A study of electrochemical biosensor for analysis of three-dimensional (3D) cell culture
Abstract
Cell culture has a fundamental role not only in regenerative medicine but also in biotechnology, pharmacology, impacting both drug discovery and manufacturing. Although cell culture has been generally developed for only two-dimensional (2D) culture systems, three-dimensional (3D) culture is being spotlighted as the means to mimic in vivo cellular conditions. In this study, a method for cytotoxicity assay using an electrochemical biosensor applying 3D cell culture is presented. In order to strengthen the advantage of a 3D cell culture, the experimental condition of gelation between several types of sol-gels (alginate, collagen, matrigel) and cancer cells can be optimized to make a 3D cell structure on the electrode, which will show the reproducibility of electrical measurement for long-term monitoring. Moreover, cytotoxicity test results applying this method showed IC(50) value of A549 lung cancer cells to erlotinib. Thus, this study evaluates the feasibility of application of the electrochemical biosensor for 3D cell culture to cytotoxicity assay for investigation of 3D cell response to drug compounds.
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In a 3D culture-based electrochemical sensor, the resultant change in electrical current signal is opposite to the 2D case; signal increases with cell proliferation.
“On the other hand, the resultant change in electrical current signal in 3D culture-based sensor is opposite, signal increases with cell proliferation.”
From Three-dimensional cell culture systems and their applications in drug discovery and cell-based biosensors (2014) · cites this paper as [78]
Cellular responses to anticancer drugs were successfully monitored in the 3D electrochemical biosensor.
“Cellular responses to anticancer drugs were successfully monitored.”
From Three-dimensional cell culture systems and their applications in drug discovery and cell-based biosensors (2014) · cites this paper as [78]
In a 2D culture-based electrochemical sensor, as cells proliferate on the electrode, the electrical current decreases due to increasing coverage of cells on the electrode compared to the electrode without cells.
“In the 2D culture-based sensor, as cells proliferate on the electrode, the electrical current decreased due to the increasing coverage of cells on the electrode, compared to the electrode without cells.”
From Three-dimensional cell culture systems and their applications in drug discovery and cell-based biosensors (2014) · cites this paper as [78]
Other researchers have used 3D cell-based electrochemical biosensors to quantify cancer cell resistance to drug compounds and to study cancer cell proliferation in the presence of anticancer drugs.
“Other researchers have used 3D cell-based electrochemical biosensors to quantify cancer cell resistance to drug compounds 78,95 and to study cancer cell proliferation in the presence of anticancer drugs. 94”
From Three-dimensional cell culture systems and their applications in drug discovery and cell-based biosensors (2014) · cites this paper as [78]
Only 3D cultures exhibit a hierarchical structure and cellular heterogeneity that can closely mimic in vivo cell morphology and function, cell-cell interactions, and diffusion barriers.
“only 3D cultures exhibit a hierarchical structure and cellular heterogeneity that can closely mimic in vivo cell morphology and function (proliferation, differentiation, gene expression, etc.), 78,79 cell-cell interactions, and diffusion barriers.”
From Three-dimensional cell culture systems and their applications in drug discovery and cell-based biosensors (2014) · cites this paper as [78]
Jeong et al. reported a similar design of an electrochemical biosensor using several types of sol-gels (alginate, collagen, Matrigel) to grow A549 lung cancer cells in 3D on flat electrodes.
“Jeong et al . reported a similar design of an electrochemical biosensor using several types of sol-gels (alginate, collagen, Matrigel) to grow A549 lung cancer cells in 3D on flat electrodes. 78”
From Three-dimensional cell culture systems and their applications in drug discovery and cell-based biosensors (2014) · cites this paper as [78]
3D models can better replicate intrinsic physiological conditions and in vivo cellular responses to external stimuli compared to the 2D monolayer.
“Therefore, 3D models can better replicate intrinsic physiological conditions and in vivo cellular responses to external stimuli compared to the 2D monolayer. 77,78,80-82”
From Three-dimensional cell culture systems and their applications in drug discovery and cell-based biosensors (2014) · cites this paper as [78]
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- Authors
- Jeong, S. H., Lee, D. W., Kim, S., Kim, J., & Ku, B.
- Affiliations
- Samsung (South Korea) (KR)
- Gachon University (KR)
- Samsung Medical Center (KR)
- Corresponding author
- Jeong, Se Hoon
- Ku, Bosung
- Venue
- Biosensors and Bioelectronics
- Publisher
- Elsevier BV
- Published
- 2012-05
- Volume
- 35
- Issue
- 1
- Pages
- 128-133
- ISSN
- 0956-5663 (ISSN-L)
- OpenAlex
- W1987381349
- Cited by
- 33
- References
- 38
- Citations by year
- 2025: 2 · 2023: 4 · 2022: 2 · 2021: 3 · 2020: 2 · 2019: 1 · 2018: 6 · 2017: 3 · 2016: 1 · 2015: 3 · 2014: 4 · 2013: 2
- FWCI
- 1.72
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biosensors and Bioelectronics (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Chemistry › Electrochemistry › Electrochemical Analysis and Applications
- Physical Sciences › Engineering › Electrical and Electronic Engineering › Electrochemical sensors and biosensors
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced biosensing and bioanalysis techniques
- Keywords
- electrochemical biosensor, 3D cell culture, cytotoxicity assay, alginate, collagen, Matrigel, A549 cells, erlotinib, drug response monitoring, lung cancer
- MeSH terms
- Erlotinib Hydrochloride, Antineoplastic Agents, Cell Count, Cell Survival, Collagen, Drug Combinations, Gels, Humans, Laminin, Lung Neoplasms, Proteoglycans, Quinazolines, Biosensing Techniques, Spheroids, Cellular, Cell Culture Techniques, Cell Line, Tumor, Electrochemical Techniques
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