The DOI matches its Crossref record; 44 of the 45 references it cites were found in a registry or an index.
Bone regeneration with BMP-2 delivered from keratose scaffolds
Abstract
Infuse(®) is used clinically to promote bone repair. Its efficacy is dependent on a crosslinked collagen carrier/scaffold system that has come under scrutiny due to an inability to control BMP-2 release, which may result in unwanted outcomes such as heterotopic ossification. In this study, keratose biomaterial was evaluated as a new carrier/scaffold. Keratose was mixed with BMP-2, fabricated into a scaffold, and implanted into a critical-size rat femoral defect. This construct showed bridging as early as 4 weeks and induced trabecular morphology characteristic of a remodeling hard fracture callus at 16 weeks. Compared to the normal cortical bone, the regenerated tissue had greater volume and mineral content but less density and ultimate shear stress values. Moreover, μ-CT, biomechanics, FTIR-ATR spectroscopy, and polarized light microscopy data showed regeneration using keratose was similar to an Infuse control. However, unlike Infuse's collagen carrier system, in vitro analysis showed that BMP-2 release correlated with keratose scaffold degradation. Surprisingly, treatment with keratose only led to deposition of more bone outgrowth than the untreated negative control at the 8-week time point. The application of keratose also demonstrated a notable reduction of adipose tissues within the gap. While not able to induce osteogenesis on its own, keratose may be the first biomaterial capable of suppressing adipose tissue formation, thereby indirectly enhancing bone regeneration.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“We confirmed previous studies showing that keratin scaffolds lacking rhBMP-2 do not lead to bridging in this critically-sized defected model.”
From Keratin Scaffold Formulation Impacts rhBMP-2 Biodistribution and Bone Regeneration in a Rat Femur Defect Model (2025) · cites this paper as [16]
Citations
Cites
GOVENDER, S., CSIMMA, C., GENANT, H. K., & VALENTIN-OPRAN, A. (2002) · The Journal of Bone and Joint Surgery-American Volume · doi:10.2106/00004623-200212000-00001 · Cited by 1,308
A review of polymer dissolution
Miller-Chou, B. A., & Koenig, J. L. (2003) · Progress in Polymer Science · doi:10.1016/s0079-6700(03)00045-5 · Cited by 1,159
Collagen sponges for bone regeneration with rhBMP-2
Geiger, M. (2003) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2003.08.010 · Cited by 762
FTIR and XRD evaluation of carbonated hydroxyapatite powders synthesized by wet methods
Slosarczyk, A., Paszkiewicz, Z., & Paluszkiewicz, C. (2005) · Journal of Molecular Structure · doi:10.1016/j.molstruc.2004.11.078 · Cited by 481
JONES, A. L., Bucholz, R. W., Bosse, M. J., Mirza, S. K., Lyon, T. R., Webb, L. X., Pollak, A. N., Golden, J. D., & Valentin-Opran, A. (2006) · Journal of Bone and Joint Surgery · doi:10.2106/jbjs.e.00381 · Cited by 444
The management of fractures with bone loss
Keating, J. F., Simpson, H., & Robinson, C. M. (2005) · Journal of Bone and Joint Surgery - British Volume · doi:10.1302/0301-620x.87b2.15874 · Cited by 425
Fiedler, J., Röderer, G., Günther, K., & Brenner, R. E. (2002) · Journal of Cellular Biochemistry · doi:10.1002/jcb.10309 · Cited by 395
Hill, P. S., Garrett, J. P., Ma, J., Apel, P. J., Klorig, D. C., Smith, T. L., Koman, L. A., Atala, A., & Dyke, M. E. V. (2007) · Biomaterials · doi:10.1016/j.biomaterials.2007.08.023 · Cited by 346
Self-citationWong, D. A., Kumar, A., Jatana, S., Ghiselli, G., & Wong, K. (2007) · The Spine Journal · doi:10.1016/j.spinee.2007.06.014 · Cited by 320
Recombinant Human Bone Morphogenetic Protein-2 for Treatment of Open Tibial Fractures
Starr, A. J. (2003) · Journal of Bone and Joint Surgery · doi:10.2106/00004623-200310000-00027 · Cited by 297
Research works printed but not linked: 2
- Schwartz, 2006, Segmental bone defects treated using recombinant human bone morphogenetic protein, J Orthop, 3, e2
- Mann, 1989, Bone healing, Semin Vet Med Surg, 4, 312
Cited by
Sorted by citations
Keratin: dissolution, extraction and biomedical application
Shavandi, A., Silva, T. H., Bekhit, A. A., & Bekhit, A. E.-D. A. (2017) · Biomaterials Science · doi:10.1039/c7bm00411g · Cited by 552
Keratin - Based materials for biomedical applications
Feroz, S., Muhammad, N., Ratnayake, J. T., & Dias, G. J. (2020) · Bioactive Materials · doi:10.1016/j.bioactmat.2020.04.007 · Cited by 389
Shen, X., Zhang, Y., Gu, Y., Xu, Y. L., Liu, Y., Li, B., & Chen, L. (2016) · Biomaterials · doi:10.1016/j.biomaterials.2016.08.023 · Cited by 251
The current state of scaffolds for musculoskeletal regenerative applications
Smith, B. D., & Grande, D. A. (2015) · Nature Reviews Rheumatology · doi:10.1038/nrrheum.2015.27 · Cited by 207
Understanding interactions between biomaterials and biological systems using proteomics
Othman, Z., Cillero‐Pastor, B., van Rijt, S., & Habibović, P. (2018) · Biomaterials · doi:10.1016/j.biomaterials.2018.03.020 · Cited by 214
Metadata
- Authors
- de Guzman, R. C., Saul, J. M., Ellenburg, M. D., Merrill, M. R., Coan, H. B., Smith, T. L., & Van Dyke, M. E.
- Affiliations
- Miami University (US)
- Wake Forest University Health Sciences (US)
- Venue
- Biomaterials
- Publisher
- Elsevier BV
- Published
- 2013-02
- Volume
- 34
- Issue
- 6
- Pages
- 1644-1656
- ISSN
- 0142-9612 (ISSN-L)
- OpenAlex
- W2024743274
- Cited by
- 147
- References
- 45
- Citations by year
- 2026: 2 · 2025: 5 · 2024: 7 · 2023: 8 · 2022: 7 · 2021: 9 · 2020: 15 · 2019: 13 · 2018: 16 · 2017: 19 · 2016: 14 · 2015: 12 · 2014: 13 · 2013: 7
- FWCI
- 5.83
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biomaterials (publishedVersion)
- PubMed (publishedVersion)
- Funders
- US Army
- KeraNetics, LLC
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Health Sciences › Medicine › Orthopedics and Sports Medicine › Bone health and osteoporosis research
- Keywords
- bone regeneration, BMP-2, keratosis, bone graft substitutes, scaffold degradation, critical-size femoral defect, heterotopic ossification, trabecular bone remodeling, bone biomechanics, collagen carrier
- MeSH terms
- Animals, Biomechanical Phenomena, Bone and Bones, Regeneration, Spectrophotometry, Infrared, Rats, Tissue Scaffolds, Bone Morphogenetic Protein 2
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
