On-line observation of cell growth in a three-dimensional matrix on surface-modified microelectrode arrays
Open accessAbstract
Despite many successful applications of microelectrode arrays (MEAs), typical two-dimensional in-vitro cultures do not project the full scale of the cell growth environment in the three-dimensional (3D) in-vivo setting. This study aims to on-line monitor in-vitro cell growth in a 3D matrix on the surface-modified MEAs with a dynamic perfusion culture system. A 3D matrix consisting of poly(ethylene glycol) hydrogel supplemented with poly-D-lysine was subsequently synthesized in situ on the self-assembled monolayer modified MEAs. FTIR spectrum analysis revealed a peak at 2100 cm(-1) due to the degradation of the structure of the 3D matrix. After 2 wks, microscopic examination revealed that the non-degraded area was around 1500 microm(2) and provided enough space for cell growth. Fluorescence microscopy revealed that the degraded 3D matrix was non-cytotoxic allowing the growth of NIH3T3 fibroblasts and cortical neurons in vitro. Time-course changes of total impedance including resistance and reactance were recorded for 8 days to evaluate the cell growth in the 3D matrix on the MEA. A consistent trend reflecting changes of reactance and total impedance was observed. These in-vitro assays demonstrate that our 3D matrix can construct a biomimetic system for cell growth and analysis of cell surface interactions.
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Lin et al. used a flat microelectrode array to monitor impedance change during the growth of NIH 3T3 fibroblasts and cortical neurons in a 3D matrix in a label-free manner.
“Lin et al . used a flat microelectrode array to monitor the impedance change during the growth of NIH 3T3 fibroblasts and cortical neurons in a 3D matrix in a label-free manner. 85”
From Three-dimensional cell culture systems and their applications in drug discovery and cell-based biosensors (2014) · cites this paper as [85]
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Cites
Taking Cell-Matrix Adhesions to the Third Dimension
Cukierman, E., Pankov, R., Stevens, D. R., & Yamada, K. M. (2001) · Science · doi:10.1126/science.1064829 · Cited by 3,027 · see citing works
Capturing complex 3D tissue physiology in vitro
Griffith, L. G., & Swartz, M. A. (2006) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1858 · Cited by 2,274 · see citing works
Three-Dimensional Cell Culture Matrices: State of the Art
Lee, J., Cuddihy, M. J., & Kotov, N. A. (2008) · Tissue Engineering Part B: Reviews · doi:10.1089/teb.2007.0150 · Cited by 1,047 · see citing works
Burdick, J. A., & Anseth, K. S. (2002) · Biomaterials · doi:10.1016/s0142-9612(02)00176-x · Cited by 1,008 · see citing works
Brain responses to micro-machined silicon devices
Szarowski, D. H., Andersen, M. D., Retterer, S. T., Spence, A. J., Isaacson, M. S., Craighead, H. G., Turner, J. N., & Shain, W. G. (2003) · Brain Research · doi:10.1016/s0006-8993(03)03023-3 · Cited by 798 · see citing works
Self-cross-linking biopolymers as injectable in situ forming biodegradable scaffolds
Balakrishnan, B., & Jayakrishnan, A. (2004) · Biomaterials · doi:10.1016/j.biomaterials.2004.10.005 · Cited by 687 · see citing works
Impedance Characterization and Modeling of Electrodes for Biomedical Applications
Franks, W., Schenker, I., Schmutz, P., & Hierlemann, A. R. (2005) · IEEE Transactions on Biomedical Engineering · doi:10.1109/tbme.2005.847523 · Cited by 660 · see citing works
Long-term motor cortex plasticity induced by an electronic neural implant
Andrew, J. O., Mavoori, J., & Fetz, E. E. (2006) · Nature · doi:10.1038/nature05226 · Cited by 454 · see citing works
A novel 3D mammalian cell perfusion-culture system in microfluidic channels
Toh, Y.-C., Zhang, C., Zhang, J., Khong, Y. M., Chang, S., Samper, V. D., van Noort, D., Hutmacher, D. W., & Yu, H. (2007) · Lab on a Chip · doi:10.1039/b614872g · Cited by 414 · see citing works
Computerized microfluidic cell culture using elastomeric channels and Braille displays
Gu, W., Zhu, X. A., Futai, N., Cho, B. S., & Takayama, S. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0404353101 · Cited by 403 · see citing works
Complex impedance spectroscopy for monitoring tissue responses to inserted neural implants
Williams, J. C., Hippensteel, J. A., Dilgen, J., Shain, W. G., & Kipke, D. R. (2007) · Journal of Neural Engineering · doi:10.1088/1741-2560/4/4/007 · Cited by 399 · see citing works
Three-dimensional growth and function of neural tissue in degradable polyethylene glycol hydrogels
Mahoney, M. J., & Anseth, K. S. (2005) · Biomaterials · doi:10.1016/j.biomaterials.2005.11.007 · Cited by 377 · see citing works
Differentiation-on-a-chip: A microfluidic platform for long-term cell culture studies
Tourovskaia, A., Figueroa, X. A., & Folch, A. (2004) · Lab on a Chip · doi:10.1039/b405719h · Cited by 364 · see citing works
Peyton, S. R., Raub, C. B., Keschrumrus, V., & Putnam, A. J. (2006) · Biomaterials · doi:10.1016/j.biomaterials.2006.05.012 · Cited by 357 · see citing works
Impedance Spectroscopy: A Powerful Tool for Rapid Biomolecular Screening and Cell Culture Monitoring
K'Owino, I. O., & Sadik, O. A. (2005) · Electroanalysis · doi:10.1002/elan.200503371 · Cited by 294 · see citing works
Spataro, L. E., Dilgen, J., Retterer, S. T., Spence, A. J., Isaacson, M. S., Turner, J. N., & Shain, W. G. (2005) · Experimental Neurology · doi:10.1016/j.expneurol.2004.08.037 · Cited by 215 · see citing works
Shin, H., Ruhé, P. Q., Mikos, A. G., & Jansen, J. A. (2003) · Biomaterials · doi:10.1016/s0142-9612(03)00168-6 · Cited by 204 · see citing works
Detection of physiologically active compounds using cell-based biosensors
Stenger, D. A., Gross, G. W., Keefer, E. W., Shaffer, K. M., Andreadis, J. D., Ma, W., & Pancrazio, J. J. (2001) · Trends in biotechnology · doi:10.1016/s0167-7799(01)01690-0 · Cited by 196 · see citing works
Shapira‐Schweitzer, K., & Seliktar, D. (2006) · Acta Biomaterialia · doi:10.1016/j.actbio.2006.09.003 · Cited by 147 · see citing works
Chiappalone, M., Vato, A., Tedesco, M., et al. (2003) · Biosensors and Bioelectronics · doi:10.1016/s0956-5663(03)00041-1 · Cited by 128 · see citing works
Newbold, C., Richardson, R. T., Huang, C. Q., Milojevic, D., Cowan, R., & Shepherd, R. K. (2004) · Journal of Neural Engineering · doi:10.1088/1741-2560/1/4/005 · Cited by 113 · see citing works
Cardiomyocyte-transistor-hybrids for sensor application
Ingebrandt, S., Yeung, C. K., Krause, M., & Offenhäusser, A. (2001) · Biosensors and Bioelectronics · doi:10.1016/s0956-5663(01)00170-1 · Cited by 102 · see citing works
Characterization of Three-Dimensional Tissue Cultures Using Electrical Impedance Spectroscopy
Kyle, A. H., Chan, C. T., & Minchinton, A. I. (1999) · Biophysical Journal · doi:10.1016/s0006-3495(99)77416-3 · Cited by 99 · see citing works
Keefer, E. W., Gramowski, A., Stenger, D. A., et al. (2001) · Biosensors and Bioelectronics · doi:10.1016/s0956-5663(01)00165-8 · Cited by 94 · see citing works
Widge, A. S., Jeffries‐EL, M., Cui, X. T., Lagenaur, C. F., & Matsuoka, Y. (2006) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2006.08.011 · Cited by 94 · see citing works
Hydrogel effects on bone marrow stromal cell response to chondrogenic growth factors
Coleman, R. M., Case, N. D., & Guldberg, R. E. (2007) · Biomaterials · doi:10.1016/j.biomaterials.2007.01.010 · Cited by 88 · see citing works
Drug testing on 3D in vitro tissues trapped on a microcavity chip
Kloß, D., Fischer, M., Rothermel, A., Simon, J. C., & Robitzki, A. A. (2008) · Lab on a Chip · doi:10.1039/b800394g · Cited by 86 · see citing works
Wang, L., Zhu, J., Deng, C. L., Xing, W.-L., & Cheng, J. (2008) · Lab on a Chip · doi:10.1039/b804130j · Cited by 85 · see citing works
Impedance Characterization of Microarray Recording Electrodes in Vitro
Merrill, D. R., & Tresco, P. A. (2005) · IEEE Transactions on Biomedical Engineering · doi:10.1109/tbme.2005.856245 · Cited by 74 · see citing works
Lore, A. B., Hubbell, J. A., Bobb, D. S., et al. (1999) · Journal of Neuroscience · doi:10.1523/jneurosci.19-07-02442.1999 · Cited by 62 · see citing works
Liao, J., Lin, S., & Wu, Y.-T. (2004) · Biomacromolecules · doi:10.1021/bm0494951 · Cited by 60 · see citing works
Self-citationA neural probe process enabling variable electrode configurations
Kindlundh, M., Peter Norlin, A., & Hofmann, U. (2004) · Sensors and Actuators B Chemical · doi:10.1016/j.snb.2003.10.009 · Cited by 51 · see citing works
Neuroelectronic interfacing with cultured multielectrode arrays toward a cultured probe
Rutten, W. L. C., Mouveroux, J. M. P., Buitenweg, J. R., Heida, T., Ruardij, T. G., Marani, E., & Lakke, E. A. J. F. (2001) · Proceedings of the IEEE · doi:10.1109/5.939810 · Cited by 46 · see citing works
Electric cell-substrate impedance sensing with screen printed electrode structures
Brischwein, M., Herrmann, S., Vonau, W., et al. (2006) · Lab on a Chip · doi:10.1039/b602987f · Cited by 42 · see citing works
Guertin, D. A., & Sabatini, D. M. (2006) · Encyclopedia of Life Sciences · doi:10.1038/npg.els.0003359 · Cited by 39 · see citing works
Acute neuropharmacologic action of chloroquine on cortical neurons in vitro
O’Shaughnessy, T. J., Zim, B., Ma, W., et al. (2002) · Brain Research · doi:10.1016/s0006-8993(02)03763-0 · Cited by 33 · see citing works
Frampton, J. P., Hynd, M. R., Williams, J. C., Shuler, M. L., & Shain, W. G. (2007) · Journal of Neural Engineering · doi:10.1088/1741-2560/4/4/006 · Cited by 31 · see citing works
Extracellular detection of active membrane currents in the neuron–electrode interface
Buitenweg, J. R., Rutten, W. L. C., Marani, E., Polman, S. K. L., & Ursum, J. (2002) · Journal of Neuroscience Methods · doi:10.1016/s0165-0270(02)00021-3 · Cited by 25 · see citing works
Chou, H. A., Zavitz, D. H., & Ovadia, M. (2002) · Biosensors and Bioelectronics · doi:10.1016/s0956-5663(02)00116-1 · Cited by 20 · see citing works
Characterization of rat spinal cord neurons cultured in defined media on microelectrode arrays
Manos, P., Pancrazio, J. J., Coulombe, M. G., et al. (1999) · Neuroscience Letters · doi:10.1016/s0304-3940(99)00520-0 · Cited by 19 · see citing works
Chiappalone, M., Vato, A., Tedesco, M., et al. (2003) · Cited by 15 · see citing works
Direct Conversion of Bulk Materials into MFI Zeolites by a Bulk-Material Dissolution Technique
Shimizu, S., & Hamada, H. (2000) · Advanced Materials · doi:10.1002/1521-4095(200009)12:18 · Cited by 13 · see citing works
Characterization of surface modification on microelectrode arrays for in vitro cell culture
Lin, S., Chen, J.-J. J., Liao, J., et al. (2007) · Biomedical Microdevices · doi:10.1007/s10544-007-9114-y · Cited by 12 · see citing works
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- Authors
- Lin, S.-P., Kyriakides, T. R., & Chen, J.-J. J.
- Affiliations
- Yale University (US)
- National Cheng Kung University (TW)
- Corresponding author
- Chen, Jia-Jin J.
- Venue
- Biomaterials
- Publisher
- Elsevier BV
- Published
- 2009-06
- Volume
- 30
- Issue
- 17
- Pages
- 3110-3117
- ISSN
- 0142-9612 (ISSN-L)
- PMC
- PMC4658515
- OpenAlex
- W2150914040
- Cited by
- 25
- References
- 43
- Citations by year
- 2023: 1 · 2022: 1 · 2021: 2 · 2020: 2 · 2019: 1 · 2018: 1 · 2017: 3 · 2015: 1 · 2014: 3 · 2013: 3 · 2012: 5
- FWCI
- 0.97
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Biomaterials (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Science Council
- National Institutes of Health (5r01gm072194-11)
- National Institute of Mental Health
- National Institute of General Medical Sciences (R01-GM072194)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neural Engineering
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
- Physical Sciences › Materials Science › Surfaces, Coatings and Films › Polymer Surface Interaction Studies
- Keywords
- microelectrode arrays, 3D cell culture, poly(ethylene glycol) hydrogels, poly-D-lysine, self-assembled monolayers, impedance spectroscopy, cell growth monitoring, hydrogel degradation, NIH3T3 cells, cortical neurons, cell-surface interactions
- MeSH terms
- Animals, Biocompatible Materials, Cells, Cultured, Cerebral Cortex, Equipment Design, Fibroblasts, Fluorescent Dyes, Gold, Indoles, Microelectrodes, Microscopy, Fluorescence, Neurons, Online Systems, Perfusion, Phalloidine, Polyethylene Glycols, Rhodamines, Substrate Specificity, Surface Properties, Time Factors, Electric Impedance, Spectroscopy, Fourier Transform Infrared, Cell Culture Techniques, Hydrogel, Polyethylene Glycol Dimethacrylate, Biomimetics, NIH 3T3 Cells, Cell Proliferation, Mice
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