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Ethanolic extract of Pyrrosia lingua (Thunb.) Farw. ameliorates OVX-induced bone loss and RANKL-induced osteoclastogenesis

Jang, Seon-A, Hwang, Youn-Hwan, Yang, Hyun, Ryuk, Jin Ah, Gu, Dong Ryun, Ha, Hyunil·2022·Biomedicine & Pharmacotherapy
DOI 10.1016/j.biopha.2022.112640
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Fig. 1 Inhibitory effect of an ethanolic extract of P. lingua (EEPL) on osteoclast differentiation. (A) Cell viability after treatment of bone marrow macrophages (BMMs) with EEPL. (B) Effect of EEPL on osteoclast differentiation in MLO-Y4-BMM co-culture system. Representative images of tartrate-resistant acid phosphatase (TRAP) staining (upper panel; scale bar, 200 µ m) and the number of TRAP-positive multinucleated cells (MNCs) (lower panel) with more than three nuclei. (C) Effect of EEPL on osteoclast differentiation in BMMs. Representative images of TRAP staining (left panel; scale bar, 200 µ m), TRAP activity (middle panel), and the number of TRAP-positive MNCs (right panel) with more than three nuclei. * p < 0.05, * * p < 0.01 vs. control.
Fig. 1 Inhibitory effect of an ethanolic extract of P. lingua (EEPL) on osteoclast differentiation. (A) Cell viability after treatment of bone marrow macrophages (BMMs) with EEPL. (B) Effect of EEPL on osteoclast differentiation in MLO-Y4-BMM co-culture system. Representative images of tartrate-resistant acid phosphatase (TRAP) staining (upper panel; scale bar, 200 µ m) and the number of TRAP-positive multinucleated cells (MNCs) (lower panel) with more than three nuclei. (C) Effect of EEPL on osteoclast differentiation in BMMs. Representative images of TRAP staining (left panel; scale bar, 200 µ m), TRAP activity (middle panel), and the number of TRAP-positive MNCs (right panel) with more than three nuclei. * p < 0.05, * * p < 0.01 vs. control.CC-BY-NC-ND · From the PDF
Image 3
CC-BY-NC-ND · From the PDF
Fig. 3 Inhibitory effect of EEPL on bone loss in OVX mice. Sham or ovariectomy (OVX) mice were treated with vehicle or EEPL for 5 weeks. (A) Micro-computed tomography images (scale bar 0.5 mm) showing bone mineral density (BMD) and bone morphometric parameters of the trabecular bone. OVX, ovariectomy; EEPL-L, OVX mice fed EEPL 30 mg/kg/day; EEPL-H, OVX mice fed EEPL 100 mg/kg/day; BV/TV, bone volume per tissue volume; Tb.N, trabecular number; Tb.Sp, trabecular separation; Tb.Th, trabecular thickness. (B) Serum levels of CTX-1. CTX-I, C-terminal cross-linked telopeptides of type I collagen. (C) Body weight gain and uterine weight. * p < 0.05, * * p < 0.01 vs. the OVX group.
Fig. 3 Inhibitory effect of EEPL on bone loss in OVX mice. Sham or ovariectomy (OVX) mice were treated with vehicle or EEPL for 5 weeks. (A) Micro-computed tomography images (scale bar 0.5 mm) showing bone mineral density (BMD) and bone morphometric parameters of the trabecular bone. OVX, ovariectomy; EEPL-L, OVX mice fed EEPL 30 mg/kg/day; EEPL-H, OVX mice fed EEPL 100 mg/kg/day; BV/TV, bone volume per tissue volume; Tb.N, trabecular number; Tb.Sp, trabecular separation; Tb.Th, trabecular thickness. (B) Serum levels of CTX-1. CTX-I, C-terminal cross-linked telopeptides of type I collagen. (C) Body weight gain and uterine weight. * p < 0.05, * * p < 0.01 vs. the OVX group.CC-BY-NC-ND · From the PDF
Fig. 4 UHPLC -MS/MS analysis of EEPL. (A) Base peak chromatogram. (B) Extracted ion chromatogram of identified phytochemicals. 1, quinic acid; 2, neo­ chlorogenic acid; 3, chlorogenic acid; 4, caffeic acid; 5, orientin; 6, vitexin; 7, isochlorogenic acid; 8, quercetin; 9, kaempferol; 10, mangiferin; 11, isomangiferin; 12, vitexin glucoside; 13, narirutin.
Fig. 4 UHPLC -MS/MS analysis of EEPL. (A) Base peak chromatogram. (B) Extracted ion chromatogram of identified phytochemicals. 1, quinic acid; 2, neo­ chlorogenic acid; 3, chlorogenic acid; 4, caffeic acid; 5, orientin; 6, vitexin; 7, isochlorogenic acid; 8, quercetin; 9, kaempferol; 10, mangiferin; 11, isomangiferin; 12, vitexin glucoside; 13, narirutin.CC-BY-NC-ND · From the PDF

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Metadata

Authors
Jang, S.-A., Hwang, Y.-H., Yang, H., Ryuk, J. A., Gu, D. R., & Ha, H.
Venue
Biomedicine & Pharmacotherapy
Publisher
Elsevier BV
Published
2022-03
Volume
147
Pages
112640
ISSN
0753-3322
DOI
10.1016/j.biopha.2022.112640
Cited by
4
References
2
Language
en
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CoChat. (2026). Citation check: Ethanolic extract of Pyrrosia lingua (Thunb.) Farw. ameliorates OVX-induced bone loss and RANKL-induced osteoclastogenesis. https://app.cochat.ai/papers/10.1016/j.biopha.2022.112640
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Jang, S.-A., Hwang, Y.-H., Yang, H., Ryuk, J. A., Gu, D. R., & Ha, H. (2022). Ethanolic extract of Pyrrosia lingua (Thunb.) Farw. ameliorates OVX-induced bone loss and RANKL-induced osteoclastogenesis. Biomedicine & Pharmacotherapy. https://doi.org/10.1016/j.biopha.2022.112640

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