The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Characterization of Engineered Channelrhodopsin Variants with Improved Properties and Kinetics
Open accessAbstract
Channelrhodopsin 2 (ChR2), a light-activated nonselective cationic channel from Chlamydomonas reinhardtii, has become a useful tool to excite neurons into which it is transfected. The other ChR from Chlamydomonas, ChR1, has attracted less attention because of its proton-selective permeability. By making chimeras of the transmembrane domains of ChR1 and ChR2, combined with site-directed mutagenesis, we developed a ChR variant, named ChEF, that exhibits significantly less inactivation during persistent light stimulation. ChEF undergoes only 33% inactivation, compared with 77% for ChR2. Point mutation of Ile(170) of ChEF to Val (yielding "ChIEF") accelerates the rate of channel closure while retaining reduced inactivation, leading to more consistent responses when stimulated above 25 Hz in both HEK293 cells and cultured hippocampal neurons. In addition, these variants have altered spectral responses, light sensitivity, and channel selectivity. ChEF and ChIEF allow more precise temporal control of depolarization, and can induce action potential trains that more closely resemble natural spiking patterns.
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Shaner, N. C., Campbell, R. E., Steinbach, P. A., Giepmans, B. N. G., Palmer, A. E., & Tsien, R. Y. (2004) · Nature Biotechnology · doi:10.1038/nbt1037 · Cited by 4,619
Self-citationChannelrhodopsin-2-assisted circuit mapping of long-range callosal projections
Petreanu, L., Huber, D., Sobczyk, A., & Svoboda, K. (2007) · Nature Neuroscience · doi:10.1038/nn1891 · Cited by 930
Sparse optical microstimulation in barrel cortex drives learned behaviour in freely moving mice
Huber, D., Petreanu, L., Ghitani, N., Ranade, S., Hromádka, T., Mainen, Z., & Svoboda, K. (2008) · Nature · doi:10.1038/nature06445 · Cited by 539
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Nabavi, S., Fox, R., Proulx, C. D., Lin, J. Y., Tsien, R. Y., & Malinow, R. (2014) · Nature · doi:10.1038/nature13294 · Cited by 980
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Tee, B. C.-K., Chortos, A., Berndt, A., Nguyen, A. K., Tom, A., McGuire, A., Lin, Z. C., Tien, K., Bae, W.-G., Wang, H., et al. (2015) · Science · doi:10.1126/science.aaa9306 · Cited by 882
Metadata
- Authors
- Lin, J. Y., Lin, M. Z., Steinbach, P., & Tsien, R. Y.
- Venue
- Biophysical Journal
- Publisher
- Elsevier BV
- Published
- 2009-03
- Volume
- 96
- Issue
- 5
- Pages
- 1803-1814
- ISSN
- 0006-3495 (ISSN-L)
- OpenAlex
- W2088944569
- Cited by
- 697
- References
- 3
- FWCI
- 13.93
- Type
- journal article
- Language
- en
- Open access
- hybrid
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