Fabrication and characterization of biomimetic collagen–apatite scaffolds with tunable structures for bone tissue engineering
Open accessAbstract
The objective of the current study is to prepare a biomimetic collagen-apatite scaffold for improved bone repair and regeneration. A novel bottom-up approach has been developed, which combines a biomimetic self-assembly method with a controllable freeze-casting technology. In this study, the mineralized collagen fibers were generated using a simple one-step co-precipitation method which involved collagen self-assembly and in situ apatite precipitation in a collagen-containing modified simulated body fluid (m-SBF). The precipitates were then subjected to controllable freeze casting, forming scaffolds with either an isotropic equiaxed structure or a unidirectional lamellar structure. These scaffolds were comprised of collagen fibers and poorly crystalline bone-like carbonated apatite nanoparticles. The mineral content in the scaffold could be tailored in the range 0-54wt.% by simply adjusting the collagen content in the m-SBF. Further, the mechanisms of the formation of both the equiaxed and the lamellar scaffolds were investigated, and freezing regimes for equiaxed and lamellar solidification were established. Finally, the bone-forming capability of such prepared scaffolds was evaluated in vivo in a mouse calvarial defect model. It was confirmed that the scaffolds well support new bone formation.
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Through freeze drying, pore size and shape can be controlled by tuning the freezing rate, freezing temperature, and direction of ice growth.
“Through this technique, the pore size and shape can be controlled by tuning the freezing rate, freezing temperature, and direction of the ice growth [85,86].”
From Nano-hydroxyapatite/collagen composite as scaffold material for bone regeneration. (2023) · cites this paper as [85]
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- Authors
- Xia, Z., Yu, X., Jiang, X., Brody, H. D., Rowe, D. W., & Wei, M.
- Affiliations
- University of Connecticut (US)
- UConn Health (US)
- Corresponding author
- Wei, Mei
- Venue
- Acta Biomaterialia
- Publisher
- Elsevier BV
- Published
- 2013-07
- Volume
- 9
- Issue
- 7
- Pages
- 7308-7319
- ISSN
- 1742-7061 (ISSN-L)
- PMC
- PMC3738228
- OpenAlex
- W1997153598
- Cited by
- 178
- References
- 64
- Citations by year
- 2026: 4 · 2025: 9 · 2024: 12 · 2023: 10 · 2022: 12 · 2021: 14 · 2020: 14 · 2019: 14 · 2018: 15 · 2017: 17 · 2016: 16 · 2015: 20 · 2014: 15 · 2013: 6
- FWCI
- 10.47
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Acta Biomaterialia (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Science Foundation (CBET 1133883, IIP 1243455)
- National Institutes of Health (1R21AR059962-01A1)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Physical Sciences › Materials Science › Biomaterials › Collagen: Extraction and Characterization
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Keywords
- biomimetic self-assembly, freeze casting, co-precipitation, bone regeneration, calvarial defect, bone tissue engineering
- MeSH terms
- Animals, Apatites, Bone Development, Bone Regeneration, Collagen, Equipment Design, Materials Testing, Mice, Transgenic, Skull Fractures, Treatment Outcome, Bone Substitutes, Equipment Failure Analysis, Tissue Engineering, Biomimetic Materials, Mice, Tissue Scaffolds
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