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Cited by 88Bone Metabolism and DiseasesCellular Mechanics and InteractionsBone health and osteoporosis research

Effects of cyclic hydraulic pressure on osteocytes

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Liu, Chao, Zhao, Yan, Cheung, Wing-Yee, Gandhi, Ronak, Wang, Liyun, You, Lidan·2010·Bone
DOI 10.1016/j.bone.2010.02.006PMC PMC3417308
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Abstract

Bone is able to adapt its composition and structure in order to suit its mechanical environment. Osteocytes, bone cells embedded in the calcified matrix, are believed to be the mechanosensors and responsible for orchestrating the bone remodeling process. Recent in vitro studies have shown that osteocytes are able to sense and respond to substrate strain and fluid shear. However the capacity of osteocytes to sense cyclic hydraulic pressure (CHP) associated with physiological mechanical loading is not well understood. In this study, we subjected osteocyte-like MLO-Y4 cells to controlled CHP of 68 kPa at 0.5 Hz, and investigated the effects of CHP on intracellular calcium concentration, cytoskeleton organization, mRNA expression of genes related to bone remodeling, and osteocyte apoptosis. We found that osteocytes were able to sense CHP and respond by increased intracellular calcium concentration, altered microtubule organization, a time-dependent increase in COX-2 mRNA level and RANKL/OPG mRNA ratio, and decreased apoptosis. These findings support the hypothesis that loading induced cyclic hydraulic pressure in bone serves as a mechanical stimulus to osteocytes and may play a role in regulating bone remodeling in vivo.

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  • In addition to gravity, mechanotransduction in bone is mediated by fluid shear stresses, hydrostatic pressures, and the force of muscular contraction.

    “In addition to the force of gravity, mechanotransduction in bone is mediated by fluid shear stresses [50], hydrostatic pressures [51], and the force of muscular contraction [52].”

    From Osteoclasts and Microgravity (2020) · cites this paper as [51]

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Cited by

Sorted by citations

Authors
Liu, C., Zhao, Y., Cheung, W.-Y., Gandhi, R., Wang, L., & You, L.
Affiliations
  • University of Toronto (CA)
  • University of Delaware (US)
Corresponding author
You, Lidan
ORCID
Venue
Bone
Publisher
Elsevier BV
Published
2010-05
Volume
46
Issue
5
Pages
1449-1456
ISSN
  • 1873-2763 (ISSN-L)
  • 8756-3282
DOI
10.1016/j.bone.2010.02.006
PMC
PMC3417308
OpenAlex
W2046832422
Cited by
88
References
9
Citations by year
2026: 7 · 2025: 3 · 2024: 2 · 2023: 2 · 2022: 10 · 2021: 5 · 2020: 6 · 2019: 5 · 2018: 7 · 2017: 5 · 2016: 4 · 2015: 6 · 2014: 3 · 2013: 9 · 2012: 6
FWCI
2.79
Type
journal article
Language
en
Open access
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Bone (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
  • TSpace (University of Toronto) (submittedVersion, cc-by-nc-nd)
  • Library and Archives Canada (Government of Canada) (submittedVersion)
Funders
  • NSERC
  • CFI
  • National Institute of Health (AR054385)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
  • Health Sciences › Medicine › Orthopedics and Sports Medicine › Bone health and osteoporosis research
Keywords
osteocytes, mechanotransduction, bone remodeling, intracellular calcium, microtubule organization, cytoskeleton, cyclooxygenase-2, RANKL/OPG ratio, osteocyte apoptosis, MLO-Y4 cells, mechanical loading, fluid shear
MeSH terms
Animals, Calcium, Cell Line, Cytoskeleton, Microtubules, Models, Theoretical, Osteocytes, Pressure, Bone Remodeling, Apoptosis, Shear Strength, Mice, Cyclooxygenase 2, RANK Ligand

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