3D printing a biocompatible elastomer for modeling muscle regeneration after volumetric muscle loss
Open accessAbstract
Volumetric muscle loss (VML) injuries due to trauma, tumor ablation, or other degenerative muscle diseases are debilitating and currently have limited options for self-repair. Advancements in 3D printing allow for the rapid fabrication of biocompatible scaffolds with designer patterns. However, the materials chosen are often stiff or brittle, which is not optimal for muscle tissue engineering. This study utilized a photopolymerizable biocompatible elastomer - poly (glycerol sebacate) acrylate (PGSA) - to develop an in vitro model of muscle regeneration and proliferation into an acellular scaffold after VML injury. Mechanical properties of the scaffold were tuned by controlling light intensity during the 3D printing process to match the specific tension of skeletal muscle. The effect of both geometric (channel sizes between 300 and 600 μm) and biologic (decellularized muscle extracellular matrix (dECM)) cues on muscle progenitor cell infiltration, proliferation, organization, and maturation was evaluated in vitro using a near-infrared fluorescent protein (iRFP) transfected cell line to assess cells in the 3D scaffold. Larger channel sizes and dECM coating were found to enhance cell proliferation and maturation, while no discernable effect on cell alignment was observed. In addition, a pilot experiment was carried out to evaluate the regenerative capacity of this scaffold in vivo after a VML injury. Overall, this platform demonstrates a simple model to study muscle progenitor recruitment and differentiation into acellular scaffolds after VML repair.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
3D bioprinting enables fabrication of complex muscle tissue constructs with precise control over architecture and composition.
“Recent progress in 3D bioprinting holds significant promise for addressing VML by enabling the fabrication of complex muscle tissue constructs with precise control over architecture and composition [8, 9].”
From Development of silk microfiber-reinforced bioink for muscle tissue engineering and in situ printing by a handheld 3D printer (2025) · cites this paper as [9]
The Introduction and Results section on 3D printing PGSA microchannel scaffolds describe fabrication of VML-oriented scaffolds with intricate architecture and controlled channel sizes. The Methods also describe choosing a PGSA bioink and adding a dECM coating, but the work prints acellular scaffolds rather than complex muscle tissue constructs, and it does not demonstrate precise control of tissue composition through printing. (LLM, conf=0.85)
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Matrix elasticity directs stem cell lineage specification
Engler, A. J., Sen, S., Sweeney, H. L., & Discher, D. E. (2006) · Cell · doi:10.1016/j.cell.2006.06.044 · Cited by 13,915 · see citing works
Geometric Control of Cell Life and Death
Chen, C. S., Mrksich, M., Huang, S., Whitesides, G. M., & Ingber, D. E. (1997) · Science · doi:10.1126/science.276.5317.1425 · Cited by 4,628 · see citing works
The extracellular matrix at a glance
Frantz, C., Stewart, K. M., & Weaver, V. M. (2010) · Journal of Cell Science · doi:10.1242/jcs.023820 · Cited by 4,334 · see citing works
A 3D bioprinting system to produce human-scale tissue constructs with structural integrity
Kang, H.-W., Lee, S. J., Ko, I. K., Kengla, C., Yoo, J. J., & Atala, A. (2016) · Nature Biotechnology · doi:10.1038/nbt.3413 · Cited by 2,622 · see citing works
Continuous liquid interface production of 3D objects
Tumbleston, J. R., Shirvanyants, D. G., Ermoshkin, N., Janusziewicz, R., Johnson, A. R., Kelly, D., Chen, K., PINSCHMIDT, R. K. J., Rolland, J. P., Ermoshkin, A. V., et al. (2015) · Science · doi:10.1126/science.aaa2397 · Cited by 2,295 · see citing works
Dynamic properties of mammalian skeletal muscles.
Close, R. I. (1972) · Physiological Reviews · doi:10.1152/physrev.1972.52.1.129 · Cited by 1,890 · see citing works
Reference Module in Materials Science and Materials Engineering
Macovez, R., & Rudolf, P. (2016) · Elsevier eBooks · Cited by 1,882 · see citing works
A tough biodegradable elastomer
Wang, Y., Ameer, G. A., Sheppard, B. J., & Langer, R. (2002) · Nature Biotechnology · doi:10.1038/nbt0602-602 · Cited by 1,314 · cited 3× · see citing works
“The traditional approach to fabricate PGSA scaffolds requires harsh processing conditions (curing at high temperature and low pressure) that make it difficult to precision engineer structures with fine features and material properties [43].”
BackgroundMethodInfluentialCombat Wounds in Operation Iraqi Freedom and Operation Enduring Freedom
Owens, B. D., Kragh, J. F., Wenke, J. C., Macaitis, J. M., Wade, C. E., & Holcomb, J. B. (2008) · The Journal of Trauma: Injury, Infection, and Critical Care · doi:10.1097/ta.0b013e318163b875 · Cited by 1,106 · see citing works
A Comparative Review of Natural and Synthetic Biopolymer Composite Scaffolds
Reddy, M. S. B., Ponnamma, D., Choudhary, R., & Sadasivuni, K. K. (2021) · Polymers · doi:10.3390/polym13071105 · Cited by 1,104 · see citing works
Overview of natural hydrogels for regenerative medicine applications
Catoira, M. C., Fusaro, L., Francesco, D. D., Ramella, M., & Boccafoschi, F. (2019) · Journal of Materials Science Materials in Medicine · doi:10.1007/s10856-019-6318-7 · Cited by 800 · see citing works
Kinetic Study and New Applications of UV Radiation Curing
Decker, C. (2002) · Macromolecular Rapid Communications · doi:10.1002/marc.200290014 · Cited by 689 · see citing works
Bright and stable near-infrared fluorescent protein for in vivo imaging
Filonov, G. S., Piatkevich, K. D., Ting, L.-M., Zhang, J., Kim, K., & Verkhusha, V. V. (2011) · Nature Biotechnology · doi:10.1038/nbt.1918 · Cited by 682 · see citing works
Engineering microscale topographies to control the cell–substrate interface
Nikkhah, M., Edalat, F., Manoucheri, S., & Khademhosseini, A. (2012) · Biomaterials · doi:10.1016/j.biomaterials.2012.03.079 · Cited by 640 · see citing works
Direct 3D bioprinting of prevascularized tissue constructs with complex microarchitecture
Zhu, W. Z., Qu, X., Zhu, J., Ma, X., Patel, S. H., Liu, J., Wang, P., Lai, C. S. E., Gou, M., Xu, Y., et al. (2017) · Biomaterials · doi:10.1016/j.biomaterials.2017.01.042 · Cited by 568 · see citing works
Self-citationPredictability of skeletal muscle tension from architectural determinations in guinea pig hindlimbs
Powell, P. L., Roy, R. R., Kanim, P., Bello, M. A., & Edgerton, V. R. (1984) · Journal of Applied Physiology · doi:10.1152/jappl.1984.57.6.1715 · Cited by 553 · see citing works
Chen, Q.-Z., Bismarck, A., Hansen, U., Junaid, S., Tran, M. Q., Harding, S. E., Ali, N. N., & Boccaccini, A. R. (2007) · Biomaterials · doi:10.1016/j.biomaterials.2007.09.010 · Cited by 531 · see citing works
Current Status of Bioinks for Micro-Extrusion-Based 3D Bioprinting
Panwar, A., & Tan, L. P. (2016) · Molecules · doi:10.3390/molecules21060685 · Cited by 478 · see citing works
Grogan, B. F., & Hsu, J. R. (2011) · American Academy of Orthopaedic Surgeon · doi:10.5435/00124635-201102001-00007 · Cited by 402 · see citing works
Skeletal muscle design to meet functional demands
Lieber, R. L., & Ward, S. R. (2011) · Philosophical Transactions of the Royal Society B: Biological Sciences · doi:10.1098/rstb.2010.0316 · Cited by 377 · see citing works
Self-citationHemocompatibility evaluation of poly(glycerol-sebacate) in vitro for vascular tissue engineering
Motlagh, D., Yang, J. Y., Lui, K. Y., Webb, A. R., & Ameer, G. A. (2006) · Biomaterials · doi:10.1016/j.biomaterials.2006.04.010 · Cited by 375 · see citing works
Zhang, A. P., Qu, X., Soman, P., Hribar, K. C., Lee, J. W., Chen, S., & He, S. (2012) · Advanced Materials · doi:10.1002/adma.201202024 · Cited by 360 · see citing works
Self-citationSynthesis and Characterization of Photocurable Elastomers from Poly(glycerol- co -sebacate)
Nijst, C. L. E., Bruggeman, J. P., Karp, J. M., Ferreira, L., Zumbuehl, A., Bettinger, C. J., & Langer, R. (2007) · Biomacromolecules · doi:10.1021/bm070423u · Cited by 295 · see citing works
Zhang, Y., He, Y., Bharadwaj, S., Hammam, N., Carnagey, K., Myers, R., Atala, A., & Van Dyke, M. (2009) · Biomaterials · doi:10.1016/j.biomaterials.2009.04.005 · Cited by 276 · see citing works
Skeletal Muscle Structure, Function, and Plasticity
Lieber, R. L. (2009) · Cited by 227 · see citing works
Dziki, J., Badylak, S., Yabroudi, M., Sicari, B., Ambrosio, F., Stearns, K., Turner, N., Wyse, A., Boninger, M. L., Brown, E. H. P., et al. (2016) · npj Regenerative Medicine · doi:10.1038/npjregenmed.2016.8 · Cited by 219 · see citing works
Structural properties of scaffolds: Crucial parameters towards stem cells differentiation
Ghasemi‐Mobarakeh, L. (2015) · World Journal of Stem Cells · doi:10.4252/wjsc.v7.i4.728 · Cited by 214 · see citing works
In vivo specific tension of human skeletal muscle
Maganaris, C. N., Baltzopoulos, V., Ball, D., & Sargeant, A. J. (2001) · Journal of Applied Physiology · doi:10.1152/jappl.2001.90.3.865 · Cited by 192 · see citing works
Eckardt, A. M., & Fokas, K. (2003) · Journal of Cranio-Maxillofacial Surgery · doi:10.1016/s1010-5182(03)00039-8 · Cited by 187 · see citing works
Efficient myotube formation in 3D bioprinted tissue construct by biochemical and topographical cues
Kim, W., Lee, H., Lee, J., Atala, A., Yoo, J. J., Lee, S. J., & Kim, G. H. (2020) · Biomaterials · doi:10.1016/j.biomaterials.2019.119632 · Cited by 185 · see citing works
Role of Mechanical Factors in Fate Decisions of Stem Cells
Li, D., Zhou, J., Chowdhury, F., Cheng, J., Wang, N., & Wang, F. (2011) · Regenerative Medicine · doi:10.2217/rme.11.2 · Cited by 181 · see citing works
Chen, Q., Ishii, H., Thouas, G. A., Lyon, A. R., Wright, J. S., Blaker, J. J., Chrzanowski, W., Boccaccini, A. R., Ali, N. N., Knowles, J. C., et al. (2010) · Biomaterials · doi:10.1016/j.biomaterials.2010.01.108 · Cited by 174 · see citing works
Frog semitendinosis tendon load-strain and stress-strain properties during passive loading
Lieber, R. L., Leonard, M. E., Brown, C. G., & Trestik, C. L. (1991) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.1991.261.1.c86 · Cited by 169 · see citing works
Nerve guides manufactured from photocurable polymers to aid peripheral nerve repair
Pateman, C. J., Harding, A., Glen, A., Taylor, C. S., Christmas, C. R., Robinson, P. P., Rimmer, S., Boissonade, F. M., Claeyssens, F., & Haycock, J. W. (2015) · Biomaterials · doi:10.1016/j.biomaterials.2015.01.055 · Cited by 164 · see citing works
Biological Products: Cellular Therapy and FDA Approved Products
Golchin, A., & Farahany, T. Z. (2019) · Stem Cell Reviews and Reports · doi:10.1007/s12015-018-9866-1 · Cited by 148 · see citing works
Ogrodnik, M. B., Salmonowicz, H., Brown, R., Turkowska, J., Średniawa, W., Pattabiraman, S., Amen, T., Abraham, A.-C., Eichler, N., Lyakhovetsky, R., et al. (2014) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1324035111 · Cited by 143 · see citing works
Zhang, J., Hu, Z. Q., Turner, N. J., Teng, S. F., Cheng, W. Y., Zhou, H. Y., Zhang, L., Hu, H. W., Wang, Q., & Badylak, S. F. (2016) · Biomaterials · doi:10.1016/j.biomaterials.2016.02.040 · Cited by 141 · see citing works
Merritt, E., Hammers, D. W., Tierney, M. T., Suggs, L. J., Walters, J., & Farrar, R. P. (2009) · Tissue Engineering Part A · doi:10.1089/ten.tea.2009.0226 · Cited by 139 · see citing works
Engineering Aligned Skeletal Muscle Tissue Using Decellularized Plant-Derived Scaffolds
Cheng, Y.-W., Shiwarski, D. J., Ball, R. L., Whitehead, K. A., & Feinberg, A. W. (2020) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.0c00058 · Cited by 125 · see citing works
Altomare, L., Gadegaard, N., Visai, L., Tanzi, M. C., & Farè, S. (2009) · Acta Biomaterialia · doi:10.1016/j.actbio.2009.12.040 · Cited by 120 · see citing works
Additive manufactured biodegradable poly(glycerol sebacate methacrylate) nerve guidance conduits
Singh, D., Harding, A. J., Albadawi, E. A., Boissonade, F. M., Haycock, J. W., & Claeyssens, F. (2018) · Acta Biomaterialia · doi:10.1016/j.actbio.2018.07.055 · Cited by 120 · see citing works
Near‐Infrared Molecular Probes for In Vivo Imaging
Zhang, X., Bloch, S. R., Akers, W. J., & Achilefu, S. A. (2012) · Current Protocols in Cytometry · doi:10.1002/0471142956.cy1227s60 · Cited by 116 · see citing works
Tamayol, A., Hassani Najafabadi, A., Mostafalu, P., Yetisen, A. K., Commotto, M., Aldhahri, M., Abdel-wahab, M. S., Najafabadi, Z. I., Latifi, S., Akbari, M., et al. (2017) · Scientific Reports · doi:10.1038/s41598-017-04749-8 · Cited by 115 · see citing works
Skeletal muscle regeneration with robotic actuation–mediated clearance of neutrophils
Seo, B. R., Payne, C. J., McNamara, S. L., Freedman, B. R., Kwee, B. J., Nam, S., de Lázaro, I., Darnell, M., Alvarez, J. T., Dellacherie, M. O., et al. (2021) · Science Translational Medicine · doi:10.1126/scitranslmed.abe8868 · Cited by 111 · see citing works
A Highly Tunable Biocompatible and Multifunctional Biodegradable Elastomer
Pereira, M. J. N., Ouyang, B., Sundback, C. A., Lang, N., Friehs, I., Mureli, S., Pomerantseva, I., McFadden, J. D., Mochel, M. C., Mwizerwa, O., et al. (2012) · Advanced Materials · doi:10.1002/adma.201203824 · Cited by 111 · see citing works
Garg, K., Ward, C., Rathbone, C., & Corona, B. T. (2014) · Cell and Tissue Research · doi:10.1007/s00441-014-2006-6 · Cited by 109 · see citing works
Quantitative analysis of neonatal skeletal muscle functional improvement in the mouse
Gokhin, D. S., Ward, S. R., Bremner, S. N., & Lieber, R. L. (2008) · Journal of Experimental Biology · doi:10.1242/jeb.014340 · Cited by 108 · see citing works
Self-citationEngineering Muscle Constructs for the Creation of Functional Engineered Musculoskeletal Tissue
Mertens, J. P., Sugg, K. B., Lee, J. D., & Larkin, L. M. (2013) · Regenerative Medicine · doi:10.2217/rme.13.81 · Cited by 107 · see citing works
Whence Directionality: Guidance Mechanisms in Solitary and Collective Cell Migration
Rørth, P. (2011) · Developmental Cell · doi:10.1016/j.devcel.2010.12.014 · Cited by 106 · see citing works
Biomimetic poly(glycerol sebacate) (PGS) membranes for cardiac patch application
Rai, R., Tallawi, M., Barbani, N., Frati, C., Madeddu, D., Cavalli, S., Graiani, G., Quaini, F., Roether, J. A., Schubert, D. W., et al. (2013) · Materials Science and Engineering C · doi:10.1016/j.msec.2013.04.058 · Cited by 103 · see citing works
In Vivo Printing of Nanoenabled Scaffolds for the Treatment of Skeletal Muscle Injuries
Quint, J. P., Mostafavi, A., Endo, Y., Panayi, A. C., Russell, C. S., Nourmahnad, A., Wiseman, C., Abbasi, L., Samandari, M., Sheikhi, A., et al. (2021) · Advanced Healthcare Materials · doi:10.1002/adhm.202002152 · Cited by 102 · see citing works
Jin, Y., Shahriari, D., Jeon, E. J., Park, S., Choi, Y. S., Back, J., Lee, H., Anikeeva, P., & Cho, S. (2021) · Advanced Materials · doi:10.1002/adma.202007946 · Cited by 92 · see citing works
Sicari, B. M., Dearth, C. L., & Badylak, S. F. (2014) · The Anatomical Record · doi:10.1002/ar.22794 · Cited by 90 · see citing works
Determination of a Critical Size Threshold for Volumetric Muscle Loss in the Mouse Quadriceps
Anderson, S. E., Han, W. M., Srinivasa, V., Mohiuddin, M., Ruehle, M. A., Moon, J. Y., Shin, E.-J., Emeterio, C. L. S., Ogle, M. E., Botchwey, E. A., et al. (2019) · Tissue Engineering Part C Methods · doi:10.1089/ten.tec.2018.0324 · Cited by 90 · see citing works
Continuous Optical 3D Printing of Green Aliphatic Polyurethanes
Pyo, S., Wang, P., Hwang, H. H., Zhu, W. Z., Warner, J., & Chen, S. (2016) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.6b12500 · Cited by 86 · see citing works
Self-citationZhang, Y., Zhang, Z., Wang, Y., Su, Y., & Chen, M. (2020) · Materials Science and Engineering C · doi:10.1016/j.msec.2020.111070 · Cited by 83 · see citing works
Das, S., Browne, K. D., Laimo, F. A., Maggiore, J. C., Hilman, M. C., Kaisaier, H., Aguilar, C. A., Ali, Z. S., Mourkioti, F., & Cullen, D. K. (2020) · Communications Biology · doi:10.1038/s42003-020-1056-4 · Cited by 81 · see citing works
Marsano, A., Maidhof, R., Wan, L. Q., Wang, Y., Gao, J., Tandon, N. M., & Vunjak‐Novakovic, G. (2010) · Biotechnology Progress · doi:10.1002/btpr.435 · Cited by 77 · see citing works
Nakayama, K. H., Alcazar, C. A., Yang, G., Quarta, M., Paine, P., Doan, L., Davies, A., Rando, T., & Huang, N. F. (2018) · npj Regenerative Medicine · doi:10.1038/s41536-018-0054-3 · Cited by 75 · see citing works
Combined Technologies for Microfabricating Elastomeric Cardiac Tissue Engineering Scaffolds
Guillemette, M. D., Park, H., Hsiao, J. C., Jain, S. R., Larson, B. L., Langer, R., & Freed, L. E. (2010) · Macromolecular Bioscience · doi:10.1002/mabi.201000165 · Cited by 73 · see citing works
Rapid release of growth factors regenerates force output in volumetric muscle loss injuries
Grasman, J. M., Duc, M., Page, R. L., & Pins, G. D. (2015) · Biomaterials · doi:10.1016/j.biomaterials.2015.08.047 · Cited by 73 · see citing works
Aligned and electrically conductive 3D collagen scaffolds for skeletal muscle tissue engineering
Basurto, I. M., Mora, M. T., Gardner, G. M., Christ, G. J., & Caliari, S. R. (2021) · Biomaterials Science · doi:10.1039/d1bm00147g · Cited by 70 · see citing works
Kasukonis, B. M., Kim, J., Brown, L. A., Jones, J. D., Ahmadi, S., Washington, T. A., & Wolchok, J. C. (2016) · Tissue Engineering Part A · doi:10.1089/ten.tea.2016.0134 · Cited by 67 · see citing works
3D bioprinting of hydrogels for retina cell culturing
Wang, P., Li, X., Zhu, W. Z., Zhong, Z., Moran, A., Wang, W., Zhang, K., & Chen, S. (2018) · Bioprinting · doi:10.1016/j.bprint.2018.e00029 · Cited by 66 · see citing works
Self-citationNatural Polymeric Biomaterials: Processing and Properties ☆
dos Santos, L. A. (2017) · Elsevier eBooks · doi:10.1016/b978-0-12-803581-8.02253-0 · Cited by 64 · see citing works
Gilbert‐Honick, J., Iyer, S. R., Somers, S. M., Takasuka, H., Lovering, R. M., Wagner, K. R., Mao, H.-Q., & Grayson, W. L. (2020) · Biomaterials · doi:10.1016/j.biomaterials.2020.120154 · Cited by 62 · see citing works
Carleton, M. M., Locke, M., & Sefton, M. V. (2021) · Biomaterials · doi:10.1016/j.biomaterials.2021.120909 · Cited by 59 · see citing works
Gao, H., Xiao, J., Wei, Y., Wang, H., Wan, H., & Liu, S. (2021) · ACS Omega · doi:10.1021/acsomega.1c02347 · Cited by 54 · see citing works
Wang, P., Berry, D. B., Song, Z., Kiratitanaporn, W., Schimelman, J. B., Moran, A., He, F., Xi, B., Cai, S., & Chen, S. (2020) · Advanced Functional Materials · doi:10.1002/adfm.201910391 · Cited by 52 · see citing works
Self-citationBiofabricating murine and human myo‐substitutes for rapid volumetric muscle loss restoration
Costantini, M., Testa, S., Fornetti, E., Fuoco, C., Riera, C. S., Nie, M., Bernardini, S., Rainer, A., Baldi, J., Zoccali, C., et al. (2021) · EMBO Molecular Medicine · doi:10.15252/emmm.202012778 · Cited by 51 · see citing works
Long-Term Evaluation of Functional Outcomes Following Rat Volumetric Muscle Loss Injury and Repair
Mintz, E. L., Passipieri, J. A., Franklin, I. R., Toscano, V. M., Afferton, E. C., Sharma, P. R., & Christ, G. J. (2019) · Tissue Engineering Part A · doi:10.1089/ten.tea.2019.0126 · Cited by 51 · see citing works
Wu, Y., D’Amato, A. R., Yan, A. M., Wang, R. Q., Ding, X., & Wang, Y. (2020) · ACS Applied Bio Materials · doi:10.1021/acsabm.0c00804 · Cited by 45 · see citing works
Reconstruction of Muscle Fascicle‐Like Tissues by Anisotropic 3D Patterning
Jin, Y., Jeon, E. J., Jeong, S., Min, S., Choi, Y. S., Ys, K., Lee, J. S., Shin, J., Yu, J. H., Ahn, D., et al. (2021) · Advanced Functional Materials · doi:10.1002/adfm.202006227 · Cited by 41 · see citing works
Teng, C.-L., Chen, J., Chang, T.-L., Hsiao, S.-K., Hsieh, Y., Gorday, K. A. V., Cheng, Y., & Wang, J. (2020) · Biofabrication · doi:10.1088/1758-5090/ab69da · Cited by 35 · see citing works
Hernandez, M. J., Zelus, E. I., Spang, M. T., Braden, R. L., & Christman, K. L. (2020) · Biomaterials Science · doi:10.1039/c9bm01963d · Cited by 32 · see citing works
Duong, V. T., Dang, T. T. P., Hwang, C. H., Back, S. H., & Koo, K.-I. (2020) · Biofabrication · doi:10.1088/1758-5090/abafc6 · Cited by 31 · see citing works
Lau, C. C., Al-Qaysi, M., Owji, N., Bayazit, M. K., Xie, J., Knowles, J. C., & Tang, J. (2019) · Materials Today Advances · doi:10.1016/j.mtadv.2019.100023 · Cited by 20 · see citing works
Basturkmen, B., Ergene, E., Doğanay, D., Huri, P. Y., Ünalan, H. E., & Aksoy, E. A. (2021) · Biomaterials Advances · doi:10.1016/j.msec.2021.112567 · Cited by 17 · see citing works
Hall, C., von Grabowiecki, Y., Pearce, S. P., Dive, C., Bagley, S., & Muller, P. A. J. (2021) · Cancer Cell International · doi:10.1186/s12935-021-01918-8 · Cited by 16 · see citing works
Bour, R. K., Sharma, P. R., Turner, J. S., Hess, W. E., Mintz, E. L., Latvis, C., Shepherd, B. R., Presnell, S. C., McConnell, M., Highley, C. B., et al. (2020) · Connective Tissue Research · doi:10.1080/03008207.2019.1679800 · Cited by 14 · see citing works
Flagged first, then by citations
5 of the 85 references couldn’t be matched to a known paper.
Research works printed but not linked: 14
- [26]Gilbert-Honick J, Iyer SR, Somers SM, Takasuka H, Lovering RM, Wagner KR, Engineering 3D skeletal muscle primed for neuromuscular regeneration following volumetric muscle loss. Biomaterials. 2020; 255: 120154. 32562942 PMC11192434
- [27]Kim WJ, Lee H, Lee JU, Atala A, Yoo JJ, Lee SJ, Efficient myotube formation in 3D bioprinted tissue construct by biochemical and topographical cues. Biomaterials. 2020; 230 July 2019: 119632. 31761486 PMC7141931
- [28]Zhang Y, Zhang Z, Wang Y, Su Y, Chen M. 3D myotube guidance on hierarchically organized anisotropic and conductive fibers for skeletal muscle tissue engineering. Mater Sci Eng C. 2020; 116 March: 111070. 32806237
- [30]Bour RK, Sharma PR, Turner JS, Hess WE, Mintz EL, Latvis CR, Bioprinting on sheet-based scaffolds applied to the creation of implantable tissue-engineered constructs with potentially diverse clinical applications: Tissue-Engineered Muscle Repair (TEMR) as a representative testbed. Connect Tissue Res. 2020; 61: 216– 28. 31899969
- [31]Basurto IM, Mora MT, Gardner GM, Christ GJ, Caliari SR. Aligned and electrically conductive 3D collagen scaffolds for skeletal muscle tissue engineering. Biomater Sci. 2021; 9: 4040– 53. 33899845
- [32]Grasman JM, Do DM, Page RL, Pins GD. Rapid release of growth factors regenerates force output in volumetric muscle loss injuries. Biomaterials. 2015; 72: 49– 60. 26344363 PMC4591244
- [33]Zhang J, Hu ZQ, Turner NJ, Teng SF, Cheng WY, Zhou HY, Perfusion-decellularized skeletal muscle as a three-dimensional scaffold with a vascular network template. Biomaterials. 2016; 89: 114– 26. 26963901
- [34]Nakayama KH, Alcazar C, Yang G, Quarta M, Paine P, Doan L, Rehabilitative exercise and spatially patterned nanofibrillar scaffolds enhance vascularization and innervation following volumetric muscle loss. npj Regen Med. 2018; 3. 30245849 PMC6141593
- [35]Das S, Browne KD, Laimo FA, Maggiore JC, Hilman MC, Kaisaier H, Pre-innervated tissue-engineered muscle promotes a pro-regenerative microenvironment following volumetric muscle loss. Commun Biol. 2020; 3: 1– 14. 32587337 PMC7316777
- [68]Nijst CLE, Bruggeman JP, Karp JM, Ferreira L, Zumbuehl A, Bettinger CJ, Synthesis and characterization of photocurable elastomers from poly(glycerol-co-sebacate). Biomacromolecules. 2007; 8: 3067– 73. 17725319 PMC2662850
- [76]Chen Q-Z, Ishii H, Thouas GA, Lyon AR, Wright JS, Blaker JJ, An elastomeric patch derived from poly(glycerol sebacate) for delivery of embryonic stem cells to the heart. Biomaterials. 2010; 31: 3885– 93. 20153041
- [77]Chen Q-Z, Bismarck A, Hansen U, Junaid S, Tran MQ, Harding SE, Characterisation of a soft elastomer poly(glycerol sebacate) designed to match the mechanical properties of myocardial tissue. Biomaterials. 2008; 29: 47– 57. 17915309
- [78]Rai R, Tallawi M, Barbani N, Frati C, Madeddu D, Cavalli S, Biomimetic poly(glycerol sebacate) (PGS) membranes for cardiac patch application. Mater Sci Eng C. 2013; 33: 3677– 87. 23910264
- [89]Altomare L, Gadegaard N, Visai L, Tanzi MC, Farè S. Biodegradable microgrooved polymeric surfaces obtained by photolithography for skeletal muscle cell orientation and myotube development. Acta Biomater. 2010; 6: 1948– 57. 20040385
Cited by
Sorted by citations
Light-based vat-polymerization bioprinting
Levato, R., Dudaryeva, O. Y., Garciamendez‐Mijares, C. E., Kirkpatrick, B. E., Rizzo, R., Schimelman, J. B., Anseth, K. S., Chen, S., Zenobi‐Wong, M., & Zhang, Y. S. (2023) · Nature Reviews Methods Primers · doi:10.1038/s43586-023-00231-0 · Cited by 182 · see citing works
Self-citation4D printed shape-shifting biomaterials for tissue engineering and regenerative medicine applications
Kalogeropoulou, M., Díaz‐Payno, P. J., Mirzaali, M. J., van Osch, G. J. V. M., Fratila-Apachitei, L. E., & ZADPOOR, A. A. (2024) · Biofabrication · doi:10.1088/1758-5090/ad1e6f · Cited by 89 · see citing works
Yang, M. Y., Chu, L., Zhuang, Y., Cheng, Q., Meng, S., Liu, Z., & Kong, T. (2024) · Advanced Functional Materials · doi:10.1002/adfm.202316456 · Cited by 72 · see citing works
Krishna, D. V., & Sankar, M. R. (2023) · Engineered Regeneration · doi:10.1016/j.engreg.2023.07.002 · Cited by 47 · see citing works
Han, S., Cruz, S. H., Park, S., & Shin, S. R. (2023) · Nano Convergence · doi:10.1186/s40580-023-00398-y · Cited by 32 · see citing works
Photopolymerization in 3D printing of tissue-engineered constructs for regenerative medicine
Generalova, A. N., Demina, P. A., Akasov, R., & Khaydukov, E. V. (2023) · Russian Chemical Reviews · doi:10.57634/rcr5068 · Cited by 27 · see citing works
Machine learning-assisted extrusion-based 3D bioprinting for tissue regeneration applications
Krishna, D. V., & Sankar, M. R. (2023) · Annals of 3D Printed Medicine · doi:10.1016/j.stlm.2023.100132 · Cited by 25 · see citing works
Kiratitanaporn, W., Guan, J., Berry, D. B., Lao, A., & Chen, S. (2023) · Tissue Engineering Part A · doi:10.1089/ten.tea.2023.0193 · Cited by 22 · see citing works
Self-citationBiomaterial-Based Regenerative Strategies for Volumetric Muscle Loss: Challenges and Solutions
Cai, C. W., Grey, J. A., Hubmacher, D., & Han, W. M. (2024) · Advances in wound care · doi:10.1089/wound.2024.0079 · Cited by 18 · see citing works
Kamaraj, M., Rezayof, O., Barer, A., Kim, H., Moghimi, N., Joshi, A., Dokmeci, M. R., Khademhosseini, A., Alambeigi, F., & John, J. V. (2025) · Biomaterials Advances · doi:10.1016/j.bioadv.2024.214057 · Cited by 17 · see citing works
Three-Dimensional Bioprinting in Soft Tissue Engineering for Plastic and Reconstructive Surgery
Bülow, A., Schäfer, B., & Beier, J. P. (2023) · Bioengineering · doi:10.3390/bioengineering10101232 · Cited by 16 · see citing works
3D Printing of a Biomimetic Myotendinous Junction Assisted by Artificial Intelligence
Kiratitanaporn, W., Guan, J., Tang, M., Xiang, Y., Lu, T.-Y., Balayan, A., Lao, A., Berry, D. B., & Chen, S. (2024) · Biomaterials Science · doi:10.1039/d4bm00892h · Cited by 14 · see citing works
Self-citationRecent Advances in Implantable Biomaterials for the Treatment of Volumetric Muscle Loss
Schiltz, L., Grivetti, E., Tanner, G. I., & Qazi, T. H. (2024) · Cells Tissues Organs · doi:10.1159/000536262 · Cited by 9 · see citing works
Engineering strategies for the construction of oriented and functional skeletal muscle tissues
Fan, T., Jia, M., Liu, H., Gao, Z., Huang, W., Li, W., & Gu, Q. (2025) · Biofabrication · doi:10.1088/1758-5090/adbfc2 · Cited by 9 · see citing works
Acellular Scaffolds for Muscle Regeneration: Advances and Challenges
Mroueh, J., Weber, L., Endo, Y., Mroueh, V., & Sinha, I. (2025) · Advances in Wound Care · doi:10.1089/wound.2024.0049 · Cited by 3 · see citing works
Three‐dimensional printing of bioengineered scaffolds to support skeletal muscle regeneration
O’Bryan, C. S., Ulery, B. D., & Segal, S. S. (2025) · The Journal of Physiology · doi:10.1113/jp287326 · Cited by 3 · see citing works
Balayan, A., DeBoutray, M., Molley, T. G., Ruoss, S., Maceda, M., Sevier, A., Robertson, C. M., Ward, S. R., & Engler, A. J. (2024) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.00023.2024 · Cited by 2 · see citing works
Self-citationZeng, X. R., Li, Z., Tan, X., Ren, Z., Gao, J., Meng, Z., Li, D., Fan, J., Mao, M., Guo, B., et al. (2025) · Advanced Materials · doi:10.1002/adma.202512137 · Cited by 1 · see citing works
Progressing Regenerative Medicine: Integrating Bioprinting Platforms for Stem Cell Applications
Rajalekshmi, R., & Agrawal, D. K. (2026) · Stem Cells International · doi:10.1155/sci/3319102 · Cited by 1 · see citing works
Morgan, M., Zhang, B., & Narayan, R. J. (2026) · Advanced Engineering Materials · doi:10.1002/adem.202502238
- Authors
- Kiratitanaporn, W., Berry, D. B., Mudla, A., Fried, T., Lao, A., Yu, C., Hao, N., Ward, S. R., & Chen, S.
- Affiliations
- University of California San Diego (US)
- Corresponding author
- Ward, Samuel R.
- Chen, Shaochen
- ORCID
- Venue
- Biomaterials Advances
- Publisher
- Elsevier BV
- Published
- 2022-11
- Volume
- 142
- Pages
- 213171
- ISSN
- 2772-9508 (ISSN-L)
- PMC
- PMC12045644
- OpenAlex
- W4307297270
- Cited by
- 20
- References
- 85
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 7 · 2023: 7
- FWCI
- 4.30
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- www.elsevier.com/legal/tdmrep-license (tdm)
- www.elsevier.com/open-access/userlicense/1.0/ (am, 360 d)
- doi.org/10.15223/policy-017 (stm-asf)
- doi.org/10.15223/policy-037 (stm-asf)
- doi.org/10.15223/policy-012 (stm-asf)
- doi.org/10.15223/policy-029 (stm-asf)
- doi.org/10.15223/policy-004 (stm-asf)
- Locations
- Biomaterials Advances (acceptedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health (P30 NS047101, R21AR074763, R01AR070830-01, R01GM111458)
- University of California, San Diego
- National Science Foundation (1903933, 2135720)
- Topics
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Keywords
- volumetric muscle loss, muscle regeneration, 3D bioprinting, acellular scaffolds, decellularized extracellular matrix, muscle progenitor cells, cell proliferation, cell maturation, channel geometry, light intensity, mechanical properties, in vitro model, in vivo regeneration
- MeSH terms
- Decanoates, Glycerol, Humans, Muscular Diseases, Polymers, Regeneration, Muscle, Skeletal, Elastomers, Tissue Engineering, Printing, Three-Dimensional
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
