Development of specific collagen scaffolds to support the osteogenic and chondrogenic differentiation of human bone marrow stromal cells
Abstract
Type I Collagen matrices of defined porosity, incorporating carbonate substituted hydroxyapatite (HA) crystals, were assessed for their ability to support osteo- and chondrogenic differentiation of human bone marrow stromal cells (HBMSCs). Collagen-HA composite scaffolds supported the osteogenic differentiation of HBMSCs both in vitro and in vivo as demonstrated by histological and micro-CT analyses indicating the extensive penetration of alkaline phosphatase expressing cells and new matrix synthesis with localised areas immunologically positive for osteocalcin. In vivo, extensive new osteoid formation of implant origin was observed in the areas of vasculature. Chondrogenic matrix synthesis was evidenced in the peripheral regions of pure collagen systems by an abundance of Sox9 expressing chondrocytes embedded within a proteoglycan and collagen II rich ECM. The introduction of microchannels to the scaffold architecture was seen to enhance chondrogenesis. Tissue specific gene expression and corresponding matrix synthesis indicate that collagen matrices support the growth and differentiation of HBMSCs and suggest the potential of this platform for understanding the ECM cues necessary for osteogenesis and chondrogenesis.
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Composite scaffolds based on collagen and HA enhanced the biological properties of seeded cells, resulting in a higher proliferation and differentiation rate, leading to bone regeneration.
“Another study determined that composite scaffolds based on collagen and HA enhanced the biological properties of seeded cells, which resulted in a higher proliferation and differentiation rate, leading to bone regeneration [63].”
From Resorbable Biomaterials Used for 3D Scaffolds in Tissue Engineering: A Review (2023) · cites this paper as [63]
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Giancotti, F. G., & Ruoslahti, E. (1999) · Science · doi:10.1126/science.285.5430.1028 · Cited by 3,869 · see citing works
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High-strength silk protein scaffolds for bone repair
Mandal, B. B., Grinberg, A., Seok Gil, E., Panilaitis, B., & Kaplan, D. L. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1119474109 · Cited by 401 · see citing works
Xia, Z., Yu, X., Jiang, X., Brody, H. D., Rowe, D. W., & Wei, M. (2013) · Acta Biomaterialia · doi:10.1016/j.actbio.2013.03.038 · Cited by 178 · see citing works
Resorbable Biomaterials Used for 3D Scaffolds in Tissue Engineering: A Review
Vach Agocsova, S., Culenova, M., Birova, I., Omanikova, L., Moncmanova, B., Danisovic, L., Ziaran, S., Bakos, D., & Alexy, P. (2023) · Materials · doi:10.3390/ma16124267 · Cited by 98 · see citing works
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Wang, H.-J., Di, L., Ren, Q.-S., & Wang, J.-Y. (2009) · Materials · doi:10.3390/ma2020613 · Cited by 62 · see citing works
- Authors
- Dawson, J. I., Wahl, D. A., Lanham, S. A., Kanczler, J. M., Czernuszka, J. T., & Oreffo, R. O. C.
- Affiliations
- Southampton General Hospital (GB)
- University of Oxford (GB)
- Corresponding author
- Dawson, Jonathan I.
- Venue
- Biomaterials
- Publisher
- Elsevier BV
- Published
- 2008-07
- Volume
- 29
- Issue
- 21
- Pages
- 3105-3116
- ISSN
- 0142-9612 (ISSN-L)
- OpenAlex
- W2022790300
- Cited by
- 101
- References
- 1
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 1 · 2023: 3 · 2022: 2 · 2021: 6 · 2020: 6 · 2019: 5 · 2018: 3 · 2017: 5 · 2016: 5 · 2015: 5 · 2014: 8 · 2013: 6 · 2012: 11
- FWCI
- 6.91
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biomaterials (publishedVersion)
- PubMed (publishedVersion)
- ePrints Soton (University of Southampton) (submittedVersion)
- Funders
- Biotechnology and Biological Sciences Research Council
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Physical Sciences › Materials Science › Biomaterials › Collagen: Extraction and Characterization
- Keywords
- collagen scaffolds, type I collagen, hydroxyapatite, mesenchymal stem cells, osteogenic differentiation, chondrogenic differentiation, osteoinduction, microchannels, extracellular matrix, SOX9, osteocalcin, alkaline phosphatase, osteoid formation, tissue engineering
- MeSH terms
- Biocompatible Materials, Bone Marrow Cells, Cell Differentiation, Cell Survival, Collagen, Humans, Microscopy, Electron, Scanning, Osteogenesis, Stromal Cells, Chondrogenesis, Tissue Engineering, Tissue Scaffolds
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