Flux Balance Analysis of Photoautotrophic Metabolism
Abstract
Photosynthesis is the principal process responsible for fixation of inorganic carbon dioxide into organic molecules with sunlight as the energy source. Potentially, many chemicals could be inexpensively produced by photosynthetic organisms. Mathematical modeling of photoautotrophic metabolism is therefore important to evaluate maximum theoretical product yields and to deeply understand the interactions between biochemical energy, carbon fixation, and assimilation pathways. Flux balance analysis based on linear programming is applied to photoautotrophic metabolism. The stoichiometric network of a model photosynthetic prokaryote, Synechocystis sp. PCC 6803, has been reconstructed from genomic data and biochemical literature and coupled with a model of the photophosphorylation processes. Flux map topologies for the hetero‐, auto‐, and mixotrophic modes of metabolism under conditions of optimal growth were determined and compared. The roles of important metabolic reactions such as the glyoxylate shunt and the transhydrogenase reaction were analyzed. We also theoretically evaluated the effect of gene deletions or additions on biomass yield and metabolic flux distributions.
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Previous modelling in Synechocystis PCC6803 suggested that the lack of a completed TCA cycle does not diminish growth significantly.
“Their results suggested that the lack of a completed TCA cycle does not diminish the growth significantly in this microorganism.”
From Renewable energy from Cyanobacteria: energy production optimization by metabolic pathway engineering (2011) · cites this paper as Shastri and Morgan 2005
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- Authors
- Shastri, A. A., & Morgan, J. A.
- Affiliations
- Purdue University West Lafayette (US)
- Corresponding author
- Morgan, John A.
- ORCID
- Morgan, John A.
- Venue
- Biotechnology Progress
- Publisher
- Wiley
- Published
- 2005-01
- Volume
- 21
- Issue
- 6
- Pages
- 1617-1626
- ISSN
- 1520-6033 (ISSN-L)
- 8756-7938
- OpenAlex
- W2125760935
- Cited by
- 205
- References
- 44
- Citations by year
- 2026: 4 · 2025: 5 · 2024: 10 · 2023: 4 · 2022: 10 · 2021: 6 · 2020: 16 · 2019: 6 · 2018: 13 · 2017: 14 · 2016: 16 · 2015: 13 · 2014: 15 · 2013: 17 · 2012: 13
- FWCI
- 1.50
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biotechnology Progress (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Science Foundation
- Purdue University
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
- Keywords
- photosynthesis, Synechocystis sp. PCC 6803, flux balance analysis, photophosphorylation, heterotrophic metabolism, autotrophic metabolism, mixotrophic metabolism, glyoxylate cycle, transhydrogenase, biomass yield, metabolic flux distribution, CO2 fixation
- MeSH terms
- Biotechnology, Glyoxylates, Models, Biological, Photophosphorylation, Photosynthesis, Photobiology, Biomass, Synechocystis
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