A 3D bioprinting system to produce human-scale tissue constructs with structural integrity
Abstract
A challenge for tissue engineering is producing three-dimensional (3D), vascularized cellular constructs of clinically relevant size, shape and structural integrity. We present an integrated tissue-organ printer (ITOP) that can fabricate stable, human-scale tissue constructs of any shape. Mechanical stability is achieved by printing cell-laden hydrogels together with biodegradable polymers in integrated patterns and anchored on sacrificial hydrogels. The correct shape of the tissue construct is achieved by representing clinical imaging data as a computer model of the anatomical defect and translating the model into a program that controls the motions of the printer nozzles, which dispense cells to discrete locations. The incorporation of microchannels into the tissue constructs facilitates diffusion of nutrients to printed cells, thereby overcoming the diffusion limit of 100-200 μm for cell survival in engineered tissues. We demonstrate capabilities of the ITOP by fabricating mandible and calvarial bone, cartilage and skeletal muscle. Future development of the ITOP is being directed to the production of tissues for human applications and to the building of more complex tissues and solid organs.
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Various biofunctional materials and cell types can be printed simultaneously onto a substrate of cell-compatible biomaterials to build 3D complex constructs with good spatial resolution and reproducibility.
“Various biofunctional materials and cell types can thus be printed simultaneously onto a substrate of cell-compatible biomaterials to build 3D complex constructs with good spatial resolution and reproducibility (Fig. 3a)130,148–151.”
From Tumor-on-a-chip: from bioinspired design to biomedical application (2021) · cites this paper as [150]
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- Authors
- Kang, H.-W., Lee, S. J., Ko, I. K., Kengla, C., Yoo, J. J., & Atala, A.
- Affiliations
- Wake Forest University Health Sciences (US)
- Corresponding author
- Atala, Anthony
- Venue
- Nature Biotechnology
- Published
- 2016-02-15
- Volume
- 34
- Issue
- 3
- Pages
- 312-319
- ISSN
- 0733-222X (ISSN-L)
- 1087-0156
- 1546-1696
- OpenAlex
- W2286771936
- Cited by
- 2,622
- References
- 66
- Citations by year
- 2026: 98 · 2025: 199 · 2024: 211 · 2023: 225 · 2022: 296 · 2021: 313 · 2020: 351 · 2019: 298 · 2018: 280 · 2017: 233 · 2016: 113 · 2012: 1
- FWCI
- 148.25
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Scholarworks@UNIST (Ulsan National Institute of Science and Technology) (submittedVersion)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
- Keywords
- 3D bioprinting, vascularized tissue constructs, cell-laden hydrogels, biodegradable polymers, mechanical stability, computer-aided design, microchannels, nutrient diffusion, mandible, calvarial bone, cartilage, skeletal muscle, tissue engineering
- MeSH terms
- Biocompatible Materials, Humans, Mandible, Polymers, Hydrogels, Tissue Engineering, Tissue Scaffolds, Bioprinting, Printing, Three-Dimensional
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