Mixotrophic microalgal-biofilm reactor augmenting biomass and biofuel productivity
Abstract
The present work aimed to evaluate the mixotrophic growth of Chlorella pyrenoidosa in a microalgal-biofilm reactor (MBR) using waste glycerol as an organic carbon source. The biomass productivity of C. pyrenoidosa (10.14 g m-2 d-1) under the mixotrophic mode was remarkably higher than that observed during the phototrophic mode (4.16 g m-2 d-1), under similar incubation conditions. The hydraulic retention time (HRT) of 6 d was found optimal for the higher productivity of microalgae in the MBR. Notably, based on biofuel quality, mixotrophically grown microalgal biomass was noted to have better suitability for biomethane production compared to biodiesel. Besides, up to 98.09, 75.74, and 55.86% removal of phosphate, nitrate, and COD, respectively, was recorded within 6 d under mixotrophic growth. Overall, the present findings magnificently demonstrate the efficient recycling of waste glycerol for higher biomass production coupled with phycoremediation using mixotrophic MBR.
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The constrained concentration of microalgae within growth media derived from wastewater poses a substantial impediment to the commercial feasibility of microalgal biofuel production.
“Nonetheless, notwithstanding these auspicious discoveries, the constrained concentration of microalgae within growth media derived from wastewater, as underscored by Rana et al. [52], poses a substantial impediment to the commercial feasibility of microalgal biofuel production.”
From A Holistic Approach to Circular Bioeconomy Through the Sustainable Utilization of Microalgal Biomass for Biofuel and Other Value-Added Products (2024) · cites this paper as [52]
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- Authors
- Rana, M. S., & Prajapati, S. K.
- Affiliations
- Indian Institute of Technology Roorkee (IN)
- Corresponding author
- Prajapati, Sanjeev Kumar
- Venue
- Bioresource Technology
- Publisher
- Elsevier BV
- Published
- 2022-07
- Volume
- 356
- Pages
- 127306
- ISSN
- 0960-8524 (ISSN-L)
- OpenAlex
- W4280566357
- Semantic Scholar
- 20a70041ef5ee8efad14a8446185fdb39247a2d1
- Cited by
- 18
- References
- 48
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 8 · 2023: 3 · 2022: 2
- FWCI
- 0.72
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Bioresource Technology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Indian Institute of Technology Roorkee
- Ministry of Education, India (PMRF-192002-1354)
- Uttarakhand State Council for Science and Technology (UCS&T/R&D-10/20-21/19072/02)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Physical Sciences › Engineering › Building and Construction › Anaerobic Digestion and Biogas Production
- Physical Sciences › Environmental Science › Environmental Chemistry › Aquatic Ecosystems and Phytoplankton Dynamics
- Keywords
- mixotrophic cultivation, Chlorella pyrenoidosa, waste glycerol, microalgae productivity, hydraulic retention time, biomethane, biodiesel, phycoremediation, nitrogen removal, phosphate removal, COD removal, biofuel production
- MeSH terms
- Chlorella, Glycerol, Biofilms, Biomass, Biofuels, Microalgae
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