The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
Methods to determine limit of detection and limit of quantification in quantitative real-time PCR (qPCR)
Open accessAbstract
Quantitative Real-Time Polymerase Chain Reaction, better known as qPCR, is the most sensitive and specific technique we have for the detection of nucleic acids. Even though it has been around for more than 30 years and is preferred in research applications, it has yet to win broad acceptance in routine practice. This requires a means to unambiguously assess the performance of specific qPCR analyses. Here we present methods to determine the limit of detection (LoD) and the limit of quantification (LoQ) as applicable to qPCR. These are based on standard statistical methods as recommended by regulatory bodies adapted to qPCR and complemented with a novel approach to estimate the precision of LoD.
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Cites
The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments
Bustin, S. A., Benes, V., Garson, J. A., Hellemans, J., Huggett, J., Kubista, M., Mueller, R., Nolan, T., Pfaffl, M. W., Shipley, G. L., et al. (2009) · Clinical Chemistry · doi:10.1373/clinchem.2008.112797 · Cited by 16,303 · cited 2×
“This non-linear system of equations is solved by GenEx 6 [11], using a quasi-Newton method.”
BackgroundMethodSelf-citationLog-normal distributions across the sciences: keys and clues
LIMPERT, E., STAHEL, W. A., & ABBT, M. (2001) · BioScience · doi:10.1641/0006-3568(2001)051[0341:lndats]2.0.co;2 · Cited by 2,621
The real-time polymerase chain reaction
Kubista, M., Andrade, J. M., Bengtsson, M., Forootan, A., Jonák, J., Lind, K., Sindelka, R., Sjöback, R., Sjögreen, B., Strömbom, L., et al. (2006) · Molecular Aspects of Medicine · doi:10.1016/j.mam.2005.12.007 · Cited by 1,515
Self-citationModelling the limit of detection in real-time quantitative PCR
Burns, M., & Valdivia, H. (2008) · European Food Research and Technology · doi:10.1007/s00217-007-0683-z · Cited by 181
Bias and Imprecision in Analysis of Real-Time Quantitative Polymerase Chain Reaction Data
Tellinghuisen, J., & Spiess, A.-N. (2015) · Analytical Chemistry · doi:10.1021/acs.analchem.5b02057 · Cited by 35
Cited by
Bustin, S., & Huggett, J. (2017) · Biomolecular Detection and Quantification · doi:10.1016/j.bdq.2017.11.001 · Cited by 312
Digital PCR: A Sensitive and Precise Method for KIT D816V Quantification in Mastocytosis
Greiner, G., Gurbisz, M., Ratzinger, F., Witzeneder, N., Simonitsch-Klupp, I., Mitterbauer-Hohendanner, G., Mayerhofer, M., Müllauer, L., Sperr, W. R., Valent, P., et al. (2018) · Clinical Chemistry · doi:10.1373/clinchem.2017.277897 · Cited by 83
Metadata
- Authors
- Forootan, A., Sjöback, R., Björkman, J., Sjögreen, B., Linz, L., & Kubista, M.
- Venue
- Biomolecular Detection and Quantification
- Publisher
- Elsevier BV
- Published
- 2017-06
- Volume
- 12
- Pages
- 1-6
- ISSN
- 2214-7535 (ISSN-L)
- OpenAlex
- W2608363029
- Cited by
- 578
- References
- 5
- FWCI
- 12.91
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Forootan, A., Sjöback, R., Björkman, J., Sjögreen, B., Linz, L., & Kubista, M. (2017). Methods to determine limit of detection and limit of quantification in quantitative real-time PCR (qPCR). Biomolecular Detection and Quantification. https://doi.org/10.1016/j.bdq.2017.04.001
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