Student or educator? Sign up with your school email and get 6 months of Pro free.
CoChat
Sign Up
Cited by 85

The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.

Aspirin reduces endothelial cell senescence

Bode-Böger, Stefanie M., Martens-Lobenhoffer, Jens, Täger, Michael, Schröder, Henning, Scalera, Fortunato·2005·Biochemical and Biophysical Research Communications
DOI 10.1016/j.bbrc.2005.07.014
Open this paper in CoChat — free account

Abstract

We report here the effect of aspirin on the onset of replicative senescence. Endothelial cells that were cultured until cumulative population doublings 40 showed clear signs of aging. Incubation with aspirin inhibited senescence-associated beta-galactosidase activity and increased telomerase activity. Along with the delayed onset of senescence, aspirin decreased reactive oxygen species and increased nitric oxide (NO) and cGMP levels. Furthermore, aspirin reduced the elaboration of asymmetric dimethylarginine (ADMA), an endogenous inhibitor of NO synthase, and up-regulated the activity of dimethylarginine dimethylaminohydrolase, the enzyme that degrades ADMA. These effects were specific in that other nonsteroidal anti-inflammatory drugs, such as ibuprofen or acetaminophen, did not prevent the onset of endothelial senescence. The NO synthase inhibitor l-NAME, but not its inactive d-enantiomer, led to complete inhibition of aspirin-delayed senescence. These findings demonstrate that aspirin delays the onset of endothelial senescence by preventing a decrease in NO formation/generation. This might provide a therapeutic strategy aimed at blocking aging-induced NO inhibition.

Overview

Integrity signals

Registry

Crossref · 10.1016/j.bbrc.2005.07.014

Cites

2 of 2 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Research works printed but not linked: 31

  1. Hayflick, 1961, The serial cultivation of human diploid cell strains, Exp. Cell Res., 25, 585, 10.1016/0014-4827(61)90192-6 · DOI · Paper page
  2. Matsushita, 2001, eNOS activity is reduced in senescent human endothelial cells: preservation by hTERT immortalization, Circ. Res., 89, 793, 10.1161/hh2101.098443 · DOI
  3. Scalera, 2004, Endogenous nitric oxide synthesis inhibitor asymmetric dimethyl l-arginine accelerates endothelial cell senescence, Arterioscler. Thromb. Vasc. Biol., 24, 1816, 10.1161/01.ATV.0000141843.77133.fc · DOI
  4. Minamino, 2002, Endothelial cell senescence in human atherosclerosis. Role of telomere in endothelial dysfunction, Circulation, 105, 1541, 10.1161/01.CIR.0000013836.85741.17 · DOI · Paper page
  5. Zoccali, 2001, Plasma concentration of asymmetrical dimethylarginine and mortality in patients with end-stage renal disease: a prospective study, Lancet, 358, 2113, 10.1016/S0140-6736(01)07217-8 · DOI · Paper page
  6. Kielstein, 2003, Asymmetric dimethylarginine, blood pressure, and renal perfusion in elderly subjects, Circulation, 107, 1891, 10.1161/01.CIR.0000060496.23144.A7 · DOI
  7. van der Loo, 2000, Enhanced peroxynitrite formation is associated with vascular aging, J. Exp. Med., 192, 1731, 10.1084/jem.192.12.1731 · DOI
  8. Serrano, 2004, Telomeres and cardiovascular disease. Does size matter?, Circ. Res., 94, 575, 10.1161/01.RES.0000122141.18795.9C · DOI
  9. Vasa, 2000, Nitric oxide activates telomerase and delays endothelial cell senescence, Circ. Res., 87, 540, 10.1161/01.RES.87.7.540 · DOI
  10. Xu, 2000, Homocysteine accelerates endothelial cell senescence, FEBS Lett., 470, 20, 10.1016/S0014-5793(00)01278-3 · DOI
  11. Haendeler, 2004, Antioxidants inhibit nuclear export of telomerase reverse transcriptase and delay replicative senescence of endothelial cells, Circ. Res., 94, 768, 10.1161/01.RES.0000121104.05977.F3 · DOI
  12. Patrono, 1994, Aspirin as an antiplatelet drug, N. Engl. J. Med., 330, 1287, 10.1056/NEJM199405053301808 · DOI
  13. Podhaisky, 1997, Aspirin protects endothelial cells from oxidative stress—possible synergism with vitamin E, FEBS Lett., 417, 349, 10.1016/S0014-5793(97)01307-0 · DOI
  14. Wu, 2002, Antioxidative properties of acetylsalicylic acid on vascular tissue from normotensive and spontaneously hypertensive rats, Circulation, 105, 387, 10.1161/hc0302.102609 · DOI
  15. Grosser, 2003, Aspirin protects endothelial cells from oxidant damage via nitric oxide-cGMP pathway, Arterioscler. Thromb. Vasc. Biol., 23, 1345, 10.1161/01.ATV.0000083296.57581.AE · DOI
  16. Taubert, 2004, Aspirin induces nitric oxide release from vascular endothelium: a novel mechanism of action, Br. J. Pharmacol., 143, 159, 10.1038/sj.bjp.0705907 · DOI
  17. Dragomir, 2004, Aspirin rectifies calcium homeostasis, decreases reactive oxygen species, and increases NO production in high glucose-exposed human endothelial cells, J. Diabetes Complications, 5, 289, 10.1016/j.jdiacomp.2004.03.003 · DOI
  18. Dimri, 1995, A biomarker that identifies senescent human cells in culture and in aging skin in vivo, Proc. Natl. Acad. Sci. USA, 92, 9363, 10.1073/pnas.92.20.9363 · DOI · Paper page
  19. Tsikas, 1994, Quantification of nitrite and nitrate in human urine and plasma as pentafluorobenzyl derivatives by gas chromatography-mass spectrometry using their 15N-labelled analogs, J. Chromatogr. B, 661, 185, 10.1016/0378-4347(94)00374-2 · DOI
  20. Martens-Lobenhoffer, 2004, Determination of arginine and asymmetric dimethylarginine (ADMA) in human plasma by liquid chromatography/mass spectrometry with the isotope dilution technique, J. Mass. Spectrom., 39, 1287, 10.1002/jms.684 · DOI
  21. Lin, 2002, Impaired nitric oxide synthase pathway in diabetes mellitus. Role of asymmetric dimethylarginine and dimethylarginine dimethylaminohydrolase, Circulation, 106, 987, 10.1161/01.CIR.0000027109.14149.67 · DOI
  22. Dietzmann, 2002, Thiol-inducing and immunoregulatory effects of flavonoids in peripheral blood mononuclear cells from patients with end-stage diabetic nephropathy, Free Radic. Biol. Med., 33, 1347, 10.1016/S0891-5849(02)01043-2 · DOI
  23. Finkel, 2000, Oxidants, oxidative stress and the biology of ageing, Nature, 408, 239, 10.1038/35041687 · DOI · Paper page
  24. Kurz, 2004, Chronic oxidative stress compromises telomere integrity and accelerates the onset of senescence in human endothelial cells, J. Cell Sci., 117, 2417, 10.1242/jcs.01097 · DOI · Paper page
  25. Oberle, 1998, Aspirin increases ferritin synthesis in endothelial cells. A novel antioxidant pathway, Circ. Res., 82, 1016, 10.1161/01.RES.82.9.1016 · DOI
  26. Grosser, 2003, Heme oxygenase-1 induction may explain the antioxidant profile of aspirin, Biochem. Biophys. Res. Commun., 308, 956, 10.1016/S0006-291X(03)01504-3 · DOI
  27. Nascimento-Silva, 2005, Novel lipid mediator aspirin-triggered lipoxin A4 induces heme oxygenase-1 on endothelial cells, Am. J. Physiol. Cell Physiol., 10.1152/ajpcell.00045.2005 · DOI
  28. Paul-Clark, 2004, 15-epi-Lipoxin A4-mediated induction of nitric oxide explains how aspirin inhibits acute inflammation, J. Exp. Med., 200, 69, 10.1084/jem.20040566 · DOI
  29. Ito, 1999, Novel mechanism for endothelial dysfunction. Dysregulation of dimethylarginine dimethylaminohydrolase, Circulation, 99, 3092, 10.1161/01.CIR.99.24.3092 · DOI
  30. Stühlinger, 2001, Homocysteine impairs the nitric oxide synthase pathway. Role of asymmetric dimethylarginine, Circulation, 104, 2569, 10.1161/hc4601.098514 · DOI
  31. Scalera, 2005, Erythropoietin increases asymmetric dimethylarginine in endothelial cells: role of dimethylarginine dimethylaminohydrolase, J. Am. Soc. Nephrol., 16, 892, 10.1681/ASN.2004090735 · DOI

Cited by

See all 85

Metadata

Authors
Bode-Böger, S. M., Martens-Lobenhoffer, J., Täger, M., Schröder, H., & Scalera, F.
Venue
Biochemical and Biophysical Research Communications
Publisher
Elsevier BV
Published
2005-09
Volume
334
Issue
4
Pages
1226-1232
ISSN
0006-291X
DOI
10.1016/j.bbrc.2005.07.014
Cited by
85
References
2
Language
en
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review