Antibody conjugated magnetic iron oxide nanoparticles for cancer cell separation in fresh whole blood
Open accessAbstract
A highly efficient process using iron oxide magnetic nanoparticles (IO)-based immunomagnetic separation of tumor cells from fresh whole blood has been developed. The process involved polymer coated 30 nm IO that was modified with antibodies (Ab) against human epithelial growth factor receptor 2 (anti-HER2 or anti-HER2/neu) forming IO-Ab. HER2 is a cell membrane protein that is overexpressed in several types of human cancer cells. Using a HER2/neu overexpressing human breast cancer cell line, SK-BR3, as a model cell, the IO-Ab was used to separate 73.6% (with a maximum capture of 84%) of SK-BR3 cells that were spiked in 1 mL of fresh human whole blood. The IO-Ab preferentially bound to SK-BR3 cells over normal cells found in blood due to the high level of HER2/neu receptor on the cancer cells unlike the normal cell surfaces. The results showed that the nanosized magnetic nanoparticles exhibited an enrichment factor (cancer cells over normal cells) of 1:10,000,000 in a magnetic field (with gradient of 100 T/m) through the binding of IO-Ab on the cell surface that resulted in the preferential capture of the cancer cells. This research holds promise for efficient separation of circulating cancer cells in fresh whole blood.
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MACS can accurately detect less than 50 CTC in up to 10 billion total blood cells.
“As an example, less than 50 CTC can be accurately detected in up to 10 billion total blood cells [203].”
From Iron oxide nanoparticles: Diagnostic, therapeutic and theranostic applications (2019) · cites this paper as [203]
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Iron oxide nanoparticles: Diagnostic, therapeutic and theranostic applications
Dadfar, S. M., Roemhild, K., Drude, N. I., von Stillfried, S., Knüchel, R., Kiessling, F., & Lammers, T. (2019) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2019.01.005 · Cited by 1,181 · see citing works
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Multifunctional superparamagnetic iron oxide nanoparticles: Promising tools in cancer theranostics
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- Authors
- Xu, H., Aguilar, Z. P., Yang, L., Kuang, M., Duan, H., Xiong, Y., Wei, H., & Wang, A.
- Affiliations
- Nanchang University (CN)
- Ocean NanoTech (United States) (US)
- Emory University (US)
- Nanyang Technological University (SG)
- Corresponding author
- Wang, Andrew
- Venue
- Biomaterials
- Publisher
- Elsevier BV
- Published
- 2011-12
- Volume
- 32
- Issue
- 36
- Pages
- 9758-9765
- ISSN
- 0142-9612 (ISSN-L)
- PMC
- PMC3220927
- OpenAlex
- W1975359123
- Cited by
- 381
- References
- 2
- Citations by year
- 2026: 4 · 2025: 19 · 2024: 19 · 2023: 19 · 2022: 23 · 2021: 33 · 2020: 31 · 2019: 36 · 2018: 29 · 2017: 30 · 2016: 36 · 2015: 30 · 2014: 19 · 2013: 32 · 2012: 20
- FWCI
- 13.65
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- Locations
- Biomaterials (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institute of Health (R43 CA134066 and 5 R44 RR028019-03)
- National Science Foundation (0810626)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Characterization and Applications of Magnetic Nanoparticles
- Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Bio-sensing Technologies
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Keywords
- immunomagnetic separation, iron oxide nanoparticles, antibody-conjugated nanoparticles, HER2/neu, circulating tumor cells, whole blood, breast cancer, SK-BR-3 cells, magnetic cell capture, enrichment factor, polymer-coated nanoparticles
- MeSH terms
- Antibodies, Blood, Body Fluids, Buffers, Electrophoresis, Polyacrylamide Gel, Female, Ferric Compounds, Humans, Neoplasms, Particle Size, Immunomagnetic Separation, Receptor, ErbB-2, Erb-b2 Receptor Tyrosine Kinases, Cell Line, Tumor, Nanoparticles, Metal Nanoparticles, Magnetic Phenomena
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