Saccharification of Spirulina Platensis biomass using free and immobilized amylolytic enzymes
Abstract
We aimed to use physical methods of microalgal biomass rupture to study saccharification strategies using free and immobilized amylolytic enzymes. The biomass of Spirulina platensis, which consists of 50-60% carbohydrates, was exposed to physical cell rupture treatments, with better results obtained using freeze/thaw cycles following by gelatinization. In saccharification tests, it was possible to hydrolyze Spirulina biomass with hydrolysis efficiencies above 99% and 83%, respectively, using 1% (v/v) of free enzymes or 1% (m/v) of amylolytic enzymes immobilized together. The use of free and immobilized enzymes yielded high levels of conversion of polysaccharides to simple sugars in Spirulina biomass, showing that these processes are promising for the advancement of bioethanol production using microalgal biomass.
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The intracellular carbohydrates of microalgae are utilized to produce bioethanol.
“The intracellular carbohydrates of microalgae are utilized to produce bioethanol [1,8].”
From Strategy Development for Microalgae Spirulina platensis Biomass Cultivation in a Bubble Photobioreactor to Promote High Carbohydrate Content (2022) · cites this paper as [8]
Cites
PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT
Lowry, O. H., Rosebrough, N., Farr, A. L., & Randall, R. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)52451-6 · Cited by 315,771 · see citing works
Official Methods of Analysis of
Helrick, K. (1980) · Analytical Chemistry · doi:10.1021/ac50052a726 · Cited by 59,351 · see citing works
Colorimetric Method for Determination of Sugars and Related Substances
DuBois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A., & Smith, F. (1956) · Analytical Chemistry · doi:10.1021/ac60111a017 · Cited by 52,269 · see citing works
Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar
Miller, G. L. (1959) · Analytical Chemistry · doi:10.1021/ac60147a030 · Cited by 28,963 · see citing works
(2023) · Oxford University Press eBooks · doi:10.1093/9780197610145.002.001 · Cited by 28,120 · see citing works
Chen, C.-Y., Yeh, K.-L., Aisyah, R., Lee, D.-J., & Chang, J.-S. (2011) · Bioresource Technology · doi:10.1016/j.biortech.2010.06.159 · Cited by 1,860 · see citing works
Micro and macroalgal biomass: a renewable source for bioethanol
John, R. P., Anisha, G. S., Nampoothiri, K. M., & Pandey, A. (2011) · Bioresource Technology · doi:10.1016/j.biortech.2010.06.139 · Cited by 1,090 · see citing works
Aquatic phototrophs: efficient alternatives to land-based crops for biofuels
Dismukes, G. C., Carrieri, D., Bennette, N., Ananyev, G. M., & Posewitz, M. C. (2008) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2008.05.007 · Cited by 733 · see citing works
Microalgae-based carbohydrates for biofuel production
Chen, C.-Y., Zhao, X.-Q., Yen, H.-W., Ho, S.-H., Cheng, C.-L., Lee, D.-J., Bai, F.-W., & Chang, J.-S. (2013) · Biochemical Engineering Journal · doi:10.1016/j.bej.2013.03.006 · Cited by 724 · see citing works
Challenges and opportunities in improving the production of bio-ethanol
Baeyens, J., Kang, Q. K., Appels, L., Dewil, R., Lv, Y., & Tan, T. (2014) · Progress in Energy and Combustion Science · doi:10.1016/j.pecs.2014.10.003 · Cited by 569 · see citing works
Markou, G., Angelidaki, I., & Georgakakis, D. (2012) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-012-4398-0 · Cited by 549 · see citing works
Microbial cellulases: Engineering, production and applications
Juturu, V., & Wu, J. C. (2014) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2014.01.077 · Cited by 493 · see citing works
Mechanism and challenges in commercialisation of algal biofuels
Singh, A., Nigam, P. S., & Murphy, J. D. (2011) · Bioresource Technology · doi:10.1016/j.biortech.2010.06.057 · Cited by 476 · see citing works
Enzymatic pretreatment of Chlamydomonas reinhardtii biomass for ethanol production
Choi, S. P., Nguyen, M. T., & Sim, S. J. (2010) · Bioresource Technology · doi:10.1016/j.biortech.2010.02.026 · Cited by 408 · see citing works
Challenges with nonfiber carbohydrate methods1,2
Hall, M. B. (2003) · Journal of Animal Science · doi:10.2527/2003.81123226x · Cited by 318 · see citing works
Hernández, D., Riaño, B., Coca, M., & García-González, M. C. (2014) · Chemical Engineering Journal · doi:10.1016/j.cej.2014.10.049 · Cited by 252 · see citing works
Spectrophotometric Method for Hydroxymethylfurfural in Honey
WHITE, J. W. (1979) · Journal of AOAC INTERNATIONAL · doi:10.1093/jaoac/62.3.509 · Cited by 236 · see citing works
Progress of microalgae biofuel’s commercialization
Su, Y., Song, K., Zhang, P., Su, Y., Cheng, J. Q., & Chen, X. (2017) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2016.12.078 · Cited by 229 · see citing works
Assessing the potential of algal biomass opportunities for bioenergy industry: A review
Ullah, K., Ahmad, M., Sofia, Sharma, V. K., Lu, P., Harvey, A., Zafar, M., & Sultana, S. (2015) · Fuel · doi:10.1016/j.fuel.2014.10.064 · Cited by 211 · see citing works
Bioethanol Production by Carbohydrate-Enriched Biomass of Arthrospira (Spirulina) platensis
Μάρκου, Γ., Angelidaki, İ., Nerantzis, E. T., & Georgakakis, D. (2013) · Energies · doi:10.3390/en6083937 · Cited by 207 · see citing works
Chagas, B. M. E., Dorado, C., Serapiglia, M. J., Mullen, C. A., Boateng, A. A., Melo, M. A. F., & Ataíde, C. H. (2016) · Fuel · doi:10.1016/j.fuel.2016.03.076 · Cited by 168 · see citing works
Opportunities for utilization of non-conventional energy sources for biomass pretreatment
Singh, R., Krishna, B. B., Kumar, J., & Bhaskar, T. (2015) · Bioresource Technology · doi:10.1016/j.biortech.2015.08.117 · Cited by 162 · see citing works
Optimizing microalgae cultivation and wastewater treatment in large-scale offshore photobioreactors
Novoveská, L., Zapata, A. K. M., Zabolotney, J. B., Atwood, M. C., & Sundstrom, E. R. (2016) · Algal Research · doi:10.1016/j.algal.2016.05.033 · Cited by 162 · see citing works
Lee, O. K., Kim, A. L., Seong, D. H., Lee, C., Jung, Y. T., Lee, J. W., & Lee, E. Y. (2013) · Bioresource Technology · doi:10.1016/j.biortech.2013.01.007 · Cited by 155 · see citing works
Colla, L., Bertolin, T., & Costa, J. (2004) · Zeitschrift für Naturforschung C - A Journal of Biosciences · doi:10.1515/znc-2004-1-212 · Cited by 120 · see citing works
Self-citation‘Pilot scale semicontinuous production of Spirulina biomass in southern Brazil’.
Morais, M. G., Radmann, E. M., Andrade, M. R., Teixeira, G. G., Brusch, L. R. F., & Costa, J. A. V. (2009) · Aquaculture · doi:10.1016/j.aquaculture.2009.05.009 · Cited by 114 · see citing works
Solar biofuels production with microalgae
Hallenbeck, P. C., Grogger, M. L. M., Mraz, M., & Veverka, D. (2016) · Applied Energy · doi:10.1016/j.apenergy.2016.06.024 · Cited by 109 · see citing works
Salla, A. C. V., Margarites, A. C., Seibel, F., Holz, L. C., Brião, V. B., Bertolin, T. E., Colla, L. M., & Costa, J. A. V. (2016) · Bioresource Technology · doi:10.1016/j.biortech.2016.02.069 · Cited by 106 · see citing works
Self-citationThe Enzymatic Conversion of Major Algal and Cyanobacterial Carbohydrates to Bioethanol
Al Abdallah, Q., Nixon, B. T., & Fortwendel, J. R. (2016) · Frontiers in Energy Research · doi:10.3389/fenrg.2016.00036 · Cited by 102 · see citing works
Usha, M. T., Chandra, T. S., Sarada, R., & Chauhan, V. S. (2016) · Bioresource Technology · doi:10.1016/j.biortech.2016.04.060 · Cited by 90 · see citing works
Enhanced enzymatic conversion with freeze pretreatment of rice straw
Chang, K., Thitikorn-amorn, J., Hsieh, J., Ou, B.-M., Chen, S.-H., Ratanakhanokchai, K., Huang, P., & Chen, S. (2010) · Biomass and Bioenergy · doi:10.1016/j.biombioe.2010.08.027 · Cited by 89 · see citing works
Preparation of core–shell polymer supports to immobilize lipase B from Candida antarctica
Cunha, A. G., Ribeiro, M. D. B., Manoel, E. A., et al. (2013) · Journal of Molecular Catalysis B Enzymatic · doi:10.1016/j.molcatb.2013.11.020 · Cited by 88 · see citing works
Alva, Anupama, Savla, et al. (2007) · AFRICAN JOURNAL OF BIOTECHNOLOGY · doi:10.5897/ajb2007.000-2050 · Cited by 87 · see citing works
Pancha, I., Chokshi, K., Maurya, R., Bhattacharya, S., Bachani, P., & Mishra, S. (2016) · Bioresource Technology · doi:10.1016/j.biortech.2015.12.078 · Cited by 67 · see citing works
Abdullah, R., Ueda, K., & Saka, S. (2014) · Journal of Wood Science · doi:10.1007/s10086-014-1401-7 · Cited by 62 · see citing works
Canter, C. E., Blowers, P., Handler, R. M., & Shonnard, D. R. (2015) · Applied Energy · doi:10.1016/j.apenergy.2014.12.065 · Cited by 54 · see citing works
[Biological activity of Spirulina].
Блинкова, Л. П., Gorobets, O., & Baturo, A. P. (2001) · PubMed · Cited by 52 · see citing works
(2012) · Cited by 51 · see citing works
El‐Mashad, H. M. (2014) · Biochemical Engineering Journal · doi:10.1016/j.bej.2014.09.009 · Cited by 50 · see citing works
Autotrophic production of biodiesel from microalgae: An updated process and economic analysis
Tercero, E. A. R., Domenicali, G., & Bertucco, A. (2014) · Energy · doi:10.1016/j.energy.2014.08.077 · Cited by 50 · see citing works
An overview of holocellulose-degrading enzyme immobilization for use in bioethanol production
Vaz, R. P., Moreira, L. R. D. S., & Filho, E. X. F. (2016) · Journal of Molecular Catalysis B Enzymatic · doi:10.1016/j.molcatb.2016.08.006 · Cited by 48 · see citing works
Pejin, J. D., Mojović, L. V., Pejin, D. J., Kocić‐Tanackov, S., Savić, D. S., Nikolić, S. B., & Djukić‐Vuković, A. (2014) · Fuel · doi:10.1016/j.fuel.2014.10.077 · Cited by 47 · see citing works
Production and purification of amylolytic enzymes for saccharification of microalgal biomass
Rodrigues, É. F., FICANHA, A. M. M., Dallago, R. M., Treichel, H., Reinehr, C. O., Machado, T. P., Nunes, G. B., & Colla, L. M. (2016) · Bioresource Technology · doi:10.1016/j.biortech.2016.11.047 · Cited by 37 · see citing works
Self-citationTuankriangkrai, S., & Benjakul, S. (2010) · Journal of Muscle Foods · doi:10.1111/j.1745-4573.2009.00190.x · Cited by 32 · see citing works
Bhagawan, S. (2007) · Cited by 32 · see citing works
Magro, F. G., Margarites, A. C., Reinehr, C. O., Gonçalves, G. C., Rodigheri, G., Costa, J. A. V., & Colla, L. M. (2017) · Environmental Technology · doi:10.1080/09593330.2017.1340352 · Cited by 29 · see citing works
Self-citationVaithanomsat, P., Kosugi, A., Apiwatanapiwat, W., et al. (2012) · Bioresource Technology · doi:10.1016/j.biortech.2012.11.023 · Cited by 15 · see citing works
Vargas, C. E. B., de Olíveira, D., Valduga, E., Venquiaruto, L. D., Paroul, N., Backes, G. T., & Dallago, R. M. (2016) · Applied Biochemistry and Biotechnology · doi:10.1007/s12010-016-2050-7 · Cited by 8 · see citing works
Gomes, D. Í., Sampaio, C. B., Detmann, E., Filho, S. D. C. V., Mezzomo, R., & Filho, J. G. L. R. (2014) · Semina Ciências Agrárias · doi:10.5433/1679-0359.2014v35n4suplp2629 · Cited by 4 · see citing works
Starch hydrolysis by the synergistic action of amylase and glucoamylase
Presečki, A. V., Findrik, Z., & Vasić‐Rački, Đ. (2009) · New Biotechnology · doi:10.1016/j.nbt.2009.06.1003 · Cited by 2 · see citing works
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Cited by
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Nazari, M. T., Mazutti, J., Basso, L. G., Colla, L. M., & Brandli, L. (2021) · Environment Development and Sustainability · doi:10.1007/s10668-020-01110-4 · Cited by 127 · see citing works
Self-citationMicroalgae Starch: A Promising Raw Material for the Bioethanol Production
Maia, J. L. D., Cardoso, J. S., Mastrantonio, D. J. D. S., Bierhals, C. K., Moreira, J. B., Costa, J. A. V., & Morais, M. G. D. (2020) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2020.10.159 · Cited by 116 · see citing works
Saxena, R., Rodríguez-Jasso, R. M., Chávez-Gonzalez, M. L., Aguilar, C. N., Quijano, G., & Ruiz, H. A. (2022) · Fermentation · doi:10.3390/fermentation8080374 · Cited by 44 · see citing works
Bioethanol from hydrolyzed Spirulina (Arthrospira Platensis) biomass using ethanologenic bacteria
Werlang, E. B., Julich, J., Muller, M. V. G., de Farias Neves, F., Sierra-Ibarra, E., Martinez, A., & Schneider, R. D. C. D. S. (2020) · Bioresources and Bioprocessing · doi:10.1186/s40643-020-00315-9 · Cited by 26 · see citing works
- Authors
- Rempel, A., Machado, T., Treichel, H., Colla, E., Margarites, A. C., & Colla, L. M.
- Affiliations
- Universidade de Passo Fundo (BR)
- Universidade Federal da Fronteira Sul (BR)
- Universidade Tecnológica Federal do Paraná (BR)
- Corresponding author
- Colla, Eliane
- Colla, Luciane Maria
- Venue
- Bioresource Technology
- Publisher
- Elsevier BV
- Published
- 2018-09
- Volume
- 263
- Pages
- 163-171
- ISSN
- 0960-8524 (ISSN-L)
- OpenAlex
- W2801892898
- Cited by
- 53
- References
- 50
- Citations by year
- 2026: 6 · 2025: 7 · 2024: 7 · 2023: 3 · 2022: 6 · 2021: 9 · 2020: 10 · 2019: 5
- FWCI
- 2.40
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Bioresource Technology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Conselho Nacional de Desenvolvimento Científico e Tecnológico (406915/2013-0)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Enzyme Catalysis and Immobilization
- Keywords
- Spirulina platensis, microalgal biomass, saccharification, amylolytic enzymes, enzyme immobilization, freeze-thaw cycles, gelatinization, polysaccharide hydrolysis, bioethanol production, microalgal biofuels
- MeSH terms
- Enzymes, Immobilized, Hydrolysis, Biomass, Spirulina, Microalgae
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