Cited by 930Neuroscience and Neuropharmacology ResearchPhotoreceptor and optogenetics researchNeuroscience and Neural Engineering

Channelrhodopsin-2-assisted circuit mapping of long-range callosal projections

Petreanu, Leopoldo, Huber, Daniel, Sobczyk, Aleksander, Svoboda, Karel·2007·Nature Neuroscience

Abstract

The functions of cortical areas depend on their inputs and outputs, but the detailed circuits made by long-range projections are unknown. We show that the light-gated channel channelrhodopsin-2 (ChR2) is delivered to axons in pyramidal neurons in vivo. In brain slices from ChR2-expressing mice, photostimulation of ChR2-positive axons can be transduced reliably into single action potentials. Combining photostimulation with whole-cell recordings of synaptic currents makes it possible to map circuits between presynaptic neurons, defined by ChR2 expression, and postsynaptic neurons, defined by targeted patching. We applied this technique, ChR2-assisted circuit mapping (CRACM), to map long-range callosal projections from layer (L) 2/3 of the somatosensory cortex. L2/3 axons connect with neurons in L5, L2/3 and L6, but not L4, in both ipsilateral and contralateral cortex. In both hemispheres the L2/3-to-L5 projection is stronger than the L2/3-to-L2/3 projection. Our results suggest that laminar specificity may be identical for local and long-range cortical projections.

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Crossref · 10.1038/nn1891

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  • The EPSCs represent monosynaptic glutamate release from DRN DA terminals.

    “Furthermore, the EPSCs persisted in the presence of tetradotoxin (TTX) and 4-aminopyridine (4AP), suggesting that they represent monosynaptic glutamate release from DRN DA terminals (Petreanu et al., 2007).”

    From Dorsal raphe dopamine neurons represent the experience of social isolation (2016) · cites this paper as Petreanu et al., 2007

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Authors
Petreanu, L., Huber, D., Sobczyk, A., & Svoboda, K.
Affiliations
Howard Hughes Medical Institute (US)
Corresponding author
Svoboda, Karel
Venue
Nature Neuroscience
Published
2007-04-15
Volume
10
Issue
5
Pages
663-668
ISSN
  • 1097-6256 (ISSN-L)
  • 1546-1726
OpenAlex
W2163438163
Cited by
930
References
46
Citations by year
2026: 38 · 2025: 38 · 2024: 56 · 2023: 44 · 2022: 56 · 2021: 49 · 2020: 44 · 2019: 40 · 2018: 50 · 2017: 52 · 2016: 54 · 2015: 55 · 2014: 58 · 2013: 57 · 2012: 56
FWCI
15.33
Type
journal article
Language
en
Open access
closed
Locations
  • Nature Neuroscience (publishedVersion)
  • PubMed (publishedVersion)
  • Cold Spring Harbor Laboratory Institutional Repository (Cold Spring Harbor Laboratory) (acceptedVersion)
Funders
National Institutes of Health
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Photoreceptor and optogenetics research
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neural Engineering
Keywords
channelrhodopsin-2, circuit mapping, somatosensory cortex, layer 2/3 pyramidal neurons, laminar specificity, cortical connectivity
MeSH terms
Animals, Animals, Newborn, Axons, Brain Mapping, Corpus Callosum, Embryo, Mammalian, Female, Functional Laterality, Membrane Potentials, Mice, Inbred C57BL, Mice, Transgenic, Nerve Net, Neural Pathways, Photic Stimulation, Pregnancy, Retinaldehyde, Rhodopsin, Somatosensory Cortex, Patch-Clamp Techniques, Mice, In Vitro Techniques

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