β-adaptin: Key molecule for microglial scavenger receptor function under oxidative stress
Abstract
Scavenger receptors internalize chemically modified low density lipoprotein particles (ac-LDL) and other ligands through the process of receptor-mediated endocytosis. During this investigation using amyloid-beta as a natural ligand for the SR, we studied under a ligand-induced oxidative stress condition, changes in protein expression of several adaptor proteins important in the organization of the endocytic machinery in microglia and macrophages. Differential expression experiments of beta-adaptin, alpha-adaptin, SR-AI, and SR-BI in RAW (macrophages) and EOC (microglia) cells were performed according to dosage and exposure time to amyloid-beta. Our results show that according to dosage, amyloid-beta produces an oxidative stress state that importantly affects the availability of beta-adaptin. Under these conditions, RT-PCR assays show that beta-adaptin mRNA is normally synthesized, reason why protein translation or protein structure of beta-adaptin might be altered. These observations might have impact in the understanding of the mechanisms microglia employ to process amyloid-beta in the brain.
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Aβ binding to scavenger receptors induces oxidative stress, which may impair AP1B1-dependent internalization of Aβ.
“work with a microglial cell line suggests that Aβ binding to scavenger receptors induces oxidative stress, which then may impair AP1B1-dependent internalization of the Aβ (82).”
From <i>Trem2</i> promotes anti-inflammatory responses in microglia and is suppressed under pro-inflammatory conditions (2020) · cites this paper as [82]
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“Further work is required to understand the function of AP1B1 in microglia, although work with a microglial cell line suggests that Aβ binding to scavenger receptors induces oxidative stress, which then may impair AP1B1-dependent internalization of the Aβ (82).”
Background
- Authors
- Manzano-León, N., Delgado-Coello, B., Guaderrama-Díaz, M., & Mas-Oliva, J.
- Affiliations
- Universidad Nacional Autónoma de México (MX)
- Venue
- Biochemical and Biophysical Research Communications
- Publisher
- Elsevier BV
- Published
- 2006-12
- Volume
- 351
- Issue
- 3
- Pages
- 588-594
- ISSN
- 0006-291X (ISSN-L)
- OpenAlex
- W2065325633
- Cited by
- 14
- References
- 29
- Citations by year
- 2020: 1 · 2018: 1 · 2017: 1 · 2015: 3 · 2014: 3 · 2013: 1 · 2012: 2
- FWCI
- 0.22
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Biochemical and Biophysical Research Communications (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Consejo Nacional de Ciencia y Tecnología (38551-N)
- Topics
- Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Keywords
- microglia, scavenger receptors, oxidative stress, amyloid-beta, receptor-mediated endocytosis, alpha-adaptin, scavenger receptor class B type I, macrophages, adaptor proteins, amyloid-beta processing
- MeSH terms
- Animals, Cell Line, Dose-Response Relationship, Drug, Endocytosis, Amyloid beta-Peptides, Microglia, Oxidative Stress, Adaptor Protein Complex beta Subunits, Receptors, Scavenger, Mice
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