Adiponectin is partially associated with exosomes in mouse serum
Open accessAbstract
Exosomes are membrane vesicles 30-120 nm in diameter that are released by many cell types and carry a cargo of proteins, lipids, mRNA, and microRNA. Cultured adipocytes reportedly release exosomes that may play a role in cell-to-cell communication during the development of metabolic diseases. However, the characteristics and function of exosomes released from adipocytes in vivo remain to be elucidated. Clearly, adipocyte-derived exosomes could exist in the circulation and may be associated with adipocyte-specific proteins such as adipocytokines. We isolated exosomes from serum of mice by differential centrifugation and analyzed adiponectin, leptin, and resistin in the exosome fraction. Western blotting detected adiponectin but no leptin and only trace amounts of resistin in the exosome fraction. The adiponectin signal in the exosome fraction was decreased by proteinase K treatment and completely quenched by a combination of proteinase K and Triton X-100. Quantitative ELISA showed that the exosome fraction contains considerable amounts of adiponectin, but not leptin or resistin. The concentration of adiponectin in the serum and the ratio of adiponectin to total protein in the exosome fraction were lower in obese mice than in lean mice. These results suggest that a portion of adiponectin exists as a transmembrane protein in the exosomes in mouse serum. We propose adiponectin as a marker of exosomes released from adipocytes in vivo.
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Cartonectin is a new cytokine, a paralog of adiponectin, contained as a transmembrane protein in adipocyte-released EVs and in EVs isolated from serum.
“Cartonectin is a new cytokine, paralog of adiponectin contained as a transmembrane protein in adipocyte-released EVs and in EVs isolated from serum (Phoonsawat et al., 2014).”
From Skeletal Muscle-Released Extracellular Vesicles: State of the Art (2019) · cites this paper as Phoonsawat et al., 2014
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The crosstalk: exosomes and lipid metabolism
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Skeletal Muscle-Released Extracellular Vesicles: State of the Art
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- Authors
- Phoonsawat, W., Aoki-Yoshida, A., Tsuruta, T., & Sonoyama, K.
- Affiliations
- Hokkaido University (JP)
- National Agriculture and Food Research Organization (JP)
- Corresponding author
- Sonoyama, Kei
- Venue
- Biochemical and Biophysical Research Communications
- Publisher
- Elsevier BV
- Published
- 2014-06
- Volume
- 448
- Issue
- 3
- Pages
- 261-266
- ISSN
- 0006-291X (ISSN-L)
- OpenAlex
- W2063546021
- Cited by
- 99
- References
- 31
- Citations by year
- 2026: 4 · 2025: 5 · 2024: 9 · 2023: 13 · 2022: 2 · 2021: 13 · 2020: 10 · 2019: 9 · 2018: 12 · 2017: 9 · 2016: 5 · 2015: 6 · 2014: 2
- FWCI
- 3.01
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Biochemical and Biophysical Research Communications (acceptedVersion)
- PubMed (publishedVersion)
- Institutional Repositories DataBase (IRDB) (submittedVersion)
- Hokkaido University Collection of Scholarly and Academic Papers (Hokkaido University) (submittedVersion)
- Funders
- Ministry of Education, Culture, Sports, Science and Technology (23380069)
- Mishima Kaiun Memorial Foundation
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Keywords
- adiponectin, exosomes, adipocytes, serum exosomes, leptin, resistin, adipokines, transmembrane proteins, obesity, mouse serum
- MeSH terms
- Animals, Male, Mice, Inbred C57BL, Mice, Obese, Molecular Weight, Obesity, Biomarkers, Adipocytes, Leptin, Mice, Adiponectin, Resistin, Exosomes, Tetraspanin 30
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