Impacts of main factors on bioethanol fermentation from stalk juice of sweet sorghum by immobilized Saccharomyces cerevisiae (CICC 1308)
Abstract
In order to attain a higher ethanol yield and faster ethanol fermentation rate, orthogonal experiments of ethanol fermentation with immobilized yeast from stalk juice of sweet sorghum were carried out in the shaking flasks to investigate the effect of main factors, namely, fermentation temperature, agitation rate, particles stuffing rate and pH on ethanol yield and CO(2) weight loss rate. The range analysis and analysis of variance (ANOVA) were applied for the results of orthogonal experiments. Results showed that the optimal condition for bioethanol fermentation should be A(4)B(3)C(3)D(4), namely, fermentation temperature, agitation rate, particles stuffing rate and pH were 37 degrees C, 200rpm, 25% and 5.0, respectively. The verification experiments were carried out in shaking flasks and 5L bioreactor at the corresponding parameters. The results of verification experiments in the shaking flasks showed that ethanol yield and CO(2) weight loss rate were 98.07% and 1.020gh(-1), respectively. The results of ethanol fermentation in the 5L bioreactor showed that ethanol yield and fermentation time were 93.24% and 11h, respectively. As a result, it could be concluded that the determined optimal condition A(4)B(3)C(3)D(4) was suitable and reasonable for the ethanol fermentation by immobilized Saccharomyces cerevisiae. The conclusion in the research would be beneficial for application of ethanol fermentation by immobilized S. cerevisiae from stalk juice of sweet sorghum.
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The yield and quality of bioethanol produced is strongly dependent on the fermentation process, which is affected by temperature, pH, oxygen, substrate concentration, and the fermenter organism used.
“The yield and quality of the bioethanol produced is strongly dependent on the fermentation process, which is affected by several factors, such as temperature, pH, oxygen, substrate concentration, and the fermenter organism used [126–130].”
From The promising future of microalgae: Current status, challenges, and optimization of a sustainable and renewable industry for biofuels, feed, and other products (2018) · cites this paper as [126]
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- Authors
- LIU, R., & SHEN, F.
- Affiliations
- Shanghai Jiao Tong University (CN)
- Corresponding author
- LIU, R
- ORCID
- LIU, R
- Venue
- Bioresource Technology
- Publisher
- Elsevier BV
- Published
- 2008-03
- Volume
- 99
- Issue
- 4
- Pages
- 847-854
- ISSN
- 0960-8524 (ISSN-L)
- OpenAlex
- W2039700620
- Cited by
- 173
- References
- 15
- Citations by year
- 2026: 3 · 2025: 7 · 2024: 5 · 2023: 21 · 2022: 5 · 2021: 7 · 2020: 9 · 2019: 8 · 2018: 6 · 2017: 9 · 2016: 5 · 2015: 7 · 2014: 15 · 2013: 8 · 2012: 20
- FWCI
- 4.54
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Bioresource Technology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Shanghai Jiao Tong University
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
- Physical Sciences › Engineering › Building and Construction › Anaerobic Digestion and Biogas Production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Keywords
- bioethanol fermentation, immobilized yeast, Saccharomyces cerevisiae, fermentation optimization, ethanol yield, fermentation temperature, stirring speed, pH
- MeSH terms
- Ethanol, Plant Extracts, Saccharomyces cerevisiae, Cells, Immobilized, Cell Culture Techniques, Bioreactors, Plant Components, Aerial, Sorghum
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