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Biologic scaffold composed of skeletal muscle extracellular matrix
Open accessAbstract
Biologic scaffolds prepared from the extracellular matrix (ECM) of decellularized mammalian tissues have been shown to facilitate constructive remodeling in injured tissues such as skeletal muscle, the esophagus, and lower urinary tract, among others. The ECM of every tissue has a unique composition and structure that likely has direct effects on the host response and it is plausible that ECM harvested from a given tissue would provide distinct advantages over ECM harvested from nonhomologous tissues. For example, a tissue specific muscle ECM scaffold may be more suitable for constructive remodeling of skeletal muscle than non-homologous ECM tissue sources. The present study describes an enzymatic and chemical decellularization process for isolating skeletal muscle ECM scaffolds using established decellularization criteria and characterized the structure and chemical composition of the resulting ECM. The results were compared to those from a non-muscle ECM derived from small intestine (SIS). Muscle ECM was shown to contain growth factors, glycosaminoglycans, and basement membrane structural proteins which differed from those present in SIS. Myogenic cells survived and proliferated on muscle ECM scaffolds in vitro, and when implanted in a rat abdominal wall injury model in vivo was shown to induce a constructive remodeling response associated with scaffold degradation and myogenesis in the implant area; however, the remodeling outcome did not differ from that induced by SIS by 35 days post surgery. These results suggest that superior tissue remodeling outcomes are not universally dependent upon homologous tissue derived ECM scaffold materials.
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“Following implantation, these natural ECM scaffolds undergo a degree of degradation, releasing matricryptic peptides and growth factors. This process triggers a shift in macrophage polarization from pro-inflammatory (M1) phenotype to an anti-inflammatory regenerative phenotype (M2 macrophages), promoting angiogenesis and recruiting stem/progenitor cell toward the implanted tissue for constructive”
From Liver tissue engineering using decellularized scaffolds: Current progress, challenges, and opportunities (2024) · cites this paper as [58]
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1 retracted
RETRACTED: Clinical transplantation of a tissue-engineered airway
Macchiarini, P., Jungebluth, P., Go, T., Asnaghi, M. A., Rees, L., Cogan, T. A., Dodson, A., Martorell, J., Bellini, S., Parnigotto, P. P., et al. (2008) · The Lancet · doi:10.1016/s0140-6736(08)61598-6 · Cited by 1,431
RetractedA perivascular origin for mesenchymal stem cells in multiple human organs
Crisan, M., Yap, S., Casteilla, L., Chen, C.-W., Corselli, M., Park, T. S., Andriolo, G., Sun, B., Zheng, B., Zhang, L., et al. (2008) · Cell Stem Cell · doi:10.1016/j.stem.2008.07.003 · Cited by 3,991 · cited 1×
“[38]), and passage 8e12 was utilized for all experiments.”
Self-citationAn overview of tissue and whole organ decellularization processes
Crapo, P. M., Gilbert, T. W., & Badylak, S. F. (2011) · Biomaterials · doi:10.1016/j.biomaterials.2011.01.057 · Cited by 3,591
Self-citationSocializing with the Neighbors
Fuchs, E. V., Tumbar, T., & Guasch, G. (2004) · Cell · doi:10.1016/s0092-8674(04)00255-7 · Cited by 1,737
Asymmetric self-renewal and commitment of satellite stem cells in muscle
Kuang, S., Kuroda, K., Le Grand, F., & Rudnicki, M. A. (2007) · Cell · doi:10.1016/j.cell.2007.03.044 · Cited by 1,449 · cited 1×
“This niche environment is the sum of both structural and biochemical milieu that includes collagens, glycosaminoglycans, proteoglycans, and bound growth factors, among others that may influence myogenesis and the remodeling response [46].”
Uygun, B. E., Soto-Gutierrez, A., Yagi, H., Izamis, M.-L., Guzzardi, M. A., Shulman, C., Milwid, J., Kobayashi, N., Tilles, A., Berthiaume, F., et al. (2010) · Nature Medicine · doi:10.1038/nm.2170 · Cited by 1,370
Tissue-Engineered Lungs for in Vivo Implantation
Petersen, T. H., Calle, E. A., Zhao, L., Lee, E. J., Gui, L., Raredon, M. S. B., Gavrilov, K., Yi, T., Zhuang, Z. W., Breuer, C. K., et al. (2010) · Science · doi:10.1126/science.1189345 · Cited by 1,189
Regeneration and orthotopic transplantation of a bioartificial lung
Ott, H. C., Clippinger, B., Conrad, C., Schuetz, C., Pomerantseva, I., Ikonomou, L., Kotton, D., & Vacanti, J. P. (2010) · Nature Medicine · doi:10.1038/nm.2193 · Cited by 1,090
The extracellular matrix as a biologic scaffold material
Badylak, S. F. (2007) · Biomaterials · doi:10.1016/j.biomaterials.2007.04.043 · Cited by 1,022
Self-citationBrown, B. N., Valentin, J. E., Stewart-Akers, A. M., McCabe, G. P., & Badylak, S. F. (2009) · Biomaterials · doi:10.1016/j.biomaterials.2008.11.040 · Cited by 896
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Yang, Z., Song, Z., Nie, X., Guo, K., & Gu, Y. (2020) · Figshare · doi:10.6084/m9.figshare.13364399
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Metadata
- Authors
- Wolf, M. T., Daly, K. A., Reing, J. E., & Badylak, S. F.
- Affiliations
- University of Pittsburgh (US)
- Corresponding author
- Badylak, Stephen F.
- Venue
- Biomaterials
- Publisher
- Elsevier BV
- Published
- 2012-04
- Volume
- 33
- Issue
- 10
- Pages
- 2916-2925
- ISSN
- 0142-9612 (ISSN-L)
- PMC
- PMC5942557
- OpenAlex
- W2050261148
- Cited by
- 259
- References
- 48
- Citations by year
- 2026: 4 · 2025: 9 · 2024: 16 · 2023: 5 · 2022: 17 · 2021: 20 · 2020: 20 · 2019: 24 · 2018: 19 · 2017: 23 · 2016: 26 · 2015: 25 · 2014: 21 · 2013: 25 · 2012: 4
- FWCI
- 24.49
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Licence
- Locations
- Biomaterials (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- University of Pittsburgh
- National Institutes of Health
- National Heart, Lung, and Blood Institute (T32-HL76124-6, T32HL076124)
- Topics
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Physical Sciences › Materials Science › Biomaterials › Electrospun Nanofibers in Biomedical Applications
- Keywords
- decellularization, biological scaffolds, small intestinal submucosa, tissue remodeling, myogenesis, abdominal wall injury, scaffold degradation, growth factors, glycosaminoglycans, basement membrane proteins
- MeSH terms
- Animals, DNA, Dogs, Extracellular Matrix, Glycosaminoglycans, Humans, Materials Testing, Oxazines, Staining and Labeling, Xanthenes, Reproducibility of Results, Muscle, Skeletal, Prosthesis Implantation, Sus scrofa, Abdominal Wall, Intercellular Signaling Peptides and Proteins, NIH 3T3 Cells, Mice, Rats, Tissue Scaffolds
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