Biomimetic 3D tissue printing for soft tissue regeneration
Abstract
Engineered adipose tissue constructs that are capable of reconstructing soft tissue with adequate volume would be worthwhile in plastic and reconstructive surgery. Tissue printing offers the possibility of fabricating anatomically relevant tissue constructs by delivering suitable matrix materials and living cells. Here, we devise a biomimetic approach for printing adipose tissue constructs employing decellularized adipose tissue (DAT) matrix bioink encapsulating human adipose tissue-derived mesenchymal stem cells (hASCs). We designed and printed precisely-defined and flexible dome-shaped structures with engineered porosity using DAT bioink that facilitated high cell viability over 2 weeks and induced expression of standard adipogenic genes without any supplemented adipogenic factors. The printed DAT constructs expressed adipogenic genes more intensely than did non-printed DAT gel. To evaluate the efficacy of our printed tissue constructs for adipose tissue regeneration, we implanted them subcutaneously in mice. The constructs did not induce chronic inflammation or cytotoxicity postimplantation, but supported positive tissue infiltration, constructive tissue remodeling, and adipose tissue formation. This study demonstrates that direct printing of spatially on-demand customized tissue analogs is a promising approach to soft tissue regeneration.
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Immunostaining both native and decellularized tissue sections with type IV collagen antibodies, coupled with DAPI counterstaining, is used to visualize cell nuclei and confirm decellularization efficacy and ECM component preservation.
“Immunostaining both native and decellularized tissue sections with type IV collagen antibodies, coupled with DAPI counterstaining, is used to visualize cell nuclei and confirm decellularization efficacy and ECM component preservation.”
From Bioactive scaffolds for tissue engineering: A review of decellularized extracellular matrix applications and innovations (2025) · cites this paper as [93]
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- Authors
- Pati, F., Ha, D.-H., Jang, J., Han, H. H., Rhie, J.-W., & Cho, D.-W.
- Affiliations
- Pohang University of Science and Technology (KR)
- Catholic University of Korea (KR)
- Corresponding author
- Cho, Dong-Woo
- Venue
- Biomaterials
- Publisher
- Elsevier BV
- Published
- 2015-09
- Volume
- 62
- Pages
- 164-175
- ISSN
- 0142-9612 (ISSN-L)
- OpenAlex
- W1810095863
- Cited by
- 368
- References
- 2
- Citations by year
- 2026: 9 · 2025: 21 · 2024: 29 · 2023: 23 · 2022: 28 · 2021: 34 · 2020: 45 · 2019: 51 · 2018: 45 · 2017: 37 · 2016: 42 · 2015: 4
- FWCI
- 21.23
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biomaterials (publishedVersion)
- PubMed (publishedVersion)
- Open Access System for Information Sharing (Pohang University of Science and Technology) (submittedVersion)
- Funders
- National Research Foundation of Korea (2010-0018294)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
- Keywords
- soft tissue regeneration, adipose tissue engineering, decellularized adipose tissue, bioinks, adipose-derived stem cells, adipogenesis, subcutaneous implantation, tissue remodeling, biomimetic scaffolds
- MeSH terms
- Adipose Tissue, Animals, Cell Differentiation, Cells, Cultured, Equipment Design, Mice, Nude, Regeneration, Adipocytes, Equipment Failure Analysis, Biomimetics, Mesenchymal Stem Cell Transplantation, Guided Tissue Regeneration, Adipogenesis, Mice, Tissue Scaffolds, Mesenchymal Stem Cells, Printing, Three-Dimensional
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