Neuroanatomical distribution and colocalisation of nuclear receptor corepressor (N-CoR) and silencing mediator of retinoid and thyroid receptors (SMRT) in rat brain.
Abstract
The two structurally related nuclear receptor corepressor (N-CoR) and silencing mediator of retinoid and thyroid receptors (SMRT) proteins have been found to differentially affect the transcriptional activity of numerous nuclear receptors, such as thyroid hormone, retinoic acid and steroid receptors. Because of the numerous effects mediated by nuclear receptors in brain, it is of interest to extend these in vitro data and to explore the cellular distribution of both corepressors in brain tissue. We therefore examined, using in situ hybridisation, whether the relative abundance of these two functionally distinct corepressors differed in rat brain and pituitary. We find that although both N-CoR and SMRT transcripts are ubiquitously expressed in brain, striking differences in their respective levels of expression could be observed in discrete areas of brain stem, thalamus, hypothalamus and hippocampus. Using dual-label immunofluorescence, we examined in selected glucocorticoid sensitive areas involved in the regulation of the hypothalamus-pituitary-adrenal axis activity, the respective protein abundance of N-CoR and SMRT. Protein abundance was largely concurrent with the mRNA expression levels, with SMRT relatively more abundant in hypothalamus and brain stem areas. Colocalisation of N-CoR and SMRT was demonstrated by confocal microscopy in most areas studied. Taken together, these findings are consistent with the idea that the uneven neuroanatomical distribution of N-CoR and SM…
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NCoR mRNA is ubiquitously distributed in the adult rat brain.
“Although NCoR mRNA has been found to be ubiquitously distributed (27) in the adult rat brain, we now show that females express higher levels of the corepressor, NCoR, within the MBH and amygdala during development.”
From The Nuclear Receptor Corepressor Has Organizational Effects within the Developing Amygdala on Juvenile Social Play and Anxiety-Like Behavior (2010) · cites this paper as [27]
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- Authors
- van der Laan, S., Lachize, S. B., Schouten, T. G., Vreugdenhil, E., de Kloet, E. R., & Meijer, O. C.
- Affiliations
- Leiden University Medical Center (NL)
- Venue
- Brain Research
- Publisher
- Elsevier BV
- Published
- 2005-10
- Volume
- 1059
- Issue
- 2
- Pages
- 113-121
- ISSN
- 0006-8993 (ISSN-L)
- OpenAlex
- W1994398354
- Cited by
- 27
- References
- 0
- Citations by year
- 2025: 1 · 2024: 1 · 2021: 1 · 2020: 3 · 2017: 2 · 2015: 1 · 2013: 1 · 2012: 1
- FWCI
- 1.25
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Brain Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Koninklijke Nederlandse Akademie van Wetenschappen
- Nederlandse Organisatie voor Wetenschappelijk Onderzoek
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
- Life Sciences › Neuroscience › Behavioral Neuroscience › Stress Responses and Cortisol
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Nuclear Receptors and Signaling
- Keywords
- nuclear receptor corepressor, SMRT, neuroanatomical distribution, colocalization, hypothalamus, brainstem, hippocampus, thalamus, pituitary gland, hypothalamic-pituitary-adrenal axis, glucocorticoids, gene expression, rat brain
- MeSH terms
- Animals, Brain, Brain Mapping, DNA-Binding Proteins, Hypothalamo-Hypophyseal System, Male, Nuclear Proteins, Pituitary Gland, Pituitary-Adrenal System, Repressor Proteins, RNA, Messenger, Tissue Distribution, Transcription, Genetic, Signal Transduction, Rats, Wistar, In Situ Hybridization, Rats, Nuclear Receptor Co-Repressor 1, Nuclear Receptor Co-Repressor 2
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