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Intestinal dysbiosis mediates cognitive impairment via the intestine and brain NLRP3 inflammasome activation in chronic sleep deprivation
Abstract
Growing evidence suggests the involvement of the microbiota-gut-brain axis in cognitive impairment induced by sleep deprivation (SD), however how the microbiota-gut-brain axis work remains elusive. Here, we discovered that chronic SD induced intestinal dysbiosis, activated NLRP3 inflammasome in the colon and brain, destructed intestinal/blood-brain barrier, and impaired cognitive function in mice. Transplantation of "SD microbiota" could almost mimic the pathological and behavioral changes caused by chronic SD. Furthermore, all the behavioral and pathological abnormalities were practically reversed in chronic sleep-deprived NLRP3-/- mice. Regional knockdown NLRP3 expression in the gut and hippocampus, respectively. We observed that down-regulation of NLRP3 in the hippocampus inhibited neuroinflammation, and ameliorated synaptic dysfunction and cognitive impairment induced by chronic SD. More intriguingly, the down-regulation of NLRP3 in the gut protected the intestinal barrier, attenuated the levels of peripheral inflammatory factors, down-regulated the expression of NLRP3 in the brain, and improved cognitive function in chronic SD mice. Our results identified gut microbiota as a driver in chronic SD and highlighted the NLRP3 inflammasome as a key regulator within the microbiota-gut-brain axis.
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In the citing paper
“Chronic SD triggers gut microbiota alteration, leading to reduced mucus thickness and reduced levels of TJ proteins in mice colons, via NLRP3 inflammasome activation.”
From Microbiota–gut–brain axis and its therapeutic applications in neurodegenerative diseases (2024) · cites this paper as [646]
In the citing paper
“sleep deprivation (SD) induces gut dysbiosis and intestinal barrier disruption, leading to microglial activation and cognitive decline”
From Microbiota–gut–brain axis and its therapeutic applications in neurodegenerative diseases (2024) · cites this paper as [646]
In the citing paper
“FMT from chronically sleep-deprived mice to control mice largely recapitulated the pathological changes observed in chronic SD.”
From Microbiota–gut–brain axis and its therapeutic applications in neurodegenerative diseases (2024) · cites this paper as [646]
In the citing paper
“This is accompanied by impaired BBB integrity, NLRP3 activation in the brain, microglial activation, and cognitive impairment.”
From Microbiota–gut–brain axis and its therapeutic applications in neurodegenerative diseases (2024) · cites this paper as [646]
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Self-citationLuo, X., Jin, S., Fang, Y., Zhao, Q., Xie, H., & Han, L. (2026) · Metabolites · doi:10.3390/metabo16060376
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Self-citationEnterochromaffin Cells Drive Anxiety-Like Behaviors in Sleep-Deprived Mice
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Do microbiota rhythms influence cognitive performance? - A narrative review
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Metadata
- Authors
- Zhao, N., Chen, Q.-G., Chen, X., Liu, X.-T., Geng, F., Zhu, M.-M., Yan, F.-L., Zhang, Z.-J., & Ren, Q.-G.
- Affiliations
- Zhongda Hospital Southeast University (CN)
- Venue
- Brain, Behavior, and Immunity
- Publisher
- Elsevier BV
- Published
- 2023-02
- Volume
- 108
- Pages
- 98-117
- ISSN
- 0889-1591 (ISSN-L)
- OpenAlex
- W4309880386
- Cited by
- 105
- References
- 59
- Citations by year
- 2026: 37 · 2025: 34 · 2024: 28 · 2023: 6
- FWCI
- 5.63
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Brain Behavior and Immunity (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Inflammasome and immune disorders
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Keywords
- gut dysbiosis, microbiota-gut-brain axis, NLRP3 inflammasome, chronic sleep deprivation, cognitive impairment, blood-brain barrier, intestinal barrier, neuroinflammation, synaptic dysfunction, hippocampus, gut microbiota transplantation, peripheral inflammation
- MeSH terms
- NLR Family, Pyrin Domain-Containing 3 Protein, Animals, Hippocampus, Intestines, Sleep Deprivation, Mice, Inflammasomes, Cognitive Dysfunction, Dysbiosis
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