Secreted phospholipases A2 are differentially expressed and epigenetically silenced in human breast cancer cells
Abstract
Secreted phospholipases A2 (sPLA2s) have recently been associated with several cancers, but their role in breast cancer is unknown. Here we demonstrate that mRNA expression of group IIA, III and X sPLA2s differs both in vivo in tumour biopsies and in breast cancer cells in vitro. Their expression is differentially regulated by DNA methylation and histone acetylation and, significantly, all three genes are silenced in aggressive triple negative cells due to both mechanisms. The transcription start site promoter region and the upstream CpG islands, exclusive to the group X sPLA2 gene, have variable roles in the regulation of sPLA2 expression. Our results suggest that the differential expression of hGIIA, hGIII and hGX sPLA2s in breast cancer cells is a consequence of various degrees of epigenetic silencing due to DNA hypermethylation and histone deacetylation.
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Aberrant expression of human group IIA, III and X sPLA2s has been associated with the pathology of colorectal, breast, gastric, oesophageal, ovarian and prostate cancers, providing the potential for phospholipase to be designed as a stimulator in releasing drugs responsively.
“Secretory phospholipase A2 (sPLA2) is the most studied, aberrant expression of human group IIA (hGIIA), III (hGIII) and X (hGX) sPLA2s has been associated with the pathology of colorectal, breast, gastric, oesophageal, ovarian and prostate cancers, which provide the potential for phospholipase to be designed as a stimulator in releasing drugs responsively.155,156”
From Smart nanoparticles for cancer therapy (2023) · cites this paper as [156]
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Smart nanoparticles for cancer therapy
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Self-citation
- Authors
- Brglez, V., Pucer, A., Pungerčar, J., Lambeau, G., & Petan, T.
- Affiliations
- Jožef Stefan Institute (SI)
- Corresponding author
- Petan, Toni
- Venue
- Biochemical and Biophysical Research Communications
- Publisher
- Elsevier BV
- Published
- 2014-02
- Volume
- 445
- Issue
- 1
- Pages
- 230-235
- ISSN
- 0006-291X (ISSN-L)
- OpenAlex
- W2052419474
- Cited by
- 35
- References
- 24
- Citations by year
- 2025: 3 · 2024: 3 · 2023: 8 · 2022: 2 · 2021: 3 · 2020: 5 · 2019: 2 · 2017: 2 · 2016: 2 · 2015: 3 · 2014: 2
- FWCI
- 1.23
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biochemical and Biophysical Research Communications (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Fondation ARC pour la Recherche sur le Cancer
- Javna Agencija za Raziskovalno Dejavnost RS
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer, Lipids, and Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Keywords
- secretory phospholipase A2, breast cancer, epigenetic silencing, DNA methylation, histone acetylation, histone deacetylation, triple-negative breast cancer, CpG islands, promoter regulation, gene expression regulation
- MeSH terms
- Decitabine, Azacitidine, Breast Neoplasms, Cell Line, Female, Humans, Hydroxamic Acids, Promoter Regions, Genetic, Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Neoplastic, CpG Islands, DNA Methylation, Reverse Transcriptase Polymerase Chain Reaction, Gene Expression Profiling, Epigenesis, Genetic, Cell Line, Tumor, Group II Phospholipases A2, Group X Phospholipases A2, Group III Phospholipases A2, MCF-7 Cells
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