The DOI matches its Crossref record; 24 of the 24 references it cites were found in a registry or an index.
Lipid rafts are involved in SARS-CoV entry into Vero E6 cells
Open accessAbstract
Lipid rafts often serve as an entry site for certain viruses. Here, we report that lipid rafts in Vero E6 cells are involved in the entry of severe acute respiratory syndrome coronavirus (SARS-CoV). Infectivity assay showed the integrity of lipid rafts was required for productive infection of pseudotyped SARS-CoV. Depletion of plasma membrane cholesterol with MβCD relocalized raft-resident marker caveolin-1 as well as SARS-CoV receptor ACE2 to a nonraft environment, but did not significantly change the surface expression of ACE2. MβCD-treatment inhibited infectivity of pseudotyped SARS-CoV by 90%. Biochemical fractionation and confocal imaging confirmed that ACE2 colocalized with raft-resident markers. Furthermore, an ectodomain of SARS-CoV S protein (S1188HA) could associate with lipid rafts after binding to its receptor, and colocalize with raft-resident marker ganglioside GM1. The binding of S1188HA was not affected by depleting plasma membrane cholesterol. Taken together, our results support that lipid rafts serve as an entry port for SARS-CoV.
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Caveolar endocytosis of simian virus 40 reveals a new two-step vesicular-transport pathway to the ER
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Cited by
Single-Cell RNA Expression Profiling of ACE2, the Receptor of SARS-CoV-2
Zhao, Y., Zhao, Z., Wang, Y., Zhou, Y., Ma, Y., & Zuo, W. (2020) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/rccm.202001-0179le · Cited by 916
Single-cell RNA expression profiling of ACE2, the receptor of SARS-CoV-2
Zhao, Y., Zhao, Z., Wang, Y., Zhou, Y., Ma, Y., & Zuo, W. (2020) · doi:10.1101/2020.01.26.919985 · Cited by 860
Fantini, J., Di Scala, C., Chahinian, H., & Yahi, N. (2020) · International Journal of Antimicrobial Agents · doi:10.1016/j.ijantimicag.2020.105960 · Cited by 561
Duan, Y., Gong, K., Xu, S., Zhang, F., Meng, X., & Han, J. (2022) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-022-01125-5 · Cited by 538
ACE2: Evidence of role as entry receptor for SARS-CoV-2 and implications in comorbidities
Zamorano Cuervo, N., & Grandvaux, N. (2020) · eLife · doi:10.7554/elife.61390 · Cited by 313
Metadata
- Authors
- Lu, Y., Liu, D. X., & Tam, J. P.
- Venue
- Biochemical and Biophysical Research Communications
- Publisher
- Elsevier BV
- Published
- 2008-05
- Volume
- 369
- Issue
- 2
- Pages
- 344-349
- ISSN
- 0006-291X (ISSN-L)
- OpenAlex
- W2051062943
- Cited by
- 269
- References
- 24
- FWCI
- 2.41
- Type
- journal article
- Language
- en
- Open access
- green
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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