Cited by 43Phytoestrogen effects and researchEstrogen and related hormone effectsNatural Compounds in Disease Treatment

Estrogen receptor-independent inhibition of tumor necrosis factor-α gene expression by phytoestrogen equol is mediated by blocking nuclear factor-κB activation in mouse macrophages

Kang, Jong Soon, Yoon, Yeo Dae, Han, Mi Hwa, Han, Sang-Bae, Lee, Kiho, Kang, Moo Rim, Moon, Eun-Yi, Jeon, Young Jin +2 more·2005·Biochemical Pharmacology

Abstract

Equol has been suggested to possess protective effects on bone. However, the underlying mechanism of osteoprotective effect of equol has not been fully understood. In the present study, we examined the effect of equol on tumor necrosis factor-alpha (TNF-alpha) gene expression to elucidate a possible mechanism by which equol exerts osteoprotective effect. In vivo administration of equol inhibited TNF-alpha production by peritoneal macrophages isolated from LPS-treated mice. Equol also dose-dependently inhibited TNF-alpha production and TNF-alpha mRNA expression in LPS-stimulated mouse macrophages. Pretreatment of cells with ICI 182.780, an estrogen receptor antagonist, had no effect on the inhibitory efficacy of equol on LPS-induced TNF-alpha production. Further study demonstrated that equol inhibited NF-kappaB DNA binding and NF-kappaB-dependent reporter gene expression in activated RAW 264.7 cells. Moreover, equol blocked degradation of IkappaBalpha and IkappaBbeta and nuclear translocation of p65 subunit of NF-kappaB in activated RAW 264.7 cells. These results suggest that the inhibitory effect of equol on TNF-alpha expression is mediated, at least in part, by blocking NF-kappaB activation and the inhibition of TNF-alpha expression by equol might be involved in its osteoprotective effect.

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Crossref · 10.1016/j.bcp.2005.10.009

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Authors
Kang, J. S., Yoon, Y. D., Han, M. H., Han, S.-B., Lee, K., Kang, M. R., Moon, E.-Y., Jeon, Y. J., Park, S.-K., & Kim, H. M.
Affiliations
  • Korea Research Institute of Bioscience and Biotechnology (KR)
  • Chosun University (KR)
Corresponding author
  • Park, Song-Kyu
  • Kim, Hwan Mook
Venue
Biochemical Pharmacology
Publisher
Elsevier BV
Published
2005-12
Volume
71
Issue
1-2
Pages
136-143
ISSN
0006-2952 (ISSN-L)
OpenAlex
W2032853997
Cited by
43
References
53
Citations by year
2026: 1 · 2025: 1 · 2024: 4 · 2023: 2 · 2022: 2 · 2021: 1 · 2020: 1 · 2019: 3 · 2018: 1 · 2017: 1 · 2016: 4 · 2014: 1 · 2012: 3
FWCI
2.67
Type
journal article
Language
en
Open access
closed
Locations
  • Biochemical Pharmacology (publishedVersion)
  • PubMed (publishedVersion)
  • KRIBB Repository (submittedVersion)
Topics
  • Health Sciences › Medicine › Pathology and Forensic Medicine › Phytoestrogen effects and research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
  • Health Sciences › Medicine › Complementary and alternative medicine › Natural Compounds in Disease Treatment
Keywords
equol, phytoestrogens, TNF-α, NF-κB activation, estrogen receptor-independent mechanism, macrophages, osteoprotection, IκBα degradation, p65 nuclear translocation, RAW 264.7 macrophages, LPS-stimulated inflammation, fulvestrant
MeSH terms
Animals, Base Sequence, Cell Line, Enzyme-Linked Immunosorbent Assay, Gene Expression Regulation, Isoflavones, Macrophages, Receptors, Estrogen, Transcription, Genetic, Tumor Necrosis Factor-alpha, NF-kappa B, DNA Primers, Reverse Transcriptase Polymerase Chain Reaction, Protein Transport, Electrophoretic Mobility Shift Assay, Mice, Equol

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