The DOI matches its Crossref record; 67 of the 67 references it cites were found in a registry or an index.
Cell-free protein synthesis: Applications come of age
Open accessAbstract
Cell-free protein synthesis has emerged as a powerful technology platform to help satisfy the growing demand for simple and efficient protein production. While used for decades as a foundational research tool for understanding transcription and translation, recent advances have made possible cost-effective microscale to manufacturing scale synthesis of complex proteins. Protein yields exceed grams protein produced per liter reaction volume, batch reactions last for multiple hours, costs have been reduced orders of magnitude, and reaction scale has reached the 100-liter milestone. These advances have inspired new applications in the synthesis of protein libraries for functional genomics and structural biology, the production of personalized medicines, and the expression of virus-like particles, among others. In the coming years, cell-free protein synthesis promises new industrial processes where short protein production timelines are crucial as well as innovative approaches to a wide range of applications.
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Cites
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Artificial Cells: Synthetic Compartments with Life-like Functionality and Adaptivity
Buddingh’, B. C., & van Hest, J. C. M. (2017) · Accounts of Chemical Research · doi:10.1021/acs.accounts.6b00512 · Cited by 666
Cell-free gene expression: an expanded repertoire of applications
Silverman, A. D., Karim, A. S., & Jewett, M. C. (2020) · Nature Reviews Genetics · doi:10.1038/s41576-019-0186-3 · Cited by 593
Self-citationModulation of Intrinsically Disordered Protein Function by Post-translational Modifications
Bah, A., & Forman-Kay, J. D. (2016) · Journal of Biological Chemistry · doi:10.1074/jbc.r115.695056 · Cited by 566
Cell-free synthetic biology: Thinking outside the cell
Hodgman, C. E., & Jewett, M. C. (2012) · Metabolic Engineering · doi:10.1016/j.ymben.2011.09.002 · Cited by 425
Self-citationAutonomous dynamic control of DNA nanostructure self-assembly
Green, L. N., Subramanian, H. K. K., Mardanlou, V., Kim, J., Hariadi, R. F., & Franco, E. (2019) · Nature Chemistry · doi:10.1038/s41557-019-0251-8 · Cited by 264
Metadata
- Authors
- Carlson, E. D., Gan, R., Hodgman, C. E., & Jewett, M. C.
- Venue
- Biotechnology Advances
- Publisher
- Elsevier BV
- Published
- 2012-09
- Volume
- 30
- Issue
- 5
- Pages
- 1185-1194
- ISSN
- 0734-9750 (ISSN-L)
- OpenAlex
- W2052060414
- Cited by
- 706
- References
- 67
- FWCI
- 11.29
- Type
- journal article
- Language
- en
- Open access
- green
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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