Enzymatic pretreatment of Chlamydomonas reinhardtii biomass for ethanol production
Abstract
The production of ethanol from feedstock other than agriculture materials has been promoted in recent years. Some microalgae can accumulate a high starch content (about 44% of dry base) via photosynthesis. Algal biomass, Chlamydomonas reinhardtii UTEX 90, was converted into a suitable fermentable feedstock by two commercial hydrolytic enzymes. The results showed that almost all starch was released and converted into glucose without steps for the cell wall disruption. Various conditions in the liquefaction and saccharification processes, such as enzyme concentration, pH, temperature, and residence time, have been investigated to obtain an optimum combination using the orthogonal analysis. As a result, approximately 235 mg of ethanol was produced from 1.0 g of algal biomass by a separate hydrolysis and fermentation (SHF) method. The main advantages of this process include the low cost of chemicals, short residence time, and simple equipment system, all of which promote its large-scale application.
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Enzymatic pretreatment of algal biomass is more productive and advantageous for fermentation processes.
“Enzymatic pretreatment of algal biomass is more productive and advantageous for fermentation processes [118, 119].”
From The promising future of microalgae: Current status, challenges, and optimization of a sustainable and renewable industry for biofuels, feed, and other products (2018) · cites this paper as [118]
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- Authors
- Choi, S. P., Nguyen, M. T., & Sim, S. J.
- Affiliations
- Sungkyunkwan University (KR)
- Corresponding author
- Sim, Sang Jun
- Venue
- Bioresource Technology
- Publisher
- Elsevier BV
- Published
- 2010-07
- Volume
- 101
- Issue
- 14
- Pages
- 5330-5336
- ISSN
- 0960-8524 (ISSN-L)
- OpenAlex
- W2005392171
- Cited by
- 408
- References
- 26
- Citations by year
- 2026: 7 · 2025: 22 · 2024: 9 · 2023: 32 · 2022: 31 · 2021: 36 · 2020: 36 · 2019: 39 · 2018: 25 · 2017: 26 · 2016: 32 · 2015: 31 · 2014: 25 · 2013: 26 · 2012: 21
- FWCI
- 10.76
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Bioresource Technology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Physical Sciences › Engineering › Building and Construction › Anaerobic Digestion and Biogas Production
- Keywords
- Chlamydomonas reinhardtii, algal biomass, enzymatic pretreatment, ethanol production, starch hydrolysis, separate hydrolysis and fermentation, liquefaction, saccharification, hydrolytic enzymes, orthogonal experimental design
- MeSH terms
- Ethanol, Biotechnology, Carbohydrates, Enzymes, Fermentation, Glucose, Hydrogen-Ion Concentration, Hydrolysis, Photosynthesis, Temperature, Chlamydomonas reinhardtii, Biomass, Eukaryota
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