A comparative review of cell culture systems for the study of microglial biology in Alzheimer’s disease
Open accessAbstract
Over the past two decades, it has become increasingly apparent that Alzheimer’s disease neuropathology is characterized by activated microglia (brain resident macrophages) as well as the classic features of amyloid plaques and neurofibrillary tangles. The intricacy of microglial biology has also become apparent, leading to a heightened research interest in this particular cell type. Over the years a number of different microglial cell culturing techniques have been developed to study either primary mammalian microglia, or immortalized cell lines. Each microglial system has advantages and disadvantages and should be selected for its appropriateness in a particular research context. This review summarizes several of the most common microglial cell culture systems currently being employed in Alzheimer’s research including primary microglia; BV2 and N9 retroviral immortalized microglia; human immortalized microglia (HMO6); and spontaneously immortalized rodent microglial lines (EOC lines and HAPI cells). Particularities of cell culture requirements and characteristics of microglial behavior, especially in response to applied inflammogen stimuli, are compared and discussed across these cell types.
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Differences between immortalized cell lines and primary murine microglia are not insignificant.
“However, several reports have indicated that differences between immortalized cell lines and primary murine microglia are not insignificant [83,84].”
From Lipoprotein Lipase Regulates Microglial Lipid Droplet Accumulation (2021) · cites this paper as [84]
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- Authors
- Stansley, B., Post, J., & Hensley, K.
- Affiliations
- University of Toledo Medical Center (US)
- Corresponding author
- Hensley, Kenneth
- ORCID
- Stansley, Branden
- Venue
- Journal of Neuroinflammation
- Published
- 2012-05-31
- Volume
- 9
- Issue
- 1
- Pages
- 115-115
- ISSN
- 1742-2094 (ISSN-L)
- PMC
- PMC3407712
- OpenAlex
- W2164594351
- Cited by
- 419
- References
- 40
- Citations by year
- 2026: 29 · 2025: 27 · 2024: 27 · 2023: 32 · 2022: 49 · 2021: 46 · 2020: 42 · 2019: 30 · 2018: 25 · 2017: 32 · 2016: 29 · 2015: 16 · 2014: 21 · 2013: 12 · 2012: 2
- FWCI
- 8.22
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Journal of Neuroinflammation (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Funders
- National Institutes of Health (5r21ns066279-03)
- National Institute on Aging (R01AG031553)
- National Institute on Drug Abuse (DA7606, R01DA007606)
- National Institute of Neurological Disorders and Stroke (R21-NS066279)
- Topics
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
- Life Sciences › Neuroscience › Neurology › Neurological Disease Mechanisms and Treatments
- Keywords
- microglia, Alzheimer's disease, primary microglia, BV-2 cells, N9 cells, microglial activation, neuroinflammation, amyloid plaques, neurofibrillary tangles
- MeSH terms
- Alzheimer Disease, Animals, Cell Line, Transformed, Cells, Cultured, Humans, Microglia, Cell Culture Techniques
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