Cited by 408Biofuel production and bioconversionAlgal biology and biofuel productionAnaerobic Digestion and Biogas Production

Enzymatic pretreatment of Chlamydomonas reinhardtii biomass for ethanol production

Choi, Seung Phill, Nguyen, Minh Thu, Sim, Sang Jun·2010·Bioresource Technology

Abstract

The production of ethanol from feedstock other than agriculture materials has been promoted in recent years. Some microalgae can accumulate a high starch content (about 44% of dry base) via photosynthesis. Algal biomass, Chlamydomonas reinhardtii UTEX 90, was converted into a suitable fermentable feedstock by two commercial hydrolytic enzymes. The results showed that almost all starch was released and converted into glucose without steps for the cell wall disruption. Various conditions in the liquefaction and saccharification processes, such as enzyme concentration, pH, temperature, and residence time, have been investigated to obtain an optimum combination using the orthogonal analysis. As a result, approximately 235 mg of ethanol was produced from 1.0 g of algal biomass by a separate hydrolysis and fermentation (SHF) method. The main advantages of this process include the low cost of chemicals, short residence time, and simple equipment system, all of which promote its large-scale application.

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Crossref · 10.1016/j.biortech.2010.02.026

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Authors
Choi, S. P., Nguyen, M. T., & Sim, S. J.
Affiliations
Sungkyunkwan University (KR)
Corresponding author
Sim, Sang Jun
Venue
Bioresource Technology
Publisher
Elsevier BV
Published
2010-07
Volume
101
Issue
14
Pages
5330-5336
ISSN
0960-8524 (ISSN-L)
OpenAlex
W2005392171
Cited by
408
References
26
Citations by year
2026: 7 · 2025: 22 · 2024: 9 · 2023: 32 · 2022: 31 · 2021: 36 · 2020: 36 · 2019: 39 · 2018: 25 · 2017: 26 · 2016: 32 · 2015: 31 · 2014: 25 · 2013: 26 · 2012: 21
FWCI
10.76
Type
journal article
Language
en
Open access
closed
Locations
  • Bioresource Technology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
  • Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
  • Physical Sciences › Engineering › Building and Construction › Anaerobic Digestion and Biogas Production
Keywords
Chlamydomonas reinhardtii, algal biomass, enzymatic pretreatment, ethanol production, starch hydrolysis, separate hydrolysis and fermentation, liquefaction, saccharification, hydrolytic enzymes, orthogonal experimental design
MeSH terms
Ethanol, Biotechnology, Carbohydrates, Enzymes, Fermentation, Glucose, Hydrogen-Ion Concentration, Hydrolysis, Photosynthesis, Temperature, Chlamydomonas reinhardtii, Biomass, Eukaryota

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