Cited by 53Biofuel production and bioconversionAlgal biology and biofuel productionEnzyme Catalysis and Immobilization

Saccharification of Spirulina Platensis biomass using free and immobilized amylolytic enzymes

Rempel, Alan, Machado, Tainara, Treichel, Helen, Colla, Eliane, Margarites, Ana Cláudia, Colla, Luciane Maria·2018·Bioresource Technology

Abstract

We aimed to use physical methods of microalgal biomass rupture to study saccharification strategies using free and immobilized amylolytic enzymes. The biomass of Spirulina platensis, which consists of 50-60% carbohydrates, was exposed to physical cell rupture treatments, with better results obtained using freeze/thaw cycles following by gelatinization. In saccharification tests, it was possible to hydrolyze Spirulina biomass with hydrolysis efficiencies above 99% and 83%, respectively, using 1% (v/v) of free enzymes or 1% (m/v) of amylolytic enzymes immobilized together. The use of free and immobilized enzymes yielded high levels of conversion of polysaccharides to simple sugars in Spirulina biomass, showing that these processes are promising for the advancement of bioethanol production using microalgal biomass.

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Crossref · 10.1016/j.biortech.2018.04.114

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Authors
Rempel, A., Machado, T., Treichel, H., Colla, E., Margarites, A. C., & Colla, L. M.
Affiliations
  • Universidade de Passo Fundo (BR)
  • Universidade Federal da Fronteira Sul (BR)
  • Universidade Tecnológica Federal do Paraná (BR)
Corresponding author
  • Colla, Eliane
  • Colla, Luciane Maria
Venue
Bioresource Technology
Publisher
Elsevier BV
Published
2018-09
Volume
263
Pages
163-171
ISSN
0960-8524 (ISSN-L)
OpenAlex
W2801892898
Cited by
53
References
50
Citations by year
2026: 6 · 2025: 7 · 2024: 7 · 2023: 3 · 2022: 6 · 2021: 9 · 2020: 10 · 2019: 5
FWCI
2.40
Type
journal article
Language
en
Open access
closed
Locations
  • Bioresource Technology (publishedVersion)
  • PubMed (publishedVersion)
Funders
Conselho Nacional de Desenvolvimento Científico e Tecnológico (406915/2013-0)
Topics
  • Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
  • Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Enzyme Catalysis and Immobilization
Keywords
Spirulina platensis, microalgal biomass, saccharification, amylolytic enzymes, enzyme immobilization, freeze-thaw cycles, gelatinization, polysaccharide hydrolysis, bioethanol production, microalgal biofuels
MeSH terms
Enzymes, Immobilized, Hydrolysis, Biomass, Spirulina, Microalgae

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