A novel 3D mammalian cell perfusion-culture system in microfluidic channels
Abstract
Mammalian cells cultured on 2D surfaces in microfluidic channels are increasingly used in drug development and biological research applications. These systems would have more biological or clinical relevance if the cells exhibit 3D phenotypes similar to the cells in vivo. We have developed a microfluidic channel based system that allows cells to be perfusion-cultured in 3D by supporting them with adequate 3D cell-cell and cell-matrix interactions. The maximal cell-cell interaction was achieved by perfusion-seeding cells through an array of micropillars; and 3D cell-matrix interactions were achieved by a polyelectrolyte complex coacervation process to form a thin layer of matrix conforming to the 3D cell shapes. Carcinoma cell lines (HepG2, MCF7), primary differentiated (hepatocytes) and primary progenitor cells (bone marrow mesenchymal stem cells) were perfusion-cultured for 72 hours to 1 week in the microfluidic channel, which preserved their 3D cyto-architecture and cell-specific functions or differentiation competence. This transparent 3D microfluidic channel-based cell culture system also allows direct optical monitoring of cellular events for a wide range of applications.
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To derive more complex functions, organoids with multi-cellular and multi-tissue structures will be important, especially for studying cell-cell interactions.
“To derive more complex functions, organoids with multi-cellular and multi-tissue structures will be important, especially in the context of studying cell-cell interactions246.”
From Organoids (2022) · cites this paper as [246]
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- Authors
- Toh, Y.-C., Zhang, C., Zhang, J., Khong, Y. M., Chang, S., Samper, V. D., van Noort, D., Hutmacher, D. W., & Yu, H.
- Affiliations
- National University of Singapore (SG)
- Institute of Bioengineering and Nanotechnology (SG)
- Corresponding author
- Yu, Hanry
- Venue
- Lab on a Chip
- Publisher
- Royal Society of Chemistry (RSC)
- Published
- 2007
- Volume
- 7
- Issue
- 3
- Pages
- 302
- ISSN
- 1473-0189 (ISSN-L)
- 1473-0197
- OpenAlex
- W2149893376
- Cited by
- 414
- References
- 53
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 13 · 2023: 10 · 2022: 14 · 2021: 11 · 2020: 15 · 2019: 18 · 2018: 28 · 2017: 32 · 2016: 23 · 2015: 21 · 2014: 31 · 2013: 22 · 2012: 29
- FWCI
- 25.10
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Lab on a Chip (publishedVersion)
- PubMed (publishedVersion)
- National University of Singapore (submittedVersion)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Keywords
- 3D cell culture, perfusion culture, microfluidic channels, cell-matrix interactions, cell-cell interactions, hepatocytes, bone marrow mesenchymal stem cells, MCF-7 cells, HepG2 cells
- MeSH terms
- Animals, Bone Marrow Cells, Male, Microscopy, Electron, Scanning, Perfusion, Rats, Wistar, Microscopy, Confocal, Cell Culture Techniques, Hepatocytes, Microfluidics, Rats, Mesenchymal Stem Cells
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