Cited by 133Developmental Biology and Gene RegulationUbiquitin and proteasome pathwaysHeat shock proteins research

Targeted deletion of p97 (VCP/CDC48) in mouse results in early embryonic lethality

Müller, J.M.M., Deinhardt, K., Rosewell, I., Warren, G., Shima, D.T.·2007·Biochemical and Biophysical Research Communications

Abstract

The highly conserved AAA ATPase p97 (VCP/CDC48) has well-established roles in cell cycle progression, proteasome degradation and membrane dynamics. Gene disruption in Saccromyces cerevisiae, Drosophila melanogaster and Trypanosoma brucei demonstrated that p97 is essential in unicellular and multicellular organisms. To explore the requirement for p97 in mammalian cell function and embryogenesis, we disrupted the p97 locus by gene targeting. Heterozygous p97+/- mice were indistinguishable from their wild-type littermates, whereas homozygous mutants did not survive to birth and died at a peri-implantation stage. These results show that p97 is an essential gene for early mouse development.

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Crossref · 10.1016/j.bbrc.2006.12.206

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Authors
Müller, J. M. M., Deinhardt, K., Rosewell, I., Warren, G., & Shima, D. T.
Affiliations
  • Cancer Research UK (GB)
  • Yale University (US)
Corresponding author
Shima, D.T.
Venue
Biochemical and Biophysical Research Communications
Publisher
Elsevier BV
Published
2007-03
Volume
354
Issue
2
Pages
459-465
ISSN
0006-291X (ISSN-L)
OpenAlex
W2051152369
Cited by
133
References
35
Citations by year
2026: 4 · 2025: 1 · 2024: 2 · 2023: 5 · 2022: 7 · 2021: 12 · 2020: 5 · 2019: 8 · 2018: 11 · 2017: 6 · 2016: 9 · 2015: 10 · 2014: 7 · 2013: 6 · 2012: 14
FWCI
2.52
Type
journal article
Language
en
Open access
closed
Locations
  • Biochemical and Biophysical Research Communications (publishedVersion)
  • PubMed (publishedVersion)
  • UCL Discovery (University College London) (submittedVersion)
Funders
Cancer Research UK
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ubiquitin and proteasome pathways
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Heat shock proteins research
Keywords
VCP, Cdc48, AAA+ ATPase, embryonic lethality, gene targeting, mouse mutants, peri-implantation development, embryonic development, essential genes, proteasomal degradation, membrane dynamics
MeSH terms
Valosin Containing Protein, Adenosine Triphosphatases, Animals, Breeding, Female, Heterozygote, Homozygote, Male, Mice, Inbred CBA, Mice, Inbred C57BL, Nuclear Proteins, Embryo Implantation, Gene Deletion, Mice, Knockout, Gene Targeting, Cell Cycle Proteins, Embryo Loss, Mice, Embryonic Stem Cells

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