.1016/j.yexcr.2010.07.020 · Cited by 27 · see citing works
Self-citationPxdA interacts with the DipA phosphatase to regulate peroxisome hitchhiking on early endosomes
Salogiannis, J., Christensen, J. R., Songster, L. D., Aguilar-Maldonado, A., Shukla, N., & Reck‐Peterson, S. L. (2021) · Molecular Biology of the Cell · doi:10.1091/mbc.e20-08-0559 · Cited by 26 · see citing works
Bacteria, Bacteriophages, and Fungi
King, R. C. (1974) · Springer eBooks · doi:10.1007/978-1-4899-1710-2 · Cited by 26 · see citing works
Steinberg, G., & Schuster, M. (2011) · Fungal Biology Reviews · doi:10.1016/j.fbr.2011.01.008 · Cited by 24 · see citing works
Self-citationA Systematic Cell‐Based Analysis of Localization of Predicted Drosophila Peroxisomal Proteins
Baron, M. N., Klinger, C. M., Rachubinski, R. A., & Simmonds, A. J. (2016) · Traffic · doi:10.1111/tra.12384 · Cited by 24 · see citing works
Intracellular redistribution of neuronal peroxisomes in response to ACBD5 expression
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Ensheathing glia promote increased lifespan and healthy brain aging
Sheng, L., Shields, E. J., Gospočić, J., Sorida, M., Ju, L., Byrns, C. N., Carranza, F. R., Berger, S. L., Bonini, N. M., & Bonasio, R. (2023) · Aging Cell · doi:10.1111/acel.13803 · Cited by 21 · see citing works
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Barone, F. G., Urbé, S., & Clague, M. J. (2023) · Life Science Alliance · doi:10.26508/lsa.202201825 · Cited by 20 · see citing works
Bartlett, M., Nasiri, N., Pressman, R., Bademci, G., & Forghani, I. (2021) · American Journal of Medical Genetics Part A · doi:10.1002/ajmg.a.62073 · Cited by 20 · see citing works
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Self-citationDifferential roles for ACBD4 and ACBD5 in peroxisome-ER interactions and lipid metabolism.
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Self-citationKamemura, K., Chen, C., Okumura, M., Miura, M., & Chihara, T. (2021) · Genes to Cells · doi:10.1111/gtc.12835 · Cited by 15 · see citing works
Newly defined peroxisomal disease with novel <i>ACBD5</i> mutation
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Karagas, N. E., Gupta, R., Rastegari, E., Tan, K. L., Leung, H. H., Bellen, H. J., Venkatachalam, K., & Wong, C. (2022) · Journal of Neuroscience · doi:10.1523/jneurosci.2456-21.2022 · Cited by 12 · see citing works
Whole organism and tissue-specific analysis of pexophagy in Drosophila
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Kremp, M., Bittner, E., Martorana, D., Klingenberger, A., Stehlik, T., Bölker, M., & Freitag, J. (2020) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2020.00251 · Cited by 9 · see citing works
Ast, J., Bäcker, N., Bittner, E., Martorana, D., Ahmad, H., Bölker, M., & Freitag, J. (2022) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2022.858084 · Cited by 8 · see citing works
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Variables in the ACBD5 gene leading to distinct phenotypes: A case report.
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Self-citationHasturk, B. A., Cinar, Ç., Zübarioğlu, T., Demir, S. T., Cansever, M. Ş., Kıykım, E., Yiğin, A. K., Yalçınkaya, C., & Zeybek, A. Ç. A. (2024) · Molecular Syndromology · doi:10.1159/000535534 · Cited by 3 · see citing works
Clonal Imaging of Mitochondria in the Dissected Fly WingPreprint
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Self-citationLabeling of Peroxisomes for Live Cell Imaging in the Filamentous Fungus Ustilago maydis
Guimarães, S., Kilaru, S., Schrader, M., & Schuster, M. (2017) · Methods in molecular biology · doi:10.1007/978-1-4939-6937-1_13 · Cited by 1 · see citing works
Self-citationBarbero (2014) · doi:10.1016/j · Cited by 1 · see citing works
(2018) · Digital Commons@Becker (Washington University School of Medicine) · doi:10.1371/journal
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Research works printed but not linked: 2
- [85][85] L.N. Townsend, H. Clarke, D. Maddison, K.M. Jones, L. Amadio, A. Jefferson, U. Chughtai, D.M. Bis, S. Züchner, N.D. Allen, W. Van der Goes, O.M. van Naters, G.A. Smith Peters, Cdk12 maintains the integrity of adult axons by suppressing actin remodeling, Cell Death Dis. 9 (2023) 1 -12, https://doi.org/10.1038/ s41420-023-01642-4. -
- [96][96] S.C. Guimar ˜ aes, S. Kilaru, M. Schrader, M. Schuster, Labeling of peroxisomes for live cell imaging in the filamentous fungus Ustilago maydis , Methods Mol. Biol. 1595 (2017) 131 -150, https://doi.org/10.1007/978-1-4939-6937-1\_13. - · DOI
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Update on VAP, a ubiquitous signpost for the ER
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Peroxisome dynamics and inter-organelle interactions in neuronal health and disease
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Driscoll, B. E., Fountain, M. B., Gates, I. N., Abdollahi, R., Langley, A., Owens, M. B., Christensen, J. R., & Salogiannis, J. (2025) · Molecular Biology of the Cell · doi:10.1091/mbc.e25-04-0186 · Cited by 6 · see citing works
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Ferreira, V., Sarabando, J., & Ribeiro, D. (2025) · Microscopy and Microanalysis · doi:10.1093/mam/ozaf090 · Cited by 2 · see citing works
Seventy years of peroxisome research: current advances and future perspectives
Carmichael, R. E., Oeljeklaus, S., Cruz‐Zaragoza, L. D., Kumar, R., Bolton, C., Islinger, M., Kunze, M., Pata, J., Lismont, C., Silva, B. S. C., et al. (2025) · Histochemistry and Cell Biology · doi:10.1007/s00418-024-02349-y · Cited by 1 · see citing works
Self-citationNeuro-glial lipid imbalance in a Drosophila model of amyotrophic lateral sclerosis 8
Garg, L., Chaplot, K., Kuppili, G., Tendulkar, S., Joseph, J., Kamat, S. S., & Ratnaparkhi, G. S. (2026) · Acta Neuropathologica Communications · doi:10.1186/s40478-026-02391-y
Schrader, M., Kim, P. K., & Carmichael, R. E. (2026) · Contact · doi:10.1177/25152564261471034
Self-citationStudying Peroxisomes in Ustilago maydis: A Defined Workflow
Bäcker, N., Freitag, D., Bange, G., Klatt, J. M., & Freitag, J. (2026) · Methods in molecular biology · doi:10.1007/978-1-0716-5364-7_34
- Authors
- Kors, S., Schuster, M., Maddison, D. C., Kilaru, S., Schrader, T. A., Costello, J. L., Islinger, M., Smith, G. A., & Schrader, M.
- Affiliations
- University of Exeter (GB)
- Cardiff University (GB)
- Heidelberg University (DE)
- Corresponding author
- Schrader, Michael
- ORCID
- Venue
- Biochimica et Biophysica Acta (BBA) - Molecular Cell Research
- Publisher
- Elsevier BV
- Published
- 2024-12
- Volume
- 1871
- Issue
- 8
- Pages
- 119843
- ISSN
- 0167-4889 (ISSN-L)
- OpenAlex
- W4402450038
- Cited by
- 12
- References
- 110
- Citations by year
- 2026: 4 · 2025: 8
- FWCI
- 1.93
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Biochimica et Biophysica Acta (BBA) - Molecular Cell Research (publishedVersion, cc-by)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- ORCA Online Research @Cardiff (Cardiff University) (acceptedVersion)
- Figshare (submittedVersion, cc-by)
- Open Research Exeter (University of Exeter) (submittedVersion, cc-by)
- Apollo (University of Cambridge) (submittedVersion, cc-by)
- Funders
- Biotechnology and Biological Sciences Research Council (BB/T002255/1, BB/W015420/1, BB/N01541X/1)
- German Center for Cardiovascular Disease (81X2500211)
- UK Research and Innovation Medical Research Council (MC_PC_16030/1)
- German Research Foundation (397476530)
- UK Research and Innovation (MR/T019409/1)
- The Royal Society (RGS\\R2\\192378)
- Leverhulme Trust (RPG-2020-369)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer, Lipids, and Metabolism
- Keywords
- FFAT motif, VAP proteins, peroxisomal beta-oxidation, peroxisome biogenesis, lipid metabolism, Drosophila melanogaster, Ustilago maydis, lipid droplets, mitochondrial membrane potential, evolutionary conservation, protein domain structure, nomenclature
- MeSH terms
- Animals, Basidiomycota, Drosophila melanogaster, Endoplasmic Reticulum, Female, Humans, Male, Membrane Proteins, Phylogeny, Peroxisomes, Diazepam Binding Inhibitor, Drosophila Proteins, Adaptor Proteins, Signal Transducing, Lipid Metabolism
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